Patent US4544629A successfully refreshed from Google Patents.
US4544629A
Expired / Lapsed
US
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Receptor-based histamine assay
Assignee: 3M Co
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Filing: 1982-11-19
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Granted: 1985-10-01
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Expires: 2002-11-19
Abstract
Abstract A receptor-based clinical or research competitive inhibition assay method for determining histamine in body or laboratory fluids is disclosed. A kit for carrying out the method is also described comprising an amount of histamine receptors sufficient to provide an excess of receptors for binding with the amount of histamine in the sample to be assayed, and an amount of a histamine-indicator conjugate sufficient to react with the resulting unbound histamine receptors present after reaction of the histamine sample with the histamine receptors.
Inventors
- 👤 Thomas K. Rice
- 👤 Therese A. Senta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 17:38
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/811
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
196 words
A method for determining the amount of histamine in a liquid test sample using histamine receptors and a histamine-indicator conjugate comprising the steps of: a. contacting a measured amount of said test sample with an excess of substantially Purified histamine receptors which are free in solution or bound to a continuous solid phase support, b. incubating and reacting said test sample with said histamine receptors, which reaction leaves unbound histamine receptors available for further bonding, c. contacting an amount of histamine-indicator conjugate with the bound and unbound histamine receptors sufficient to bind said conjugate to all unbound histamine receptors, d. incubating and reacting said histamine-indicator conjugate with said unbound histamine receptors, e. removing unbound histamine-indicator conjugate if necessary to measure the amount of histamine-indicator conjugate bound to said histamine receptors, f. measuring the amount of histamine-indicator conjugate bound to the histamine receptors, and g. determining the amount of histamine in said test sample by comparison with standard curves, with the proviso that said histamine-indicator conjugate maintains its activity or undergoes stimulation of activity when bound to said histamine receptors, and said method being capable of measuring at least 0.05 ng of histamine in a test sample.
Claim 2
Independent Claim
196 words
A method for determining the amount of histamine in a liquid test sample using histamine receptors and a histamine-indicator conjugate comprising the steps of: a. contacting a measured amount of said test sample with an excess of substantially Purified histamine receptors which are free in solution or bound to a continuous solid phase support, b. incubating and reacting said test sample with said histamine receptors, which reaction leaves unbound histamine receptors available for further bonding, c. contacting an amount of histamine-indicator conjugate with the bound and unbound histamine receptors sufficient to bind said conjugate to all unbound histamine receptors, d. incubating and reacting said histamine-indicator conjugate with said unbound histamine receptors, e. removing unbound histamine-indicator conjugate if necessary to measure the amount of histamine-indicator conjugate bound to said histamine receptors, f. measuring the amount of histamine-indicator conjugate bound to the histamine receptors, and g. determining the amount of histamine in said test sample by comparison with standard curves, with the proviso that said histamine-indicator conjugate maintains its activity or undergoes stimulation of activity when bound to said histamine receptors, and said method being capable of measuring at least 0.05 ng of histamine in a test sample.
Claim 3
Dependent Claim
23 words
The method according to claim 1 wherein said test sample and said histamine-indicator conjugate are simultaneously contacted and incubated with said histamine receptors.
Claim 4
Dependent Claim
14 words
The method according to claim 1 wherein said histamine-indicator conjugate is a histamine-enzyme conjugate.
Claim 5
Dependent Claim
19 words
The method according to claim 3 wherein the indicator of said histamine-indicator conjugate is alkaline phosphatase or horseradish peroxidase.
Claim 6
Dependent Claim
15 words
The method according to claim 1 wherein the source of histamine receptors is mammalian cells.
Claim 7
Dependent Claim
18 words
The method according to claim 1 wherein the source of histamine receptors is human lymphoid cells in culture.
Claim 8
Dependent Claim
18 words
The method according to claim 1 wherein the source of histamine receptors is lymphoid cells grown in suspension.
Claim 9
Dependent Claim
22 words
The method according to claim 1 wherein the source of histamine receptors is mammalian and the histamine-indicator conjugate is a histamine-enzyme conjugate.
Claim 10
Dependent Claim
24 words
The method according to claim 1 wherein more than 5 percent but less than 95 percent of the receptors have been reacted with histamine.
Claim 11
Dependent Claim
26 words
The method according to claim 1 wherein less than 95 percent but more than 5 percent of the receptors have been reacted with a histamine-indicator conjugate.
Claim 12
Dependent Claim
14 words
The method according to claim 1 wherein said histamine receptors are bound to cells.
Claim 13
Dependent Claim
111 words
A kit for use in the histamine assay method of claim 1 comprising (a) substantially purified histamine receptors which are free in solution or bound to a continuous solid phase support in an amount in excess of the amount required to bind all of the histamine in a test sample, and (b) a histamine-indicator conjugate in an amount sufficient to react with all unbound histamine receptors remaining after reaction of the test sample with said histamine receptors, said histamine-indicator conjugate maintains its activity or undergoes stimulation of activity when bound to said histamine receptors, and said kit being capable of measuring at least 0.05 ng of histamine in a test sample.
Claim 14
Dependent Claim
32 words
The kit according to claim 12 further comprising histamine standards to be admixed with a portion of said histamine receptors and subsequently or simultaneously with a portion of said histamine indicator conjugate.
Claim 15
Dependent Claim
35 words
The kit according to claim 12 useful in an enzyme stimulation assay method for the determination of histamine wherein said receptors are free in solution or are bound to (a) cells or (b) solid surfaces.
Claim 16
Dependent Claim
36 words
The kit according to claim 12 further comprising an amount of an allergen sufficient to stimulate histamine release from basophils or mast cells from an allergically sensitive mammal, said allergen being incorporated with said histamine receptors.
Claim 17
Independent Claim
195 words
A method for determining the amount of histamine in a liquid test sample using histamine receptors and histamine-alkaline phosphates as a histamine-indicator conjugate comprising the steps of: a. contacting a measured amount of said test sample with an excess of substantially purified histamine receptors which are free in solution or bound to a continuous solid phase support, b. incubating and reacting said test sample with said histamine receptors, which reaction leaves unbound histamine receptors available for further bonding, c. contacting an amount of histamine-alkaline phosphatase with the bound and unbound histamine receptors sufficient to bind said conjugate to all unbound histamine receptors, d. incubating and reacting said histamine-alkaline phosphatase with said unbound histamine receptors, e. removing unbound histamine-alkaline phosphatase if necessary to measure the amount of histamine-alkaline phosphatase bound to said histamine receptors, f. measuring the amount of histamine-alkaline phosphatase bound to the histamine receptors, and g. determining the amount of histamine in said test sample by comparison with standard curves, with the proviso that said histamine-alkaline phosphatase undergoes stimulation of activity when bound to said histamine receptors, and said method being capable of measuring at least 0.05 ng of histamine in a test sample.
Claim 18
Independent Claim
195 words
A method for determining the amount of histamine in a liquid test sample using histamine receptors and histamine-alkaline phosphates as a histamine-indicator conjugate comprising the steps of: a. contacting a measured amount of said test sample with an excess of substantially purified histamine receptors which are free in solution or bound to a continuous solid phase support, b. incubating and reacting said test sample with said histamine receptors, which reaction leaves unbound histamine receptors available for further bonding, c. contacting an amount of histamine-alkaline phosphatase with the bound and unbound histamine receptors sufficient to bind said conjugate to all unbound histamine receptors, d. incubating and reacting said histamine-alkaline phosphatase with said unbound histamine receptors, e. removing unbound histamine-alkaline phosphatase if necessary to measure the amount of histamine-alkaline phosphatase bound to said histamine receptors, f. measuring the amount of histamine-alkaline phosphatase bound to the histamine receptors, and g. determining the amount of histamine in said test sample by comparison with standard curves, with the proviso that said histamine-alkaline phosphatase undergoes stimulation of activity when bound to said histamine receptors, and said method being capable of measuring at least 0.05 ng of histamine in a test sample.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1988-09-30 | 1989-04-01 | 1989-10-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-30 | 1993-04-01 | 1993-10-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-09-30 | 1997-04-01 | 1997-10-01 | $7700.00 | Window Closed / Lapsed |