Patent US4589965A successfully refreshed from Google Patents.
US4589965A
Expired / Lapsed
US
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Method for electroblotting
Assignee: International Biotechnologies Inc
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Filing: 1984-11-14
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Granted: 1986-05-20
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Expires: 2004-11-14
Abstract
Abstract A rapid and effective method for electroblotting is provided whereby an electrophoretically resolved material in a gelatin sheet is quickly and efficiently transferred to a membrane with high pattern definition and resolution.
Inventors
- 👤 John H. Kreisher
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 09:53
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/4473
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
86 words
A method for rapid molecular transfer without immersion in a buffer solution which comprises: providing an electrophoretically resolved material in a gelatin sheet; placing said gelatin sheet in contacting relationship with an immobilizing material to form a sheet-material combination; sandwiching said sheet-material combination between two plate electrodes in direct face-to-face relationship therewith to form an assembly; and applying electric current to said electrodes to provide transfer of electrophoretically resolved material to the immobilizing material without immersion in a buffer solution and in short periods of time.
Claim 2
Independent Claim
86 words
A method for rapid molecular transfer without immersion in a buffer solution which comprises: providing an electrophoretically resolved material in a gelatin sheet; placing said gelatin sheet in contacting relationship with an immobilizing material to form a sheet-material combination; sandwiching said sheet-material combination between two plate electrodes in direct face-to-face relationship therewith to form an assembly; and applying electric current to said electrodes to provide transfer of electrophoretically resolved material to the immobilizing material without immersion in a buffer solution and in short periods of time.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein said immobilizing material is paper.
Claim 4
Dependent Claim
11 words
The method of claim 1 wherein said immobilizing material is nylon.
Claim 5
Dependent Claim
17 words
The method of claim 1 wherein said sheet-material combination is in contacting relationship with said plate electrodes.
Claim 6
Dependent Claim
24 words
The method of claim 1 wherein a small amount of a non-reactive, conductive buffer solution is placed between the plate electrodes and sheet-material combination.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein said assembly is horizontally disposed.
Claim 8
Dependent Claim
12 words
The method of claim 1 wherein said plate electrodes are flat plates.
Claim 9
Dependent Claim
13 words
The method of claim 7 wherein said plate electrodes are flat carbon plates.
Claim 10
Dependent Claim
16 words
The method of claim 1 wherein said plate electrodes include holes therein for release of gases.
Claim 11
Dependent Claim
16 words
The method of claim 1 wherein said plate electrodes include grooves therein for release of gases.
Claim 12
Dependent Claim
21 words
The method of claim 1 wherein said plate electrodes are able to conduct amperages as high as 20 amps without heat-up.
Claim 13
Dependent Claim
12 words
The method of claim 1 wherein said assembly is placed under compression.
References Cited (Backward Citations)
WO1981002790A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-19 | 1989-11-18 | 1990-05-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-19 | 1993-11-18 | 1994-05-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-19 | 1997-11-18 | 1998-05-20 | $7700.00 | Window Closed / Lapsed |