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US4535059A
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Muconic acid productivity by a stabilized mutant microorganism population
Assignee: Celgene Corp
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Filing: 1983-01-13
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Granted: 1985-08-13
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Expires: 2003-01-13
Abstract
Abstract This invention provides an improved fermentation process for bioconversion of toluene to muconic acid. The process involves operating the bioconversion system under phosphate-limiting conditions so as to achieve an increase in specific muconic acid productivity with a stabilized population of microorganism such as an ATCC No. 31,916 type of Pseudomonas putida Biotype A mutant strain.
Inventors
- 👤 Jih-Han Hsieh
- 👤 Sol J. Barer
- 👤 Peter C. Maxwell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:49
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/44; Y; Y10; Y10S; Y10S435/00; Y10S435/813; Y10S435/8215; Y10S435/822; Y10S435/874; Y10S435/877
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
125 words
In a fermentation process for the production of muconic acid involving the culturing of a mutant Pseudomonad having the identifying characteristics of Pseudomonas putida ATCC No. 31,916 under conditions permitting the formation of muconic acid and recovery of muconic acid wherein the improvement comprises: limiting the presence of essential phosphate nutrient within a phosphate: growth carbon weight ratio range between about 0.04-0.08:1 in the aqueous fermentation medium which is maintained at a pH between about 5 to 8; thereby (1) increasing the specific muconic acid productivity of the microorganism, and (2) stabilizing the microorganism population whereby cell reversion is suppressed, wherein said suppressed cell reversion is to a parent strain of the microorganism which has the ability to grow on a non-growth toluene carbon source.
Claim 2
Dependent Claim
125 words
In a fermentation process for the production of muconic acid involving the culturing of a mutant Pseudomonad having the identifying characteristics of Pseudomonas putida ATCC No. 31,916 under conditions permitting the formation of muconic acid and recovery of muconic acid wherein the improvement comprises: limiting the presence of essential phosphate nutrient within a phosphate: growth carbon weight ratio range between about 0.04-0.08:1 in the aqueous fermentation medium which is maintained at a pH between about 5 to 8; thereby (1) increasing the specific muconic acid productivity of the microorganism, and (2) stabilizing the microorganism population whereby cell reversion is suppressed, wherein said suppressed cell reversion is to a parent strain of the microorganism which has the ability to grow on a non-growth toluene carbon source.
Claim 3
Dependent Claim
27 words
A fermentation process in accordance with claim 1 wherein the specific productivity rate is 0.3-0.6 grams of muconic acid per gram of dry cell weight per hour.
Claim 4
Dependent Claim
28 words
A fermentation process in accordance with claim 1 wherein the muconic acid accumulates in the aqueous fermentation medium to a concentration level above about 12 grams per liter.
Claim 5
Dependent Claim
28 words
A fermentation process in accordance with claim 1 wherein the aqueous fermentation medium contains cell growth essential nutrients comprising soluble nitrogen, calcium, magnesium, iron, potassium, and sodium compounds.
Claim 6
Dependent Claim
21 words
A fermentation process in accordance with claim 1 wherein the microorganism is Pseudomonas putida Biotype A mutant strain ATCC No. 31,916.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-08-12 | 1989-02-11 | 1989-08-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-08-12 | 1993-02-11 | 1993-08-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-08-12 | 1997-02-11 | 1997-08-13 | $7700.00 | Window Closed / Lapsed |