Patent US4574850A successfully refreshed from Google Patents.
US4574850A
Expired / Lapsed
US
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Method of and apparatus for dispensing liquid
Assignee: Dade Behring Inc
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Filing: 1985-01-17
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Granted: 1986-03-11
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Expires: 2005-01-17
Abstract
Abstract A liquid pipetting system for aspirating and dispensing small samples of fluid and diluents. The pipetting system consists of a coaxial needle serving as the pipette tip and a metering system to control the fluids. Precise dispensing of small amounts of liquid from the inner needle is made possible by surrounding the sample liquid with a miscible diluting liquid thereby establishing a controlled interface and conveying the sample liquid into the receiving chamber with that miscible diluting liquid.
Inventors
- 👤 James E. Davis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 22:56
CPC Classifications
G; G01; G01N; G01N35/00; G01N35/10; G01N1/00; G01N1/28; G01N1/38
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
187 words
A liquid pipetting system for aspirating and precisely dispensing small samples of a first liquid, having an outer conduit having outlet and supply ends; means connected to the supply end of the outer conduit for supplying a liquid thereto; an inner conduit having inlet and supply ends and coaxially disposed in the outer conduit to define an annular space therebetween; a sample pump means connected to the supply end of the inner conduit; means for immersing the inlet end of the inner conduit in the first liquid; and means for selectively actuating the sample pump means to aspirate the first liquid into the inlet end of the inner conduit; the system characterized by a diluent pump connected to the supply end of the outer conduit for supplying a diluent liquid miscible with the first liquid thereto, and means for dispensing a portion of the aspirated first liquid by selectively activating the pumps to dispense a portion of the first liquid from the inner conduit into the diluent liquid in the annular space and simultaneously to flush the inlet end of the inner conduit with the diluent liquid.
Claim 2
Dependent Claim
187 words
A liquid pipetting system for aspirating and precisely dispensing small samples of a first liquid, having an outer conduit having outlet and supply ends; means connected to the supply end of the outer conduit for supplying a liquid thereto; an inner conduit having inlet and supply ends and coaxially disposed in the outer conduit to define an annular space therebetween; a sample pump means connected to the supply end of the inner conduit; means for immersing the inlet end of the inner conduit in the first liquid; and means for selectively actuating the sample pump means to aspirate the first liquid into the inlet end of the inner conduit; the system characterized by a diluent pump connected to the supply end of the outer conduit for supplying a diluent liquid miscible with the first liquid thereto, and means for dispensing a portion of the aspirated first liquid by selectively activating the pumps to dispense a portion of the first liquid from the inner conduit into the diluent liquid in the annular space and simultaneously to flush the inlet end of the inner conduit with the diluent liquid.
Claim 3
Dependent Claim
24 words
A system as set forth in claim 1 wherein the outer conduit has an axis and the inner conduit is positionable along the axis.
Claim 4
Dependent Claim
25 words
A system as set forth in claim 2 wherein the inner conduit is slideably supported by protuberances in the inner wall of the outer conduit.
Claim 5
Dependent Claim
27 words
A system as set forth in claim 2 wherein the immersing means incudes means for selectively extending only the inner conduit into the first liquid for aspiration.
Claim 6
Dependent Claim
43 words
A system as set forth in claim 1 wherein the immersing means includes means for dipping the inlet ends of the conduits in the first liquid, and means to selectively activate the diluent pump to aspirate the first liquid to the annular space.
Claim 7
Dependent Claim
20 words
A system as set forth in claim 1 wherein the outer conduit is of reduced diameter at the inlet end.
Claim 8
Dependent Claim
20 words
A system as set forth in claim 1 wherein the inner conduit is of reduced diameter at the inlet end.
Claim 9
Dependent Claim
19 words
A system as set forth in claim 1 wherein both conduits are of reduced diameter at their inlet ends.
Claim 10
Independent Claim
118 words
A method for accurately aspirating and dispensing small samples of a first liquid using a probe having inner and outer coaxially disposed conduits defining a coaxial region comprising the steps of: aspirating the first liquid into the inner conduit; flushing the annular space between the conduits with a second liquid miscible with the first liquid to remove the first liquid from the entire exterior of the inner conduit and establish a controlled interface between the first and second liquids; dispensing a predetermined quantity of the first liquid from the inner conduit into the second liquid in the annular space; and flushing the annular space with a predetermined quantity of the second liquid thereby precisely dispensing the first liquid.
Claim 11
Independent Claim
118 words
A method for accurately aspirating and dispensing small samples of a first liquid using a probe having inner and outer coaxially disposed conduits defining a coaxial region comprising the steps of: aspirating the first liquid into the inner conduit; flushing the annular space between the conduits with a second liquid miscible with the first liquid to remove the first liquid from the entire exterior of the inner conduit and establish a controlled interface between the first and second liquids; dispensing a predetermined quantity of the first liquid from the inner conduit into the second liquid in the annular space; and flushing the annular space with a predetermined quantity of the second liquid thereby precisely dispensing the first liquid.
Claim 12
Dependent Claim
48 words
A method set forth in claim 9 which includes the additional step of contacting the tip of the outer probe with a surface to remove excess liquid prior to dispensing and flushing; and initially aspirating the first liquid into the annular space as well as the inner conduit.
Claim 13
Dependent Claim
36 words
A method as set forth in claim 9 wherein the inner conduit alone is extended axially for immersion in the first liquid prior to aspiration, and withdrawn into the outer conduit prior to the initial flushing.
Claim 14
Dependent Claim
32 words
A method as set forth in claim 11 which includes the additional step of contacting the tip of the probe with a surface to remove excess liquid prior to dispensing and flushing.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-03-10 | 1989-09-09 | 1990-03-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-03-10 | 1993-09-09 | 1994-03-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-03-10 | 1997-09-09 | 1998-03-11 | $7700.00 | Window Closed / Lapsed |