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US4552760A
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Method for stabilizing tissue plasminogen activator and a stable aqueous solution or powder containing the same
Assignee: Asahi Kasei Corp
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Filing: 1984-04-23
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Granted: 1985-11-12
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Expires: 2004-04-23
Abstract
Abstract A method for stabilizing a tissue plasminogen activator which involves adding to an aqueous solution or powder containing a tissue plasminogen activator an effective amount of a purified gelatin is disclosed. A stable aqueous solution or powder which contains a tissue plasminogen activator and an effective amount of a purified gelatin is also disclosed. The method and composition eliminate the problem of adsorption of tissue plasminogen activator to various laboratory equipment, and also prevent the conversion of the single-chain tissue plasminogen activator into a double-chain tissue plasminogen activator.
Inventors
- 👤 Akira Murakami
- 👤 Hideo Yoshizaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 16:30
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6459; A; A61; A61K; A61K47/00; A61K47/30; A61K47/42; C12N9/96; C12Y; C12Y304/00; C12Y304/21; C12Y304/21069; A61K38/00; G; G03; G03C; G03C1/00; G03C1/005; G03C1/04; G03C1/047; G03C2001/0471; Y; Y10; Y10S; Y10S530/00; Y10S530/808; Y10S530/827; Y10S530/848
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
37 words
A method for stabilizing a tissue plasminogen activator which comprises adding to an aqueous solution or powder containing a tissue plasminogen activator an effective amount of a purified gelatin having an isoelectric point of 7.0 to 9.0.
Claim 2
Independent Claim
37 words
A method for stabilizing a tissue plasminogen activator which comprises adding to an aqueous solution or powder containing a tissue plasminogen activator an effective amount of a purified gelatin having an isoelectric point of 7.0 to 9.0.
Claim 3
Dependent Claim
19 words
A method of claim 1, wherein the purified gelatin has a weight average molecular weight of 3,000 to 50,000.
Claim 4
Dependent Claim
19 words
A method of claim 2, wherein the purified gelatin has a weight average molecular weight of 4,000 to 20,000.
Claim 5
Dependent Claim
36 words
A method of claim 1, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 6
Dependent Claim
36 words
A method of claim 2, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 7
Dependent Claim
36 words
A method of claim 3, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 8
Dependent Claim
21 words
A method of claim 1, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 9
Dependent Claim
21 words
A method of claim 2, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 10
Dependent Claim
21 words
A method of claim 3, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 11
Dependent Claim
21 words
A method of claim 4, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 12
Dependent Claim
21 words
A method of claim 5, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 13
Dependent Claim
21 words
A method of claim 6, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 14
Dependent Claim
28 words
A stable aqueous solution or powder which contains a tissue plasminogen activator and an effective amount of a purified gelatin having an isoelectric point of 7.0 to 9.0.
Claim 15
Dependent Claim
28 words
A stable aqueous solution or powder which contains a tissue plasminogen activator and an effective amount of a purified gelatin having an isoelectric point of 7.0 to 9.0.
Claim 16
Dependent Claim
23 words
A stable aqueous solution or powder of claim 13, wherein the purified gelatin has a weight average molecular weight of 3,000 to 50,000.
Claim 17
Dependent Claim
23 words
A stable aqueous solution or powder of claim 14, wherein the purified gelatin has a weight average molecular weight of 4,000 to 20,000.
Claim 18
Dependent Claim
40 words
A stable aqueous solution or powder of claim 13, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 19
Dependent Claim
40 words
A stable aqueous solution or powder of claim 15, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 20
Dependent Claim
39 words
A stable aqueous solution or powder or claim 17, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator of 10 to 50,000 units/ml.
Claim 21
Dependent Claim
16 words
A method of claim 1 wherein the tissue plasminogen activator has been obtained from human tissue.
Claim 22
Dependent Claim
20 words
A stable aqueous solution or powder of claim 13 wherein the tissue plasminogen activator has been obtained from human tissue.
References Cited (Backward Citations)
US2930736A
Cited
US3322632A
Cited
US3296094A
Cited
US3998947A
Cited
US4083961A
Cited
US4151161A
Cited
US4245051A
Cited
US4264493A
Cited
US4286063A
Cited
US4317882A
Cited
US4495285A
Cited
US4495285B1
Cited
GB2119804A
Cited
JPS58224687A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-11-11 | 1989-05-13 | 1989-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-11-11 | 1993-05-13 | 1993-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-11-11 | 1997-05-13 | 1997-11-12 | $7700.00 | Window Closed / Lapsed |