US4552760A
Expired / Lapsed
US
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Method for stabilizing tissue plasminogen activator and a stable aqueous solution or powder containing the same
Assignee: Asahi Kasei Corp
•
Filing: 1984-04-23
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Granted: 1985-11-12
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Expires: 2004-04-23
Abstract
Abstract A method for stabilizing a tissue plasminogen activator which involves adding to an aqueous solution or powder containing a tissue plasminogen activator an effective amount of a purified gelatin is disclosed. A stable aqueous solution or powder which contains a tissue plasminogen activator and an effective amount of a purified gelatin is also disclosed. The method and composition eliminate the problem of adsorption of tissue plasminogen activator to various laboratory equipment, and also prevent the conversion of the single-chain tissue plasminogen activator into a double-chain tissue plasminogen activator.
Inventors
- 👤 Akira Murakami
- 👤 Hideo Yoshizaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 16:12
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6459; A; A61; A61K; A61K47/00; A61K47/30; A61K47/42; C12N9/96; C12Y; C12Y304/00; C12Y304/21; C12Y304/21069; A61K38/00; G; G03; G03C; G03C1/00; G03C1/005; G03C1/04; G03C1/047; G03C2001/0471; Y; Y10; Y10S; Y10S530/00; Y10S530/808; Y10S530/827; Y10S530/848
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
37 words
A method for stabilizing a tissue plasminogen activator which comprises adding to an aqueous solution or powder containing a tissue plasminogen activator an effective amount of a purified gelatin having an isoelectric point of 7.0 to 9.0.
Claim 2
Independent Claim
37 words
A method for stabilizing a tissue plasminogen activator which comprises adding to an aqueous solution or powder containing a tissue plasminogen activator an effective amount of a purified gelatin having an isoelectric point of 7.0 to 9.0.
Claim 3
Dependent Claim
19 words
A method of claim 1, wherein the purified gelatin has a weight average molecular weight of 3,000 to 50,000.
Claim 4
Dependent Claim
19 words
A method of claim 2, wherein the purified gelatin has a weight average molecular weight of 4,000 to 20,000.
Claim 5
Dependent Claim
36 words
A method of claim 1, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 6
Dependent Claim
36 words
A method of claim 2, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 7
Dependent Claim
36 words
A method of claim 3, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 8
Dependent Claim
21 words
A method of claim 1, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 9
Dependent Claim
21 words
A method of claim 2, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 10
Dependent Claim
21 words
A method of claim 3, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 11
Dependent Claim
21 words
A method of claim 4, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 12
Dependent Claim
21 words
A method of claim 5, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 13
Dependent Claim
21 words
A method of claim 6, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 14
Dependent Claim
28 words
A stable aqueous solution or powder which contains a tissue plasminogen activator and an effective amount of a purified gelatin having an isoelectric point of 7.0 to 9.0.
Claim 15
Dependent Claim
28 words
A stable aqueous solution or powder which contains a tissue plasminogen activator and an effective amount of a purified gelatin having an isoelectric point of 7.0 to 9.0.
Claim 16
Dependent Claim
23 words
A stable aqueous solution or powder of claim 13, wherein the purified gelatin has a weight average molecular weight of 3,000 to 50,000.
Claim 17
Dependent Claim
23 words
A stable aqueous solution or powder of claim 14, wherein the purified gelatin has a weight average molecular weight of 4,000 to 20,000.
Claim 18
Dependent Claim
40 words
A stable aqueous solution or powder of claim 13, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 19
Dependent Claim
40 words
A stable aqueous solution or powder of claim 15, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator activity of 10 to 50,000 units/ml.
Claim 20
Dependent Claim
39 words
A stable aqueous solution or powder or claim 17, wherein the purified gelatin is added in an amount of 0.0001 to 0.10 g per ml of the aqueous solution having a tissue plasminogen activator of 10 to 50,000 units/ml.
Claim 21
Dependent Claim
16 words
A method of claim 1 wherein the tissue plasminogen activator has been obtained from human tissue.
Claim 22
Dependent Claim
20 words
A stable aqueous solution or powder of claim 13 wherein the tissue plasminogen activator has been obtained from human tissue.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-11-11 | 1989-05-13 | 1989-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-11-11 | 1993-05-13 | 1993-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-11-11 | 1997-05-13 | 1997-11-12 | $7700.00 | Window Closed / Lapsed |