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US4533635A
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Process for stimulating the growth of epidermal cells
Assignee: Institut National de la Santé et de la Recherche Médicale
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Filing: 1980-07-10
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Granted: 1985-08-06
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Expires: 2000-07-10
Abstract
Abstract A process for stimulating the growth of human epidermal cells, applications of said process and products applying the said process. A process for stimulating the growth of epidermis cells. The epidermis cells are contacted with an aqueous saline extract (RE) of ocular tissues. The process is especially applied for human epidermis cells. Application to the study of chromosomes of the human epidermis cell, particularly for the detection of natural or induced chromosome anomalies, and to the production of grafts, and especially autografts, usable on man, for example in the treatment of burns, cosmetic and diagnostic products.
Inventors
- 👤 Isabelle Guedon born Saglier
- 👤 Denis Barritault
- 👤 Yves Courtois
- 👤 Cristina Arruti
- 👤 Michel Prunieras
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 15:20
CPC Classifications
A; A61; A61Q; A61Q19/00; A61K; A61K35/00; A61K35/12; A61K35/36; A61K35/44; A61K8/00; A61K8/18; A61K8/96; A61K8/98; A61K8/981; A61L; A61L27/00; A61L27/50; A61L27/60; A61Q5/00; C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0625; C12N5/0629; G; G01; G01N; G01N33/00; G01N33/48; A61Q19/08; C12N2500/00; C12N2500/70; C12N2500/80; C12N2500/84
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
24 words
A process for stimulating the growth of epiderm cells, which comprises contacting the epiderm cells with an aqueous saline extract of ocular tissue (RE).
Claim 2
Dependent Claim
24 words
A process for stimulating the growth of epiderm cells, which comprises contacting the epiderm cells with an aqueous saline extract of ocular tissue (RE).
Claim 3
Dependent Claim
25 words
The process according to claim 1, wherein the active agent is extracted from eye tissues inclusive of the choroid, the iris and the vitreous humour.
Claim 4
Dependent Claim
30 words
The process according to claim 2, wherein the RE is obtained by the treatment of bovine ocular tissues with a buffered aqueous saline solution with a pH of about 7.2.
Claim 5
Dependent Claim
25 words
The process according to claim 3, wherein there is used 50 to 400 μg, in total protein, of extract per ml of epidermal cell culture.
Claim 6
Dependent Claim
16 words
The process according to claim 4, wherein the culture is a culture of human epiderm cells.
Claim 7
Dependent Claim
22 words
The process according to claim 5 wherein said human epiderm cell is suitable for the detection of natural or induced chromosome anomalies.
Claim 8
Dependent Claim
29 words
The process according to claim 6 wherein said human epiderm cell is suitable for the assessment of sister chromatid exchanges (SCE) in in vitro cultures of adult human keratinocytes.
Claim 9
Dependent Claim
66 words
The process according to claim 7, which further comprises first effecting a preliminary normal culturing of epidermal cells in the absence of the growth factor RE, and then introducing therein after a period of about 5 to 10 days, an effective amount of RE and, then introducing about one or two days later, another effective amount of RE and obtaining a second replication of the cells.
Claim 10
Dependent Claim
33 words
The process according to claim 7, wherein the culturing of epidermal cells is effected by introducing, at the beginning, an effective amount of RE and then arresting the culturing to permit cell synchronization.
Claim 11
Dependent Claim
9 words
The process according to claim 5 resulting in grafts.
Claim 12
Dependent Claim
80 words
The process according to claim 10 which further comprises integrating said process into a grafting technique comprising removing small fragments of skin for a subsequent autograft, splitting the graft by enzymatic means 1 to 2 weeks before the culture, causing the cultured epidermal cells to proliferate on a substrate comprising thin flaps of human skin or pig skin, the RE being added in an effective amount for quick cultivation of epidermal cells, and effecting the graft in a conventional manner.
Claim 13
Dependent Claim
16 words
The process of anyone of claims 1 to 5 comprising the long-term culturing of epidermal cells.
Claim 14
Dependent Claim
31 words
The process according to claim 12, wherein the cells are cultured directly on a substrate in the presence of an effective amount of RE and without the addition of other cells.
Claim 15
Dependent Claim
14 words
The process of claim 10 wherein said graft is suitable for treatment of burns.
References Cited (Backward Citations)
US4299819A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-08-05 | 1989-02-04 | 1989-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-08-05 | 1993-02-04 | 1993-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-08-05 | 1997-02-04 | 1997-08-06 | $7700.00 | Window Closed / Lapsed |