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US4535060A
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Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
Assignee: H & Q VENTURES III A CA LP
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Filing: 1983-01-05
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Granted: 1985-08-13
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Expires: 2003-01-05
Abstract
Abstract Enhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host. A mutated aroA gene is employed which expresses 5-enolpyruvyl-3-phosphoshikimate synthetase (EC: 2.5.1.19) (ES-3-P synthetase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the structural gene into a sensitive host, as well as providing a method of producing the enzyme. The E. coli strain C600(pPMG1) has been deposited at the A.T.C.C. on Dec. 14, 1982 and been given A.T.C.C. accession no. 39256.
Inventors
- 👤 Luca Comai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 22:09
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/1085; C12N9/1092; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8274; C12N15/8275; C12Q; C12Q1/00; C12Q1/48; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/581
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
58 words
A culture of cells consisting essentially of cells containing glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase having at least one mutation originating from in vitro mutation in the aro locus for the structural gene expressing said synthetase, wherein glyphosate inhibition of said synthetase containing said mutation is less than about one-half of the synthetase of the original strain prior to mutation.
Claim 2
Dependent Claim
58 words
A culture of cells consisting essentially of cells containing glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase having at least one mutation originating from in vitro mutation in the aro locus for the structural gene expressing said synthetase, wherein glyphosate inhibition of said synthetase containing said mutation is less than about one-half of the synthetase of the original strain prior to mutation.
Claim 3
Dependent Claim
17 words
A culture of cells according to claim 1, wherein said structural gene is on an episomal element.
Claim 4
Dependent Claim
13 words
A culture of cells according to claim 2, wherein said cells are prokaryotic.
Claim 5
Dependent Claim
19 words
A DNA sequence of less than 5 Kb having a structural gene coding for a glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase.
Claim 6
Dependent Claim
19 words
A DNA sequence of less than 5 Kb having a structural gene coding for a glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase.
Claim 7
Dependent Claim
39 words
A DNA sequence according to claim 4, having a mutation originating from in vitro mutation, wherein glyphosate inhibition of said synthetase containing said mutation is less than about one-half of the synthetase of the original strain prior to mutation.
Claim 8
Dependent Claim
14 words
A DNA sequence according to claim 5, wherein said synthetase is mutated prokaryotic synthetase.
Claim 9
Dependent Claim
22 words
A DNA construct comprising a DNA sequence according to claim 4 and a replication system capable of replication in a cellular host.
Claim 10
Dependent Claim
17 words
A DNA construct according to claim 7, wherein said replication system is recognized by a prokaryotic host.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-08-12 | 1989-02-11 | 1989-08-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-08-12 | 1993-02-11 | 1993-08-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-08-12 | 1997-02-11 | 1997-08-13 | $7700.00 | Window Closed / Lapsed |