US4575452A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Kit for silver staining proteins and nucleic acids
Assignee: Revvity Health Sciences Inc
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Filing: 1984-09-21
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Granted: 1986-03-11
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Expires: 2004-09-21
Abstract
Abstract A method and kit for the optical detection of proteins and nucleic acids in a matrix, such as polyacrylamide electrophoresis gels. The method comprises fixing the proteins and nucleic acids in the matrix using aromatic sulfonic acids having tertiary amines capable of forming coordination complexes with silver ion.
Inventors
- 👤 Duk H. Lee
- 👤 Thomas J. O'Connell, III
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 15:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6827; G01N33/683; G01N33/6836; C; C12; C12Q; C12Q1/00; C12Q1/68; G01N1/00; G01N1/28; G01N1/30; G01N2001/305; Y; Y10; Y10S; Y10S436/00; Y10S436/905; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/25
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
97 words
A kit for the optical detection of proteins and nucleic acids in a matrix, comprising: (a) a fixer comprising a compound selected from the group consisting of ##STR16## wherein R is H, CH 3 , C 2 H 5 or CH 2 N + (CH 3 ) 3 , ##STR17## (b) a sensitizer selected from the group consisting of sodium sulfide, thiourea, dithiothreitol and sodium thiosulfate; (c) a source of silver ions; (d) a developer capable of reducing silver ions to metallic silver; and (e) a stopper capable of stopping reduction of silver ions to metallic silver.
Claim 2
Dependent Claim
97 words
A kit for the optical detection of proteins and nucleic acids in a matrix, comprising: (a) a fixer comprising a compound selected from the group consisting of ##STR16## wherein R is H, CH 3 , C 2 H 5 or CH 2 N + (CH 3 ) 3 , ##STR17## (b) a sensitizer selected from the group consisting of sodium sulfide, thiourea, dithiothreitol and sodium thiosulfate; (c) a source of silver ions; (d) a developer capable of reducing silver ions to metallic silver; and (e) a stopper capable of stopping reduction of silver ions to metallic silver.
Claim 3
Dependent Claim
13 words
The kit of claim 1 wherein the developer comprises sodium phosphate and formaldehyde.
Claim 4
Dependent Claim
11 words
The kit of claim 1 wherein the stopper comprises citric acid.
References Cited (Backward Citations)
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US4468466A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-03-10 | 1989-09-09 | 1990-03-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-03-10 | 1993-09-09 | 1994-03-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-03-10 | 1997-09-09 | 1998-03-11 | $7700.00 | Window Closed / Lapsed |