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US4575808A
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Method of recording densitogram representing densities of fractionated substances
Assignee: Olympus Corp
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Filing: 1983-07-14
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Granted: 1986-03-11
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Expires: 2003-07-14
Abstract
Abstract A method of recording a densitogram representing densities of albumin, α 1 -globulin, α 2 -globulin, β-globulin and γ-globulin in an electrophoresis is disclosed. In order to display the densitogram representing the change in density of the substances, an area of the densitogram surrounded by a densitogram curve and a base line is made proportional to a total density T which is measured separately by a colorimetric method. Successive density signals X n are adjusted by multiplying them with a density output ratio R which is derived in accordance with the following equation, ##EQU1## wherein K is a coefficient denoting an area on a record paper per unit density, ΣX n is an accumulated density signal, Y is a gain of a recorder and P is a recording pitch of the recorder.
Inventors
- 👤 Nobutaka Kaneko
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 13:43
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721; G01D; G01D9/00; G01D9/02
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
122 words
A method of recording a densitogram on a record medium with respect to a base line, said densitogram representing densities of a plurality of fractionated substances contained in a sample comprising the steps of: deriving density signals X n representing density values of the fractionated substances; processing the density signals in such a manner that an area S surround by a densitogram curve to be recorded on the record medium and the base line is made equal to a product of a coefficient K denoting an area on the record medium per unit density and a total density T of the pluarality of fractionated substances; and supplying the processed density signals to a recorder to form a densitogram on the record medium.
Claim 2
Independent Claim
122 words
A method of recording a densitogram on a record medium with respect to a base line, said densitogram representing densities of a plurality of fractionated substances contained in a sample comprising the steps of: deriving density signals X n representing density values of the fractionated substances; processing the density signals in such a manner that an area S surround by a densitogram curve to be recorded on the record medium and the base line is made equal to a product of a coefficient K denoting an area on the record medium per unit density and a total density T of the pluarality of fractionated substances; and supplying the processed density signals to a recorder to form a densitogram on the record medium.
Claim 3
Dependent Claim
91 words
A method according to claim 1, wherein said processing step comprises storing the density signals in a memory means; accumulating the successive density signals X n to produce an accumulated density value ΣX n ; setting values of the coefficint K, a gain Y of the recorder and a recording pitch P of the recorder; calculating a density output ratio R in accordance with an equation of ##EQU3## deriving products of respective density signals X n and the density output ratio R to produce the processed density signals R·X n .
Claim 4
Dependent Claim
22 words
A method according to claim 2, wherein said accumulating step comprises accumulating the successive density signals read out of the memory means.
Claim 5
Dependent Claim
39 words
A method according to claim 2, wherein said total density T is stored in a memory unit and said calculation for the density output ratio R is effect by reading the total density T out of the memory unit.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-03-10 | 1989-09-09 | 1990-03-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-03-10 | 1993-09-09 | 1994-03-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-03-10 | 1997-09-09 | 1998-03-11 | $7700.00 | Window Closed / Lapsed |