US4571390A
Expired / Lapsed
US
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Substrate capable of adsorbing protein
Assignee: Kureha Corp
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Filing: 1984-05-08
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Granted: 1986-02-18
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Expires: 2004-05-08
Abstract
Abstract Disclosed herein are a porous substrate capable of adsorbing protein, having an exclusive molecular weight of at least 30,000, comprising a cross-linked copolymer having methylol groups, the copolymer being made of a monomer of styrenes and a cross-linking agent copolymerizable with the monomer, the outer surface of the porous substrate being hydrophilic and the pore surface thereof being hydrophobic, and a process for producing the same.
Inventors
- 👤 Teruo Sakagami
- 👤 Masuhiro Shoji
- 👤 Noriyuki Arakawa
- 👤 Naohiro Murayama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 06:52
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01J; B01J20/00; B01J20/22; B01J20/26; B01J20/265; B01J20/267; B01J20/281; B01J20/282; B01J20/285; C; C08; C08F; C08F8/00; C08F8/26; C08J; C08J9/00; C08J9/28; C08J9/286; B01D15/26; B01D15/38; B01D15/3804; B01J2220/00; B01J2220/50; B01J2220/54; C08F2810/00; C08F2810/20
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
111 words
A porous substrate capable of absorbing protein, having an exclusive molecular weight of at least 30,000 having a hydrophilic outer surface and a hydrophobic pore surface which substrate comprises a cross-linked copolymer having methylol groups, produced by subjecting a monomeric mixture of at least one monomer selected from styrene, alpha-methylstyrene and chloromethylstyrenes and a cross-linking agent copolymerizable with the monomer to suspension copolymerization in an aqueous solution of 5 to 60 parts by weight of the water-soluble high polymeric substance per 100 parts by weight of the monomeric mixture of the monomer and the cross-linking agent in the presence of a pore-regulator, and introducing methylol groups into the resultant cross-linked copolymer.
Claim 2
Dependent Claim
111 words
A porous substrate capable of absorbing protein, having an exclusive molecular weight of at least 30,000 having a hydrophilic outer surface and a hydrophobic pore surface which substrate comprises a cross-linked copolymer having methylol groups, produced by subjecting a monomeric mixture of at least one monomer selected from styrene, alpha-methylstyrene and chloromethylstyrenes and a cross-linking agent copolymerizable with the monomer to suspension copolymerization in an aqueous solution of 5 to 60 parts by weight of the water-soluble high polymeric substance per 100 parts by weight of the monomeric mixture of the monomer and the cross-linking agent in the presence of a pore-regulator, and introducing methylol groups into the resultant cross-linked copolymer.
Claim 3
Dependent Claim
30 words
The substrate of claim 1, in which the cross-linking agent is selected from the group consisting of divinylbenzene, trivinylbenzene, triallyl isocyanurate, dimethacrylates and diacrylates of polyhydric alcohols and diallyl phthalate.
Claim 4
Dependent Claim
14 words
The substrate of claim 1, in which the cross-linked copolymer is a styrene-divinylbenzene copolymer.
Claim 5
Dependent Claim
20 words
The substrate of claim 1, which is a spherical particle having a diameter in the range of 1 to 300μ.
Claim 6
Dependent Claim
15 words
The substrate of claim 1, which has an exclusive molecular weight of at least 50,000.
Claim 7
Dependent Claim
16 words
The substrate of claim 1, which has at least 0.1 methylol group per one aromatic moiety.
Claim 8
Dependent Claim
98 words
A process for preparing a porous substrate capable of adsorbing protein, comprising (1) subjecting a mixture of a monomer of styrenes and a cross-linking agent copolymerizable with the monomer to suspension polymerization in an aqueous solution of 5 to 60 parts by weight of the water-soluble high polymeric substance per 100 parts by weight of the monomeric mixture of the monomer and the cross-linking agent in the presence of a pore regulator, said pore regulator providing the porous substrate with an exclusive molecular weight of at least 30,000, and (2) introducing methylol groups into the resultant cross-linked polymer.
Claim 9
Dependent Claim
98 words
A process for preparing a porous substrate capable of adsorbing protein, comprising (1) subjecting a mixture of a monomer of styrenes and a cross-linking agent copolymerizable with the monomer to suspension polymerization in an aqueous solution of 5 to 60 parts by weight of the water-soluble high polymeric substance per 100 parts by weight of the monomeric mixture of the monomer and the cross-linking agent in the presence of a pore regulator, said pore regulator providing the porous substrate with an exclusive molecular weight of at least 30,000, and (2) introducing methylol groups into the resultant cross-linked polymer.
Claim 10
Dependent Claim
24 words
The process of claim 7, wherein the monomer of styrenes is selected from the group consisting of styrene, α-methylstyrene, chloromethylstyrene and a mixture thereof.
Claim 11
Dependent Claim
29 words
The process of claim 7 wherein the cross-linking agent is selected from the group consisting of divinylbenzene, trivinylbenzene, triallyl isocyanurate, dimethacrylates and diacrylates of polyhydric alcohols and diallyl phthalate.
Claim 12
Dependent Claim
16 words
The process of claim 7, wherein the monomer is styrene and the cross-linking agent is divinylbenzene.
Claim 13
Dependent Claim
36 words
The process of claim 7, wherein the amount of the cross-linking agent is in the range of 30 to 70% by weight to the total amount of the mixture of the monomer and the cross-linking agent.
Claim 14
Dependent Claim
30 words
The process of claim 7, wherein the water-soluble high polymeric substance is selected from the group consisting of polyethylene oxide, saponified polyvinyl acetates, polyvinyl alcohol, polyvinyl pyrrolidone and methyl cellulose.
Claim 15
Dependent Claim
40 words
The process of claim 7, wherein the amount of the water-soluble high polymeric substance is in the range of 10 to 40 parts by weight per 100 parts by weight of the mixture of the monomer and the cross-linking agent.
Claim 16
Dependent Claim
16 words
The process of claim 7, wherein the pore regulator is organic solvent soluble in the monomer.
Claim 17
Dependent Claim
28 words
The process of claim 14, wherein the pore regulator is selected from the group consisting of an aromatic hydrocarbon, a chlorohydrocarbon, an aliphatic hydrocarbon and a mixture thereof.
Claim 18
Dependent Claim
32 words
The process of claim 15, wherein the aromatic hydrocarbon is benzene, tolouene, or xylene, the chlorohydrocarbon is trichloroethylene, chloroform, or carbon tetrachloride, and the aliphatic hydrocarbon is n-hexane, n-heptane, n-octane, or n-dodecane.
Claim 19
Dependent Claim
38 words
The process of claim 7, wherein the amount of the pore regulator is in the range of 20 to 300 parts by weight of 100 parts by weight of the mixture of the monomer and the cross-linking agent.
Claim 20
Dependent Claim
22 words
The process of claim 7, wherein the resultant cross-linked copolymer is provided with methylol groups by chloromethylating the copolymer followed by hydrolysis.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-02-17 | 1989-08-19 | 1990-02-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-02-17 | 1993-08-19 | 1994-02-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-02-17 | 1997-08-19 | 1998-02-18 | $7700.00 | Window Closed / Lapsed |