US4582860A
Expired / Lapsed
US
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Oxirane resins for enzyme immobilization
Assignee: Rohm and Haas Co
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Filing: 1983-12-15
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Granted: 1986-04-15
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Expires: 2003-12-15
Abstract
Abstract Carriers for immobilizing enzymes may be prepared by suspension copolymerizing an oxirane-group-bearing, monovinyl monomer and a major amount of a trivinyl crosslinking monomer having a hydrophilic character, in the presence of a phase separator which does not react with the oxirane group. The resulting carriers, in bead form, have high porosity, high surface area, and pores of diameter sufficient for ready penetration by enzymes and substrates.
Inventors
- 👤 Michael P. Bigwood
- 👤 John O. Naples
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 06:07
CPC Classifications
C; C08; C08F; C08F216/00; C08F216/12; C08F216/14; C08F220/00; C08F220/02; C08F220/10; C08F220/26; C08F220/32; C08F220/325; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
99 words
A process for preparing an improved porous carrier bead for immobilized enzymes which process consists essentially of free-radical, suspension polymerizing an oxirane-group-bearing monovinyl monomer selected from the group consisting of glycidyl acrylate, glycidyl methacrylate, allyl glycidyl ether, and mixtures thereof, and a trivinyl crosslinking monomer having hydrophilic character, the ratio of monovinyl monomer to trivinyl monomer being from about 5:95 to about 50:50 by weight, in the presence of about 20% to about 90%, based on the weight of the organic phase, of a phase separator which does not react with the oxirane group, in an aqueous suspension medium.
Claim 2
Dependent Claim
99 words
A process for preparing an improved porous carrier bead for immobilized enzymes which process consists essentially of free-radical, suspension polymerizing an oxirane-group-bearing monovinyl monomer selected from the group consisting of glycidyl acrylate, glycidyl methacrylate, allyl glycidyl ether, and mixtures thereof, and a trivinyl crosslinking monomer having hydrophilic character, the ratio of monovinyl monomer to trivinyl monomer being from about 5:95 to about 50:50 by weight, in the presence of about 20% to about 90%, based on the weight of the organic phase, of a phase separator which does not react with the oxirane group, in an aqueous suspension medium.
Claim 3
Dependent Claim
21 words
The process of claim 1 wherein the ratio of monovinyl monomer to trivinyl monomer is from about 10:90 to about 30:70.
Claim 4
Dependent Claim
25 words
The process of claim 1 wherein the crosslinking monomer is selected from the group consisting of trimethylolpropane trimethacrylate, trimethylolpropane triacrylate, triallyl isocyanurate, or mixtures thereof.
Claim 5
Dependent Claim
14 words
The process of claim 1 wherein the phase separator is a hydrocarbon phase separator.
Claim 6
Dependent Claim
12 words
The process of claim 4 wherein the hydrocarbon phase separator is toluene.
Claim 7
Dependent Claim
13 words
The oxirane-group-bearing carrier for immobilized enzymes prepared by the process of claim 1.
References Cited (Backward Citations)
US4070348A
Cited
US4246351A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-04-14 | 1989-10-14 | 1990-04-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-04-14 | 1993-10-14 | 1994-04-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-04-14 | 1997-10-14 | 1998-04-15 | $7700.00 | Window Closed / Lapsed |