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US4536479A
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Use of anti-idiotype antibodies in immunoassays
Assignee: NEW ENGLAND NUCLEAR Corp A CORP OF
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Filing: 1983-03-22
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Granted: 1985-08-20
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Expires: 2003-03-22
Abstract
Abstract The use of anti-idiotype antibodies as functional substitutes for antigens or haptens in immunoassays is disclosed.
Inventors
- 👤 Ronald Vander-Mallie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 11:26
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6854; G01N33/686; G01N33/53; G01N33/543; G01N33/54313; Y; Y10; Y10S; Y10S435/00; Y10S435/965; Y10S435/966; Y10S436/00; Y10S436/80; Y10S436/819
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
135 words
A homogeneous immunoassay for the detection of a nonimmunoglobulin analyte in a test sample, comprising: (1) contacting the test sample with reagents comprising: (a) a first covalent conjugate comprising a first enzyme and an idiotypic anti-analyte antibody; (b) a second covalent conjugate comprising a second enzyme and an anti-idiotypic antibody capable of competing with the analyte for the idiotypic anti-analyte antibody; (c) a substrate for the first and or second enzyme wherein one of the enzymes and the substrate are capable of reacting to produce a first product, which product is a substrate for the other enzyme, which is capable of reacting with the first product to produce a second, detectable product; and (2) measuring the amount of second product which is inversely related to the amount of analyte initially present in the test sample.
Claim 2
Dependent Claim
135 words
A homogeneous immunoassay for the detection of a nonimmunoglobulin analyte in a test sample, comprising: (1) contacting the test sample with reagents comprising: (a) a first covalent conjugate comprising a first enzyme and an idiotypic anti-analyte antibody; (b) a second covalent conjugate comprising a second enzyme and an anti-idiotypic antibody capable of competing with the analyte for the idiotypic anti-analyte antibody; (c) a substrate for the first and or second enzyme wherein one of the enzymes and the substrate are capable of reacting to produce a first product, which product is a substrate for the other enzyme, which is capable of reacting with the first product to produce a second, detectable product; and (2) measuring the amount of second product which is inversely related to the amount of analyte initially present in the test sample.
Claim 3
Dependent Claim
22 words
The immunoassay of claim 1 wherein one enzyme is glucose oxidase, the substrate is glucose and the other enzyme is horseradish peroxidase.
Claim 4
Dependent Claim
35 words
A homogeneous immunoassay for the detection of a nonimmunoglobulin analyte in a test sample comprising: (1) forming a reaction mixture by contacting the test sample with reagents, comprising: (a) a first covalent conjugate comprising a
Claim 5
Dependent Claim
35 words
A homogeneous immunoassay for the detection of a nonimmunoglobulin analyte in a test sample comprising: (1) forming a reaction mixture by contacting the test sample with reagents, comprising: (a) a first covalent conjugate comprising a
Claim 6
Dependent Claim
117 words
first fluorophore and an idiotypic anti-analyte antibody; and (b) a second covalent conjugate comprising a second fluorophore and an anti-idiotypic antibody capable of competing with the analyte for the idiotypic anti-analyte antibody; wherein one of the fluorophores is capable of absorbing incident light at a wavelength λ inc to produce light emission at wavelength λ em .sbsb.1 which can be absorbed by the other fluorophore to produce emission at wavelength λ em .sbsb.2 ; (2) irradiating the reaction mixture with light of wavelength λ inc ; and (3) measuring the intensity of light of wavelength λ em .sbsb.1 or λ em .sbsb.2, which intensity is related to the amount of analyte initially present in the test sample.
Claim 7
Dependent Claim
117 words
first fluorophore and an idiotypic anti-analyte antibody; and (b) a second covalent conjugate comprising a second fluorophore and an anti-idiotypic antibody capable of competing with the analyte for the idiotypic anti-analyte antibody; wherein one of the fluorophores is capable of absorbing incident light at a wavelength λ inc to produce light emission at wavelength λ em .sbsb.1 which can be absorbed by the other fluorophore to produce emission at wavelength λ em .sbsb.2 ; (2) irradiating the reaction mixture with light of wavelength λ inc ; and (3) measuring the intensity of light of wavelength λ em .sbsb.1 or λ em .sbsb.2, which intensity is related to the amount of analyte initially present in the test sample.
Claim 8
Dependent Claim
16 words
The immunoassay of claim 3 wherein one fluorophore is rhodamine and the other fluorophore is fluorescein.
References Cited (Backward Citations)
US4048298A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-08-19 | 1989-02-18 | 1989-08-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-08-19 | 1993-02-18 | 1993-08-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-08-19 | 1997-02-18 | 1997-08-20 | $7700.00 | Window Closed / Lapsed |