US4257938A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purification method of human fibroblast interferon
Assignee: Toray Industries Inc
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Filing: 1979-10-29
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Granted: 1981-03-24
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Expires: 1999-10-29
Abstract
Abstract A method for the purification of human fibroblast interferon by the successive treatments of a crude interferon solution with a strongly acidic ion exchanger and a chelating carrier which contains a chelating residue chelated with at least one metal ion selected from the group consisting of Co ++ , Ni ++ , Zn ++ , and Cu ++ .
Inventors
- 👤 Kazuo Hosoi
- 👤 Hitoshi Ozawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:02
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/565; Y; Y10; Y10S; Y10S210/00; Y10S210/902; Y10S210/908
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
128 words
In a method of purifying human fibroblast interferon, the steps which comprise successively (a) absorbing an acidified solution of crude human fibroblast interferon upon a strongly acidic ion exchanger, (b) contacting the ion exchanger with an eluant which is substantially neutral or weakly basic for a time sufficient to produce an effluent containing the interferon, (c) contacting said effluent with a carrier having a chelating residue and including at least one metal ion selected from the group consisting of Co ++ , Ni ++ , Zn ++ , and Cu ++ , wherein said metal ion is chelated to the chelating residue so as to absorb the interferon, and (d) contacting said carrier with an eluant for a time sufficient to produce a purified and concentrated interferon solution.
Claim 2
Dependent Claim
128 words
In a method of purifying human fibroblast interferon, the steps which comprise successively (a) absorbing an acidified solution of crude human fibroblast interferon upon a strongly acidic ion exchanger, (b) contacting the ion exchanger with an eluant which is substantially neutral or weakly basic for a time sufficient to produce an effluent containing the interferon, (c) contacting said effluent with a carrier having a chelating residue and including at least one metal ion selected from the group consisting of Co ++ , Ni ++ , Zn ++ , and Cu ++ , wherein said metal ion is chelated to the chelating residue so as to absorb the interferon, and (d) contacting said carrier with an eluant for a time sufficient to produce a purified and concentrated interferon solution.
Claim 3
Dependent Claim
22 words
The method according to claim 1, wherein said strongly acidic ion exchanger is a water insoluble polysaccharide derivative containing a sulfonyl group.
Claim 4
Dependent Claim
16 words
The method according to claim 2, wherein said water insoluble polysaccharide derivative is a cross-linked dextran.
Claim 5
Dependent Claim
18 words
The method according to claim 1, wherein said carrier is a water-insoluble polysaccharide derivative having a chelating residue.
Claim 6
Dependent Claim
17 words
The method according to claim 1, wherein said carrier is an agarose derivative containing a biscarboxymethylamino group.
Claim 7
Dependent Claim
35 words
The method according to claim 1, wherein said strongly acidic ion exchanger is a water insoluble polysaccharide derivative containing a sulfonyl group, and said carrier is a water insoluble polysaccharide derivative containing a chelating residue.
Claim 8
Dependent Claim
31 words
The method according to claim 1, wherein said strongly acidic ion exchange is a cross-linked dextran containing a sulfonyl group, and said carrier is an agarose compound containing a biscarboxymethylamino group.
Claim 9
Dependent Claim
14 words
The method according to claim 1, wherein said metal ion is Co ++ .
Claim 10
Dependent Claim
14 words
The method according to claim 1, wherein said metal ion is Ni ++ .
Claim 11
Dependent Claim
14 words
The method according to claim 1, wherein said metal ion is Cu ++ .
Claim 12
Dependent Claim
14 words
The method according to claim 1, wherein said metal ion is Zn ++ .
Claim 13
Dependent Claim
17 words
The method according to claim 1, wherein said eluant contacted with the carrier is a histidine solution.
Claim 14
Dependent Claim
20 words
The method according to claim 1, wherein said eluant contacted with the carrier is an acidic solution of pH 1-6.
Claim 15
Dependent Claim
34 words
The method according to claim 1, wherein said chelating residue is a biscarboxymethylamino group, said metal ion is Zn ++ and said eluant contacted by the carrier is an acidic solution of pH 1-6.
References Cited (Backward Citations)
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Cited
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Cited
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-23 | 1984-09-22 | 1985-03-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-23 | 1988-09-22 | 1989-03-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-23 | 1992-09-22 | 1993-03-24 | $7700.00 | Window Closed / Lapsed |