Patent US4558953A successfully refreshed from Google Patents.
US4558953A
Expired / Lapsed
US
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Colorimetric method and apparatus
Assignee: Olympus Corp
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Filing: 1982-11-24
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Granted: 1985-12-17
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Expires: 2002-11-24
Abstract
Abstract In a colorimeter for use in a biochemistry field, an amount of transmitted light impinging upon a light receiving device is decreased corresponding to an absorbance of a reagent to be used by arranging a light adjusting device in the optical path. The amount of light transmitted through a blank cuvette is made substantially equal to an amount of light transmitted through the reagent. Therefore, even when the analysis is performed for an item using a high absorbance reagent, the deviation of the amount of transmitted light does not affect measured absorbance values, and thus it is possible to perform the color measurement in an accurate manner regardless of the reagent absorbance.
Inventors
- 👤 Takashi Yamada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:22
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/255; G01J; G01J1/00; G01J1/10; G01J1/20; G01J1/22; G01J1/24; G01N21/75; G01N21/77; G01N21/78; G01N2201/00; G01N2201/12; G01N2201/127
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
263 words
A colorimetric method for effecting a quantitative analysis for specific substances contained in a sample comprising the steps of: placing a blank cuvette into an optical path between a light source emitting a light beam and a light receiving means for detecting said light beam; placing a light adjusting means between said blank cuvette and said light receiving means; selectively adjusting the amount of light transmitted through said blank cuvette to said light receiving device by adjusting said light adjusting means such that the amount of light detected by said light receiving means approximates the amount of light which will be detected by said light receiving means when absorbance of a reagent to be used is measured in said cuvette when said light adjusting means is set to allow the passage of the maximum amount of light to said light receiving means; effecting a 100% adjustment of said transmitted light, i.e., a zero absorbance adjustment for said blank cuvette when said adjusted light is detected by said light receiving means; supplying said reagent to said cuvette; measuring the absorbance of said reagent while said light adjusting means allows the maximum amount of light to pass to said light receiving means; supplying a sample to said cuvette, and reacting said sample with said reagent; measuring the absorbance of said reacted sample while said light adjusting means allows the maximum amount of light to pass to said light receiving means; and obtaining the difference between said absorbance of said reagent and said absorbance of said reacted sample to derive the absorbance value for said sample.
Claim 2
Dependent Claim
263 words
A colorimetric method for effecting a quantitative analysis for specific substances contained in a sample comprising the steps of: placing a blank cuvette into an optical path between a light source emitting a light beam and a light receiving means for detecting said light beam; placing a light adjusting means between said blank cuvette and said light receiving means; selectively adjusting the amount of light transmitted through said blank cuvette to said light receiving device by adjusting said light adjusting means such that the amount of light detected by said light receiving means approximates the amount of light which will be detected by said light receiving means when absorbance of a reagent to be used is measured in said cuvette when said light adjusting means is set to allow the passage of the maximum amount of light to said light receiving means; effecting a 100% adjustment of said transmitted light, i.e., a zero absorbance adjustment for said blank cuvette when said adjusted light is detected by said light receiving means; supplying said reagent to said cuvette; measuring the absorbance of said reagent while said light adjusting means allows the maximum amount of light to pass to said light receiving means; supplying a sample to said cuvette, and reacting said sample with said reagent; measuring the absorbance of said reacted sample while said light adjusting means allows the maximum amount of light to pass to said light receiving means; and obtaining the difference between said absorbance of said reagent and said absorbance of said reacted sample to derive the absorbance value for said sample.
Claim 3
Dependent Claim
42 words
The colorimetric method according to claim 1, wherein said adjusted light for effectuating the zero absorbance adjustment is determined to be slightly larger than the amount of light detected by said light receiving means when the absorbance of the reagent is measured.
Claim 4
Dependent Claim
44 words
The colorimetric method according to claim 2, wherein said adjusted light for effectuating the zero absorbance adjustment is determined to be slightly larger than the amount of light detected by said light receiving means when the absorbance of the reacted sample solution is measured.
Claim 5
Dependent Claim
234 words
A colorimeter comprising: a light source emitting a light beam onto a cuvette; a light receiving means for detecting the light beam transmitted through said cuvette, said cuvette positioned between said light source and said light receiving means; and a light adjusting means arranged in an optical path between said light source and said light receiving device for selectively adjusting the amount of light transmitted through a blank cuvette to said light receiving device by adjusting said light adjusting means such that the amount of light transmitted through said blank cuvette and detected by said light receiving means approximates the amount of light which will be detected by said light receiving means when the absorbance of a reagent to be used is measured in said cuvette when said light adjusting means is set to allow the passage of the maximum amount of light to said light receiving means; a means to measure the absorbance of said reagent while said light adjusting means allows the maximum amount of light to pass to said light receiving means; a means to measure the absorbance of a reacted sample in said cuvette while said light adjusting means allows the maximum amount of light to pass to said light receiving means; and a means to obtain the difference between said absorbance of said reagent and said absorbance of said reacted sample to derive the absorbance value for said sample.
Claim 6
Dependent Claim
234 words
A colorimeter comprising: a light source emitting a light beam onto a cuvette; a light receiving means for detecting the light beam transmitted through said cuvette, said cuvette positioned between said light source and said light receiving means; and a light adjusting means arranged in an optical path between said light source and said light receiving device for selectively adjusting the amount of light transmitted through a blank cuvette to said light receiving device by adjusting said light adjusting means such that the amount of light transmitted through said blank cuvette and detected by said light receiving means approximates the amount of light which will be detected by said light receiving means when the absorbance of a reagent to be used is measured in said cuvette when said light adjusting means is set to allow the passage of the maximum amount of light to said light receiving means; a means to measure the absorbance of said reagent while said light adjusting means allows the maximum amount of light to pass to said light receiving means; a means to measure the absorbance of a reacted sample in said cuvette while said light adjusting means allows the maximum amount of light to pass to said light receiving means; and a means to obtain the difference between said absorbance of said reagent and said absorbance of said reacted sample to derive the absorbance value for said sample.
Claim 7
Dependent Claim
21 words
The colorimeter according to claim 4, wherein said light adjusting means is arranged between said cuvette and said light receiving means.
Claim 8
Dependent Claim
46 words
The colorimeter according to claim 5, wherein said light amount adjusting means comprises a rotary disc and a plurality of apertures having different transmissivities and formed in said disc along a circle, whereby any one of the apertures can be selectively inserted into said optical path.
Claim 9
Dependent Claim
45 words
The colorimeter according to claim 5, wherein said light adjusting means comprises a rotary disc and plurality of N.D. filters having different transmissivities and arranged in said disc along a circle, whereby any one of the filters can be selectively inserted into said optical path.
Claim 10
Dependent Claim
25 words
The colorimeter according to claim 6, wherein said different transmissivities of said light adjusting means are set to 100%, 63.1%, 39.8%, 25.1%, 15.8% and 10.0%.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-12-16 | 1989-06-17 | 1989-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-12-16 | 1993-06-17 | 1993-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-12-16 | 1997-06-17 | 1997-12-17 | $7700.00 | Window Closed / Lapsed |