Patent US4597944A successfully refreshed from Google Patents.
US4597944A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Agglutination reagent detection system
Assignee: Individual
•
Filing: 1983-10-18
•
Granted: 1986-07-01
•
Expires: 2003-10-18
Abstract
Abstract A reagent detection system for detecting an agglutination reaction in a reagent is provided. A light source directs monochromatic light toward an isoplanar sample field. A detector is provided for detecting a change in the amount of light scattered from the isoplanar sample field caused by the agglutination reaction of the reagents. The agglutination reaction is controlled by the intervention of a kinetic activator and results in consistently reproducible sensitive detection of the agglutination reaction.
Inventors
- 👤 Hugh V. Cottingham
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 17:46
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/75; G01N21/77; G01N21/82
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
111 words
A reagent detection system for detecting a particulate agglutination reaction in an reagent comprising, in combination, sample field means for receiving a particulate liquid agglutination reagent and defining a liquid isoplanar sample field, light means for directing light towards said liquid isoplanar sample field, and detection means cooperating with said light means for instantaneously detecting a change in the amount of light scattered from said liquid isoplanar sample field caused by the agglutination reaction by summing light scattered from substantially all particles in the sample field and in response thereto for producing a display signal that is substantially linearly related to the surface area of the particles as modified by agglutination.
Claim 2
Dependent Claim
111 words
A reagent detection system for detecting a particulate agglutination reaction in an reagent comprising, in combination, sample field means for receiving a particulate liquid agglutination reagent and defining a liquid isoplanar sample field, light means for directing light towards said liquid isoplanar sample field, and detection means cooperating with said light means for instantaneously detecting a change in the amount of light scattered from said liquid isoplanar sample field caused by the agglutination reaction by summing light scattered from substantially all particles in the sample field and in response thereto for producing a display signal that is substantially linearly related to the surface area of the particles as modified by agglutination.
Claim 3
Dependent Claim
34 words
A reagent detection system, as claimed in claim 1, and including kinetic energy activation means for kinetically activating said liquid sample reagent in said sample field to thereby control the agglutination reaction caused thereby.
Claim 4
Dependent Claim
49 words
A reagent detection system, as claimed in claim 2, wherein said kinetic activation means kinetically activates said reagents for a predetermined interval of time to define a controlled reaction, said detection means being adapted to detect said reagent at the beginning and at the end of said predetermined interval.
Claim 5
Dependent Claim
51 words
A reagent detection system, as claimed in claim 1, 2 or 3, wherein said sample field means for receiving said reagents and defining said liquid isoplanar sample field includes a wettable surface means having a discrete area, said detection means being adapted to detect said light scattered from said discrete area.
Claim 6
Dependent Claim
35 words
A reagent detection system, as claimed in claim 4, wherein said light means is a monochromatic light source, said monochromatic light source directing light at said entire discrete area defining said liquid isoplanar sample field.
Claim 7
Dependent Claim
37 words
A reagent detection system, as claimed in claim 5, wherein said detection means is positioned normal with respect to said isoplanar sample field and is centered with respect to said discrete area defining said isoplanar sample field.
Claim 8
Dependent Claim
37 words
A reagent detection system as claimed in claim 6, wherein said monochromatic light source is disposed at an angle with respect to said detection means to effect detection of a single scattering effect by said detection means.
Claim 9
Dependent Claim
43 words
A reagent detection system, as claimed in claim 6, wherein said monochromatic light source is disposed to direct monochromatic light at an angle of 40° with respect to the position defined by positioning of said detection means with respect to said discrete area.
Claim 10
Dependent Claim
85 words
A reagent detection system, as claimed in claim 3, wherein said display signal produced by said detection means includes a first analog signal that is representative of the state of the agglutination reaction of the reagent in the liquid isoplanar sample field at the beginning of said predetermined interval and said display signal further includes a second analog signal representative of the state of the agglutination reaction of said reagent in the liquid isoplanar sample field at the end of said predetermined interval of time.
Claim 11
Dependent Claim
46 words
A reagent detection system, as claimed in claim 9, and including comparator means for comparing said first analog signal and said second analog signal and for producing a comparative signal representative of any change in level between said first analog signal and said second analog signal.
Claim 12
Dependent Claim
45 words
A reagent detection system, as claimed in claim 10, and including display means for receiving said comparative signals and providing a display representative of one of the difference and lack of difference between the level of said first analog signal and said second analog signal.
Claim 13
Dependent Claim
44 words
A reagent detection system, as claimed in claim 2 or 3, wherein said kinetic activation means is an air jet for directing air impulses at a predetermined position of said liquid isoplanar sample field to effect movement of said reagent in a predetermined manner.
Claim 14
Dependent Claim
89 words
A reagent detection system, as claimed in claim 12, wherein said detection means is adapted to produce a first analog signal that is representative of the state of the agglutination reaction of the reagent in the liquid isoplanar sample field at the beginning of said predetermined interval of time and said detection means is further adapted to produce a second analog signal representative of a second state of the agglutination reaction of said reagent in the liquid isoplanar sample field at the end of said predetermined interval of time.
Claim 15
Dependent Claim
90 words
A reagent detection system, as claimed in claim 13, and including comparator means for comparing said first analog signal and said second analog signal and for producing a comparative signal representative of the change in level between said first analog signal and said second analog signal, said signal being representative of the difference in the voltage level of said first analog signal and said second analog signal as compared to the amount of kinetic activation of said reagent in said liquid isoplanar sample field during said predetermined interval of time.
References Cited (Backward Citations)
US3065665A
Cited
US3398935A
Cited
US3656833A
Cited
US3736042A
Cited
US3819271A
Cited
US4059405A
Cited
US4059405B1
Cited
US4125828A
Cited
US3904781A
Cited
US3905767A
Cited
US4055768A
Cited
US4252536A
Cited
US4174952A
Cited
US4205954A
Cited
US4452902A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-06-30 | 1989-12-30 | 1990-07-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-06-30 | 1993-12-30 | 1994-07-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-06-30 | 1997-12-30 | 1998-07-01 | $7700.00 | Window Closed / Lapsed |