US4591572A
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US
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Pigmentation associated, differentiation antigen of human melanoma and autologous antibody
Assignee: Memorial Sloan Kettering Cancer Center
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Filing: 1983-04-01
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Granted: 1986-05-27
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Expires: 2003-04-01
Abstract
Abstract The present invention concerns an autologous precipitating antibody and the gp70 pigment-associated antigen on melanoma cells which it recognizes. The antibody is useful in detecting pigmented melanoma cells in excised specimen, serum or urine.
Inventors
- 👤 M. Jules Mattes
- 👤 Timothy M. Thomson
- 👤 Lloyd J. Old
- 👤 Kenneth O. Lloyd
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 00:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/575; G01N33/5751; A; A61; A61K; A61K39/00; G01N2474/00; G01N2474/20; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/813; Y10S436/815; Y10S436/823; Y10S436/828; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
24 words
Substantially pure antibody which specifically binds to the pigmentation-associated gp70 antigen specific to melanocytes and melanomas, said antigen having an isoelectric point of 5.2.
Claim 2
Dependent Claim
24 words
Substantially pure antibody which specifically binds to the pigmentation-associated gp70 antigen specific to melanocytes and melanomas, said antigen having an isoelectric point of 5.2.
Claim 3
Dependent Claim
11 words
Antibody of claim 1 labelled with a radioactive or chromophoric group.
Claim 4
Dependent Claim
10 words
Antibody of claim 1 immobilized on a solid inert support.
Claim 5
Dependent Claim
24 words
Substantially pure pigmentation associated gp 70 antigen specific to melanocytes and melanomas, said antigen having an isoelectric point of 5.2, isolated from melanoma cells.
Claim 6
Dependent Claim
24 words
Substantially pure pigmentation associated gp 70 antigen specific to melanocytes and melanomas, said antigen having an isoelectric point of 5.2, isolated from melanoma cells.
Claim 7
Dependent Claim
52 words
Method of isolating the pigment-associated gp 70 antigen specific to melanocytes and melanomas, said antigen having an isoelectric point of 5.2, comprising immunoprecipitation of a melanoma cell extract with antibodies which specifically bind to said pigment associated gp 70 antigen specific to melanocytes and melanomas and separating said immunoprecipitate from said extract.
Claim 8
Dependent Claim
52 words
Method of isolating the pigment-associated gp 70 antigen specific to melanocytes and melanomas, said antigen having an isoelectric point of 5.2, comprising immunoprecipitation of a melanoma cell extract with antibodies which specifically bind to said pigment associated gp 70 antigen specific to melanocytes and melanomas and separating said immunoprecipitate from said extract.
Claim 9
Dependent Claim
16 words
Method of claim 5 wherein said melanoma cell extract is radiolabelled and said immunoprecipitation is radioimmunoprecipitation.
Claim 10
Dependent Claim
25 words
Pigmentation associated gp 70 antigen specific to melanocytes and melanomas, said antigen having an isoelectric point of 5.2, isolated by the method of claim 6.
Claim 11
Dependent Claim
17 words
Method of claim 6 wherein said melanoma cell extract is extracted from cultured melanoma cell line SK-MEL-28.
Claim 12
Dependent Claim
31 words
Method for detecting pigmented melanoma cells comprising: (a) contacting a biological sample containing said cells with antibody of claim 1; and (b) detecting the reaction between the cells and the antibody.
Claim 13
Dependent Claim
11 words
Method of claim 9 wherein said biological sample is excised tissue.
Claim 14
Dependent Claim
11 words
Method of claim 9 wherein said biological sample is excised tumor.
Claim 15
Dependent Claim
19 words
Method of claim 9 wherein said biological sample is blood or urine from an individual suspected of having melanoma.
Claim 16
Dependent Claim
53 words
Method for detecting pigmented melanoma cells in the serum of an individual comprising: (a) contacting said serum with antibody which specifically binds to the pigmentation associated gp 70 antigen specific to melanocytes and melanomas, said antigen having an isoelectric point of 5.2; and (b) detecting the reaction between the cells and the antibody.
Claim 17
Dependent Claim
53 words
Method for detecting pigmented melanoma cells in the serum of an individual comprising: (a) contacting said serum with antibody which specifically binds to the pigmentation associated gp 70 antigen specific to melanocytes and melanomas, said antigen having an isoelectric point of 5.2; and (b) detecting the reaction between the cells and the antibody.
Claim 18
Dependent Claim
48 words
Method for detecting cells in an early stage of differentiation comprising: (a) contacting said cells with antibody which specifically binds to the pigmentation associated gp 70 antigen specific to melanocytes and melanomas having an isoelectric point of 5.2; and (b) detecting binding between the cells and the antibody.
Claim 19
Dependent Claim
48 words
Method for detecting cells in an early stage of differentiation comprising: (a) contacting said cells with antibody which specifically binds to the pigmentation associated gp 70 antigen specific to melanocytes and melanomas having an isoelectric point of 5.2; and (b) detecting binding between the cells and the antibody.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-26 | 1989-11-25 | 1990-05-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-26 | 1993-11-25 | 1994-05-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-26 | 1997-11-25 | 1998-05-27 | $7700.00 | Window Closed / Lapsed |