Patent US4567139A successfully refreshed from Google Patents.
US4567139A
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Chromogen for measuring the formation of hydrogen peroxide based on 1-substituted-aminoantipyrin compounds
Assignee: Roche Diagnostics GmbH
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Filing: 1983-02-24
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Granted: 1986-01-28
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Expires: 2003-02-24
Abstract
Abstract Compounds of the general formula <IMAGE> wherein R represents an alkyl group of 1 to 3 carbon atoms or the group N(R2)2, in which R2 represents a hydrogen atom, an alkyl group of 1 to 3 carbon atoms, or an acyl group of 1 to 3 carbon atoms, and R1 has the same meaning as R or is a hydrogen atom, are new and are suitable as chromogens for measuring H2O2 formation in enzymatic reactions.
Inventors
- 👤 Hans-George Batz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:07
CPC Classifications
C; C07; C07D; C07D231/00; C07D231/02; C07D231/10; C07D231/14; C07D231/18; C07D231/20; C07D231/22; C07D231/26; C07D231/44; C07D231/46; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/50; C12Q2326/90; C12Q2326/96; Y; Y10; Y10S; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/20; Y10T436/206664
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
133 words
In a method for the determination of enzymatically formed hydrogen peroxide of the type wherein phenol or other phenolic compound is oxidatively coupled with 4-aminoantipyrine as the chromogen, in the presence of peroxidase, by the action of hydrogen peroxide, to form a dye which is determined photometrically, the improvement comprising replacing the 4-aminoantipyrine with a compound of the formula ##STR10## wherein R represents the group N(R 2 ) 2 , in which R 2 represents an alkyl group of 1 to 3 carbon atoms or one R 2 is an acyl group of 1 to 3 carbon atoms and the other R 2 is hydrogen or an alkyl group of 1 to 3 carbon atoms, and R 1 has the same meaning as R or is a hydrogen atom or NH 2 .
Claim 2
Dependent Claim
133 words
In a method for the determination of enzymatically formed hydrogen peroxide of the type wherein phenol or other phenolic compound is oxidatively coupled with 4-aminoantipyrine as the chromogen, in the presence of peroxidase, by the action of hydrogen peroxide, to form a dye which is determined photometrically, the improvement comprising replacing the 4-aminoantipyrine with a compound of the formula ##STR10## wherein R represents the group N(R 2 ) 2 , in which R 2 represents an alkyl group of 1 to 3 carbon atoms or one R 2 is an acyl group of 1 to 3 carbon atoms and the other R 2 is hydrogen or an alkyl group of 1 to 3 carbon atoms, and R 1 has the same meaning as R or is a hydrogen atom or NH 2 .
Claim 3
Dependent Claim
9 words
The method of claim 1 wherein R is NH-acetyl.
Claim 4
Dependent Claim
26 words
The method of claim 1 wherein R is N(R 2 ) 2 in which R 2 is an alkyl group of 1 to 3 carbon atoms.
Claim 5
Dependent Claim
10 words
The method of claim 1 wherein R 1 is hydrogen.
Claim 6
Dependent Claim
12 words
The method of claim 1 wherein R 1 is NH 2 .
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-01-27 | 1989-07-29 | 1990-01-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-01-27 | 1993-07-29 | 1994-01-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-01-27 | 1997-07-29 | 1998-01-28 | $7700.00 | Window Closed / Lapsed |