US4541952A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purification method of human interferon
Assignee: Toray Industries Inc
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Filing: 1983-05-12
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Granted: 1985-09-17
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Expires: 2003-05-12
Abstract
Abstract A method for the purification of human interferon by the successive treatments of a crude interferon solution with an immobilized carrier which is coupled with a blue dye and a chelating carrier which contains a chelating residue chelated with at least one metal ion selected from the group consisting of Co 2+ , Ni 2+ and Zn 2+ .
Inventors
- 👤 Kazuo Hosoi
- 👤 Hitoshi Ozawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 23:47
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/565; Y; Y10; Y10S; Y10S435/00; Y10S435/811
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
104 words
A method of purifying human beta-interferon, comprising the successive steps of: (a) adsorbing a crude beta-human interferon solution on an immobilized blue carrier, (b) contacting the immobilized blue carrier with an eluant to produce an eluate containing the interferon, (c) contacting said eluate with a carrier having a chelating residue and including at least one metal ion selected from the group consisting of Co 2+ , Ni 2+ and Zn 2+ , wherein said metal ion is chelated to the chelating residue so a to absorb the interferon, and (d) contacting said carrier with an eluant to produce a purified and concentrated interferon solution.
Claim 2
Independent Claim
104 words
A method of purifying human beta-interferon, comprising the successive steps of: (a) adsorbing a crude beta-human interferon solution on an immobilized blue carrier, (b) contacting the immobilized blue carrier with an eluant to produce an eluate containing the interferon, (c) contacting said eluate with a carrier having a chelating residue and including at least one metal ion selected from the group consisting of Co 2+ , Ni 2+ and Zn 2+ , wherein said metal ion is chelated to the chelating residue so a to absorb the interferon, and (d) contacting said carrier with an eluant to produce a purified and concentrated interferon solution.
Claim 3
Dependent Claim
19 words
The method according to claim 1, wherein said immobilized blue carrier is an immobilized carrier coupled with a dye.
Claim 4
Dependent Claim
14 words
The method according to claim 2, wherein said dye is CI reactive blue 2.
Claim 5
Dependent Claim
14 words
The method according to claim 2, wherein said immobilized carrier is cross-linked agarose gel.
Claim 6
Dependent Claim
32 words
The method according to claim 1, wherein said immobilized blue carrier is a carrier including a coupling of the CI reactive blue 2 to the cross-linked agarose gel via an ether linkage.
Claim 7
Dependent Claim
50 words
The method according to claim 1, wherein said carrier is a water-insoluble polysaccharide derivative having a chelating residue and including at least one metal ion selected from the group consisting of Co 2+ , Ni 2+ and Zn 2+ , wherein said metal ion is chelated to the chelating residue.
Claim 8
Dependent Claim
50 words
The method according to claim 1, wherein said carrier is a cross-linked polyolefine derivative having a chelating residue and including at least one metal ion selected from the group consisting of Co 2+ , Ni 2+ and Zn 2+ , wherein said metal ion is chelated to the chelating residue.
Claim 9
Dependent Claim
54 words
The method according to claim 1, wherein said carrier is a vinyl polymer containing a hydroxy group which has a chelating residue and includes at least one metal ion selected from the group consisting of Co 2+ , Ni 2+ and Zn 2+ , wherein said metal ion is chelated to the chelating residue.
Claim 10
Dependent Claim
20 words
The method according to claim 8, wherein said vinyl polymer containing a hydroxy group is hydrophilic and globular in form.
Claim 11
Independent Claim
18 words
The method according to anyone of claims 6 to 8, wherein said chelating residue is a biscarboxymethylimino group.
Claim 12
Dependent Claim
14 words
The method according to claim 1, wherein said metal ion is Co 2+ .
Claim 13
Dependent Claim
14 words
The method according to claim 1, wherein said metal ion is Ni 2+ .
Claim 14
Dependent Claim
14 words
The method according to claim 1, wherein said metal ion is Zn 2+ .
Claim 15
Dependent Claim
17 words
The method according to claim 1, wherein said eluant contacted with the immobilized blue dextran is ethyleneglycol.
Claim 16
Dependent Claim
17 words
The method according to claim 1, wherein said eluant contacted with the carrier is an acid solution.
Claim 17
Dependent Claim
19 words
The method according to claim 1, wherein said eluant contacted with the carrier is a solution of chelating reagent.
Claim 18
Dependent Claim
16 words
The method according to claim 16, wherein said solution of chelating agent is a histidine solution.
Claim 19
Dependent Claim
16 words
The method according to claim 16, wherein said solution of chelating reagent is an EDTA solution.
Claim 20
Dependent Claim
16 words
The method according to claim 1, wherein said human β-interferon is produced by a human fibroblast.
Claim 21
Dependent Claim
21 words
The method according to claim 1, wherein said human interferon is produced by a microorganism to which interferon genes are integrated.
Claim 22
Dependent Claim
75 words
The method according to claim 1, wherein said immobilized blue carrier is a carrier including a coupling of the CI reactive blue 2 to the cross-linked agarose gel via an ether linkage and said carrier is water-insoluble polysaccharide derivative having a chelating residue and including at least one metal ion selected from the group consisting of Co 2+ , Ni 2+ and Zn 2+ , wherein said metal ion is chelated to the chelating residue.
Claim 23
Dependent Claim
14 words
The method according to claim 21, wherein said metal ion is Zn 2+ .
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-09-16 | 1989-03-18 | 1989-09-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-16 | 1993-03-18 | 1993-09-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-09-16 | 1997-03-18 | 1997-09-17 | $7700.00 | Window Closed / Lapsed |