Patent US4572901A successfully refreshed from Google Patents.
US4572901A
Expired / Lapsed
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Method and composition for protein immobilization
Assignee: UCSF Benioff Children's Hospital
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Filing: 1983-06-23
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Granted: 1986-02-25
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Expires: 2003-06-23
Abstract
Abstract Compositions, articles of manufacture, and methods are provided for enhanced binding of compounds to a surface. The compositions find particular use in coating wells, slides, other surfaces, where ligands or receptors are to be bound and detrimental non-specific binding may be encountered. Particularly, proteins are modified by alkylation to produce a product which strongly adheres to a surface, allows ligands and receptors to be firmly attached by means of the alkylated protein to a surface under mild non-denaturing conditions and permits procedures to minimize non-specific binding of proteins without significant loss of the materials of interest.
Inventors
- 👤 Roberto L. Ceriani
- 👤 Jerry A. Peterson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6842; G01N33/53; G01N33/543; G01N33/54393; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/823
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
139 words
In a method for carrying out a diagnostic assay, wherein at least one of the analyte and reagent are a poly(amino acid), and the analyte is a member of a specific binding pair consisting of ligand and receptor, wherein one of said specific pair is bound to a solid surface, and the analyte is determined by the binding of analyte to its complementary member bound to the solid support, the improvement which comprises employing as a support a solid support coated with an alkylated poly(amino acid) of at least about 30,000 molecular weight, wherein said alkyl groups are from one to three carbon atoms, said alkylated poly(amino acid) being insoluble in water at 25° C. and soluble to at least 0.01 weight percent in an aqueous solution of a non-ionic detergent present in at least about 0.005 weight percent.
Claim 2
Dependent Claim
139 words
In a method for carrying out a diagnostic assay, wherein at least one of the analyte and reagent are a poly(amino acid), and the analyte is a member of a specific binding pair consisting of ligand and receptor, wherein one of said specific pair is bound to a solid surface, and the analyte is determined by the binding of analyte to its complementary member bound to the solid support, the improvement which comprises employing as a support a solid support coated with an alkylated poly(amino acid) of at least about 30,000 molecular weight, wherein said alkyl groups are from one to three carbon atoms, said alkylated poly(amino acid) being insoluble in water at 25° C. and soluble to at least 0.01 weight percent in an aqueous solution of a non-ionic detergent present in at least about 0.005 weight percent.
Claim 3
Dependent Claim
18 words
A method according to claim 1, wherein a ligand or receptor is bound to said alkylated poly(amino acid).
Claim 4
Dependent Claim
11 words
A method according to claim 2, wherein said alkyl is methyl.
Claim 5
Dependent Claim
24 words
A method according to claim 3, wherein said alkylated poly(amino acid) is methylated bovine serum albumin, which is methylated employing absolute methanol and HCl.
Claim 6
Dependent Claim
67 words
An article of manufacture comprising a solid support coated with an alkylated poly(amino acid) of at least about 30,000 molecular weight, wherein said alkyl groups are from one to three carbon atoms, said alkylated poly(amino acid) being insoluble in water at 25° C. and soluble to at least 0.01 weight percent in an aqueous solution of a non-ionic detergent present in at least about 0.005 weight percent.
Claim 7
Dependent Claim
67 words
An article of manufacture comprising a solid support coated with an alkylated poly(amino acid) of at least about 30,000 molecular weight, wherein said alkyl groups are from one to three carbon atoms, said alkylated poly(amino acid) being insoluble in water at 25° C. and soluble to at least 0.01 weight percent in an aqueous solution of a non-ionic detergent present in at least about 0.005 weight percent.
Claim 8
Dependent Claim
26 words
An article of manufacture according to claim 5, wherein said alkyl is methyl and said non-ionic detergent is present in about 0.01% and is Triton X-100.
Claim 9
Dependent Claim
36 words
An article of manufacture according to claim 5, wherein said alkyl is methyl and methylation is performed with absolute methanol and HCl at moderate temperatures to alkylate at least about 40% of the available carbonyl groups.
Claim 10
Dependent Claim
20 words
An article of manufacture according to claim 5, wherein a ligand or a receptor is bound to said poly(amino acid).
Claim 11
Dependent Claim
16 words
An article of manufacture according to claim 5, wherein said poly(amino acid) is bovine serum albumin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-02-24 | 1989-08-26 | 1990-02-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-02-24 | 1993-08-26 | 1994-02-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-02-24 | 1997-08-26 | 1998-02-25 | $7700.00 | Window Closed / Lapsed |