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Patent US4577964A successfully refreshed from Google Patents.
US4577964A
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US
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Apparatus and method for detecting platelets in whole blood
Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1978-09-06
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Granted: 1986-03-25
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Expires: 1998-09-06
Abstract
Abstract A diluted whole blood sample is hydrodynamically focused to a given point. A light beam is transversely focused on the same point and the principles of dark field microscopy are utilized, detecting scattered light and thereby discriminating platelets from red blood cells based on cell volume and refractivity.
Inventors
- 👤 William P. Hansen, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 22:41
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/02; G01N15/0205
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
90 words
A method of analyzing blood cells of a sample comprising: (a) providing all cells to be analyzed through a predetermined area in a single sample stream analysis; (b) illuminating respective blood cells in said sample by providing to said area focused illumination zone which dimensionally accommodates both red cells and platelets; (c) detecting light scattered by cells in said zone to the exclusion of other light; and (d) discriminating platelets from red blood cells based on the parameters of cell volume and cell refractivity and duration of light so detected.
Claim 2
Independent Claim
90 words
A method of analyzing blood cells of a sample comprising: (a) providing all cells to be analyzed through a predetermined area in a single sample stream analysis; (b) illuminating respective blood cells in said sample by providing to said area focused illumination zone which dimensionally accommodates both red cells and platelets; (c) detecting light scattered by cells in said zone to the exclusion of other light; and (d) discriminating platelets from red blood cells based on the parameters of cell volume and cell refractivity and duration of light so detected.
Claim 3
Dependent Claim
61 words
The method of claim 1 wherein said illuminating step comprises: (a) passing the cells, one at a time, past a predetermined focal point; (b) providing a regulated source of visible light; (c) focusing light from said source substantially normally to cell flow to illuminate substantially single cells at said point, said focused light beam haivng a predetermined cross-section at said point.
Claim 4
Dependent Claim
35 words
The method of claim 2 wherein said blocking step comprises placing a physical block across the light path emergent from said cells, said block being placed in the focal plane eventuating from said illuminating step.
Claim 5
Dependent Claim
24 words
The method of claim 3 wherein said detecting step comprises detecting light emergent from said focal point and not blocked in said placing step.
Claim 6
Dependent Claim
48 words
A method as described in claim 1 wherein said illuminating step comprises focusing light normally to a sensing zone through which cells of said sample pass, in sequence, generally normally to incident light, said zone being elongated generally normally both to incident light and to the sample stream.
Claim 7
Independent Claim
57 words
The method of claims 1 or 5 wherein said detecting step comprises: (a) detecting substantially low-angle, forward-scattered light from cells; (b) blocking substantially all other light from said cells; and wherein said discriminating step comprises discriminating whether a red cell or a platelet scattered the detected light, based on the amplitude and duration of light so detected.
Claim 8
Independent Claim
84 words
A method of analyzing blood cells of a sample comprising: (a) providing all cells to be analyzed through a predetermined area in a single sample stream analysis; (b) illuminating respective blood cells in said sample by providing to said area a focused illumination zone which dimensionally accommodates both cells and platelets; (c) detecting light scattered by cells in said zone to the exclusion of other light; and (d) discriminating platelets from red blood cells based on the parameters of cell volume and cell refactivity.
Claim 9
Independent Claim
84 words
A method of analyzing blood cells of a sample comprising: (a) providing all cells to be analyzed through a predetermined area in a single sample stream analysis; (b) illuminating respective blood cells in said sample by providing to said area a focused illumination zone which dimensionally accommodates both cells and platelets; (c) detecting light scattered by cells in said zone to the exclusion of other light; and (d) discriminating platelets from red blood cells based on the parameters of cell volume and cell refactivity.
Claim 10
Dependent Claim
34 words
The method as provided in claim 7 wherein the detecting step comprises: (a) detecting substantially low angle, forward scattered light by said cells; and (b) blocking substantially all other light from said illumination zone.
Claim 11
Dependent Claim
25 words
The method as provided in claim 8 wherein the blocking step comprises placing a physical block across the light path emergent from said illumination zone.
Claim 12
Dependent Claim
24 words
The method as provided in claim 9 wherein the detecting step comprises detecting light emergent from said zone not blocked in said placing step.
Claim 13
Independent Claim
100 words
A method of analyzing blood cells of a sample comprising: (a) providing all cells to be analyzed through a predetermined area in a single sample stream analysis; (b) illuminating respective blood cells in said sample by providing to said area a focused illumination zone which dimensionally accommodates both cells and platelets; (c) detecting light scattered by cells in said zone to the exclusion of other light by blocking substantially nonscattered light and applying said scattered light to a dark field optics system; and (d) discriminating platelets from red blood cells based on the parameters of cell volume and cell refractivity.
Claim 14
Independent Claim
100 words
A method of analyzing blood cells of a sample comprising: (a) providing all cells to be analyzed through a predetermined area in a single sample stream analysis; (b) illuminating respective blood cells in said sample by providing to said area a focused illumination zone which dimensionally accommodates both cells and platelets; (c) detecting light scattered by cells in said zone to the exclusion of other light by blocking substantially nonscattered light and applying said scattered light to a dark field optics system; and (d) discriminating platelets from red blood cells based on the parameters of cell volume and cell refractivity.
References Cited (Backward Citations)
US4110604A
Cited
US4202625A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-03-24 | 1989-09-23 | 1990-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-03-24 | 1993-09-23 | 1994-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-03-24 | 1997-09-23 | 1998-03-25 | $7700.00 | Window Closed / Lapsed |