Patent US4600302A successfully refreshed from Google Patents.
US4600302A
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Flow cytometry apparatus with uniform incoherent light excitation
Assignee: Becton Dickinson and Co
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Filing: 1984-03-26
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Granted: 1986-07-15
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Expires: 2004-03-26
Abstract
Abstract A flow cytometry apparatus for determining one or more characteristics of particles or the like flowing in a liquid stream includes a nozzle for generating a liquid flow stream for moving particles therethrough substantially one at a time. An arc lamp provides a beam of incoherent light to illuminate the particles moving in the flow stream. A prism in the light beam modifies non-uniform incoherent light, as provided by said arc lamp, to a more uniform beam of illumination which is directed to the flowing particles. A detector is included for detecting light with respect to each moving particle and for associating the detected light with one or more characteristics of the particles.
Inventors
- 👤 Burton H. Sage, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 14:39
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1434; G01N15/1468; G01N15/147; G01N2015/1497; G01N2201/00; G01N2201/06; G01N2201/063; G01N2201/0631
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
157 words
A flow cytometry apparatus for determining characteristics of cells or the like flowing in a liquid stream comprising: means for moving cells, substantially one at a time, in a liquid flow stream; an excitation light source for providing a beam of incoherent light to illuminate said cells moving in said flow stream; an elongate, solid, light transmissive prism having its longitudinal axis aligned substantially along said light beam between said source and said flow stream, said prism having internally reflective side surfaces and light transmissive end surfaces so that non-uniform incoherent light from said source directed into said prism through one of its end surfaces exits said prism through the opposite end surface in uniform fashion as the light is directed toward said cells; means for detecting light associated with each moving cell as it passes through said area of illumination; and means for using said detected light to determine one or more characteristics of said cells.
Claim 2
Dependent Claim
157 words
A flow cytometry apparatus for determining characteristics of cells or the like flowing in a liquid stream comprising: means for moving cells, substantially one at a time, in a liquid flow stream; an excitation light source for providing a beam of incoherent light to illuminate said cells moving in said flow stream; an elongate, solid, light transmissive prism having its longitudinal axis aligned substantially along said light beam between said source and said flow stream, said prism having internally reflective side surfaces and light transmissive end surfaces so that non-uniform incoherent light from said source directed into said prism through one of its end surfaces exits said prism through the opposite end surface in uniform fashion as the light is directed toward said cells; means for detecting light associated with each moving cell as it passes through said area of illumination; and means for using said detected light to determine one or more characteristics of said cells.
Claim 3
Dependent Claim
12 words
The apparatus of claim 1 wherein said prism is rectangular in cross-section.
Claim 4
Dependent Claim
19 words
The apparatus of claim 1 wherein said prism is positioned in said beam of light adjacent said light source.
Claim 5
Dependent Claim
18 words
The apparatus of claim 1 wherein the cross-section of said prism is substantially constant along its longitudinal axis.
Claim 6
Dependent Claim
34 words
The apparatus of claim 4 wherein the maximum cross-sectional dimension of said prism is greater than the width of the incident beam of incoherent light adapted to enter one end surface of the prism.
Claim 7
Dependent Claim
26 words
The apparatus of claim 1 wherein the end surfaces of said prism are treated with an anti-reflectance agent to improve the efficiency of light transmission therethrough.
Claim 8
Dependent Claim
30 words
The apparatus of claim 1 which further includes a lens positioned between said light source and said prism for focusing light into the prism through said one end surface thereof.
Claim 9
Dependent Claim
19 words
The apparatus of claim 7 wherein said lens is in contact with said one end surface of said prism.
Claim 10
Dependent Claim
18 words
The apparatus of claim 8 wherein said prism and said lens are integrally formed from the same material.
Claim 11
Dependent Claim
12 words
The apparatus of claim 1 wherein said prism is made of quartz.
Claim 12
Dependent Claim
16 words
The apparatus of claim 9 wherein the integrally formed prism and lens is made of quartz.
Claim 13
Dependent Claim
27 words
The apparatus of claim 1 wherein said means for detecting light includes a device for detecting fluorescence emitted by the cells passing through said area of illumination.
Claim 14
Dependent Claim
27 words
The apparatus of claim 1 wherein said means for detecting light includes a device for detecting light scattered by the cells passing through said area of illumination.
Claim 15
Dependent Claim
186 words
A flow cytometry apparatus for determining characteristics of cells or the like flowing in a liquid stream comprising: means for moving cells, substantially one at a time, in a liquid flow stream; an arc lamp for providing a beam of incoherent light to illuminate said cells moving in said flow stream; an elongate, rectangular cross-section, solid, light transmissive prism having its longitudinal axis aligned substantially along said light beam and positioned between said lamp and said flow stream, said prism having internally reflective side surfaces and light transmissive end surfaces so that non-uniform light from said lamp directed to said prism through a first end surface exists said prism through the opposite, second end surface in uniform fashion toward said cells; a lens in contact with said first end surface of said prism and positioned adjacent to and facing said lamp for focusing light into the prism through said first end surface thereof; means for detecting light associated with each moving cell as it passes through said area of illumination; and means for using said detected light to determine one or more characteristics of said cells.
Claim 16
Dependent Claim
186 words
A flow cytometry apparatus for determining characteristics of cells or the like flowing in a liquid stream comprising: means for moving cells, substantially one at a time, in a liquid flow stream; an arc lamp for providing a beam of incoherent light to illuminate said cells moving in said flow stream; an elongate, rectangular cross-section, solid, light transmissive prism having its longitudinal axis aligned substantially along said light beam and positioned between said lamp and said flow stream, said prism having internally reflective side surfaces and light transmissive end surfaces so that non-uniform light from said lamp directed to said prism through a first end surface exists said prism through the opposite, second end surface in uniform fashion toward said cells; a lens in contact with said first end surface of said prism and positioned adjacent to and facing said lamp for focusing light into the prism through said first end surface thereof; means for detecting light associated with each moving cell as it passes through said area of illumination; and means for using said detected light to determine one or more characteristics of said cells.
Claim 17
Dependent Claim
14 words
The apparatus of claim 14 wherein said arc lamp is a mercury arc lamp.
Claim 18
Dependent Claim
17 words
The apparatus of claim 14 wherein the outside of said side surfaces has a protective coating thereon.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-07-14 | 1990-01-13 | 1990-07-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-07-14 | 1994-01-13 | 1994-07-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-07-14 | 1998-01-13 | 1998-07-15 | $7700.00 | Window Closed / Lapsed |