US4533447A
Expired / Lapsed
US
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Apparatus for and method of isoelectric focussing
Assignee: Individual
•
Filing: 1983-06-13
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Granted: 1985-08-06
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Expires: 2003-06-13
Abstract
Abstract An apparatus for and a method for separating a mixture of proteins containing proteins of different isoelectric points, which method comprises flowing an aqueous feed stream containing the mixture of proteins along a thin-channel flow path established between first and second gas-permeable membranes, diffusing a gas through the first and second membranes and generally transversely across the flow path of the feed stream, the gas selected to establish a gradient in the concentration of the gas and to provide a gradient in pH across the flow path of the feed stream, and, after a defined time period, separating the feed stream into streams containing concentrated fractions of proteins having defined isoelectric points, by virtue of the difference in the direction of diffusion occurring of the proteins in the flow path.
Inventors
- 👤 Jerry H. Meldon
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 22:46
CPC Classifications
B; B01; B01D; B01D57/00; B01D57/02; Y; Y10; Y10S; Y10S210/00; Y10S210/902; Y10S210/903; Y10S210/905
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
125 words
A method of separating a feed stream composed of a mixture of materials having different isoelectric points, into one or more concentrated streams containing materials of defined isoelectric points, which method comprises: (a) establishing a concentration gradient of a gas stream across a path of flow of the feed streams, the gas stream selected to provide the feed stream with a pH gradient across the flow path of the feed stream; and (b) separating the materials having different isoelectric points into concentrated effluent streams of defined isoelectric points, such separation occurring by virtue of the dependence of the directions of diffusion of the different feed materials upon the isoelectric points of the feed materials which occurs in the gradient established across the path of flow.
Claim 2
Independent Claim
125 words
A method of separating a feed stream composed of a mixture of materials having different isoelectric points, into one or more concentrated streams containing materials of defined isoelectric points, which method comprises: (a) establishing a concentration gradient of a gas stream across a path of flow of the feed streams, the gas stream selected to provide the feed stream with a pH gradient across the flow path of the feed stream; and (b) separating the materials having different isoelectric points into concentrated effluent streams of defined isoelectric points, such separation occurring by virtue of the dependence of the directions of diffusion of the different feed materials upon the isoelectric points of the feed materials which occurs in the gradient established across the path of flow.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein the feed stream comprises an aqueous stream containing proteinaceous materials of different isoelectric points.
Claim 4
Dependent Claim
16 words
The method of claim 2 wherein the feed stream comprises a mixture of hemoglobin an albumin.
Claim 5
Dependent Claim
26 words
The method of claim 2, wherein said proteinaceous material is selected from the group consisting of enzymes, hormones, antibodies, amino acids, nucleic acids, albumin and hemoglobin.
Claim 6
Dependent Claim
21 words
A method according to claim 1, wherein the pH values of the materials in the feed stream differ by about 2.
Claim 7
Dependent Claim
15 words
The method of claim 1 wherein the gas stream employed is a carbon-dioxide-containing gas stream.
Claim 8
Dependent Claim
28 words
The method of claim 1 wherein the gas establishes a difference in electrical potential across the flow path of the feed stream of about 0.1 to 1.0 volt.
Claim 9
Dependent Claim
46 words
A method according to claim 1, which includes providing electrodes across the flow path of the feed stream, to supplement the naturally evolving electrical potential and so accelerate further the rate of diffusion and separation of the feed stream into one or more concentrated isoelectric fractions.
Claim 10
Dependent Claim
58 words
The method of claim 1 which includes establishing a gradient by flowing a feed stream in a flow path between gas-permeable type materials, and passing gas through the gas-permeable type materials generally transversely to the path of the flow of the feed stream between the gas-permeable-type materials, to establish the gradient in the concentration of the gas stream.
Claim 11
Dependent Claim
12 words
The method of claim 9, wherein said gas-permeable-type materials are gas-permeable membranes.
Claim 12
Dependent Claim
18 words
The method of claim 10 which includes immobilizing a catalyst on an inner surface of the gas-permeable membrane.
Claim 13
Dependent Claim
13 words
The method of claim 11, wherein the catalyst is the enzyme carbonic anhydrase.
Claim 14
Dependent Claim
15 words
A method according to claim 9, wherein the gas-permeable type materials are gas-permeable membrane-type materials.
Claim 15
Dependent Claim
141 words
The method of claim 1 which includes (a) flowing the feed stream through a narrow channel flow path established by a pair of spaced-apart, first and second gas-permeable membranes; (b) passing a carbon-dioxide-containing gas stream adjacent to an outer surface of a first membrane and an inert sweep gas on an outer surface of a second membrane, to provide for a gradient in the concentration of the carbon dioxide-containing gas stream across the first and second membranes and the narrow channel flow path of the feed stream; (c) withdrawing a carbon-dioxide-containing gas from the gas channel adjacent to the first membrane; (d) withdrawing an inert gas containing carbon dioxide from the gas channel adjacent to the second membrane; and (e) separating the feed stream across the narrow channel flow path, to recover concentrated streams containing desired materials of particular isoelectric points.
Claim 16
Dependent Claim
26 words
The method of claim 14 which includes physically dividing the feed stream into two or more concentrated feed streams, after an effective permanent degree of separation.
Claim 17
Dependent Claim
38 words
The method of claim 1 which includes providing a plurality of paths of flow of the feed stream, establishing a gradient across each path of flow and separating the material of different isoelectric points in each flow path.
Claim 18
Dependent Claim
17 words
The method of claim 1, wherein said gas stream is an acid or an alkaline gas stream.
Claim 19
Dependent Claim
26 words
The method of claim 1, wherein said gas stream comprises a gas selected from the group consisting of sulfur dioxide, carbon dioxide, hydrogen sulfide and ammonia.
Claim 20
Dependent Claim
12 words
A method according to claim 1, wherein said materials are biological materials.
Claim 21
Independent Claim
222 words
A method of separating a feed stream comprising a mixture of proteins of different isoelectric points into one or more concentrated feed streams composed of proteins of defined isoelectric fractions, which method comprises: (a) flowing an aqueous feed stream along a narrow channel flow path established between a first carbon dioxide-permeable membrane and a second carbon-dioxide-permeable membrane, each of said membranes having an inner surface facing said flow path and an outer surface facing a gas; (b) flowing a carbon-dioxide-containing gas stream adjacent the outer surface of the first membrane; (c) flowing an inert sweep gas adjacent the outer surface of the second membrane; (d) diffusing and passing the carbon-dioxide-containing gas through the first carbon-dioxide-permeable membrane in a direction generally transversely to the narrow channel flow path of the feed stream and through the second carbon-dioxide-permeable membrane, to establish a concentration gradient in the dissolved carbon-dioxide gas stream across the narrow channel flow path of the feed stream, and to provide a pH gradient across the narrow channel flow path; (e) withdrawing a mixture of the carbon-dioxide-containing-gas stream and the inert sweep gas stream from a location adjacent the second membrane; and (f) recovering, after a period of time, concentrated feed streams composed of proteins of defined isoelectric fractions, by physically separating portions of the feed stream after the period of time.
Claim 22
Independent Claim
222 words
A method of separating a feed stream comprising a mixture of proteins of different isoelectric points into one or more concentrated feed streams composed of proteins of defined isoelectric fractions, which method comprises: (a) flowing an aqueous feed stream along a narrow channel flow path established between a first carbon dioxide-permeable membrane and a second carbon-dioxide-permeable membrane, each of said membranes having an inner surface facing said flow path and an outer surface facing a gas; (b) flowing a carbon-dioxide-containing gas stream adjacent the outer surface of the first membrane; (c) flowing an inert sweep gas adjacent the outer surface of the second membrane; (d) diffusing and passing the carbon-dioxide-containing gas through the first carbon-dioxide-permeable membrane in a direction generally transversely to the narrow channel flow path of the feed stream and through the second carbon-dioxide-permeable membrane, to establish a concentration gradient in the dissolved carbon-dioxide gas stream across the narrow channel flow path of the feed stream, and to provide a pH gradient across the narrow channel flow path; (e) withdrawing a mixture of the carbon-dioxide-containing-gas stream and the inert sweep gas stream from a location adjacent the second membrane; and (f) recovering, after a period of time, concentrated feed streams composed of proteins of defined isoelectric fractions, by physically separating portions of the feed stream after the period of time.
Claim 23
Independent Claim
244 words
An apparatus for the separation of a feed stream comprising a mixture of protein materials, said materials having different isoelectric points, into one or more concentrated streams composed of proteins of defined isoelectric points, which apparatus comprises: (a) first and second gas-permeable membranes, the membranes each having an inner surface facing said feed stream and an outer surface facing a gas, the first and second membranes spaced apart to define a flow path for the feed stream between the inner surface of the first and second membranes; (b) an inlet for the introduction of a feed stream into the flow path; (c) a first gas channel disposed adjacent the outer surface of the first membrane; (d) a second gas channel disposed adjacent the outer surface of the second membrane; (e) an inlet for the introduction of a gas stream to the second gas channel; (f) an inlet for the introduction of an inert sweep gas to the first gas channel; (g) an outlet for the removal of the gas stream from the second gas channel; (h) an outlet of the removal of a mixture of the inert sweep gas and gas stream from the first gas channel; (i) an outlet in the flow path for the withdrawal of the concentrated streams composed of proteins of defined isoelectric points; and (j) means to physically divide the feeds stream at the outlet in the flow path into concentrated streams composed of proteins of defined isoelectric points.
Claim 24
Independent Claim
244 words
An apparatus for the separation of a feed stream comprising a mixture of protein materials, said materials having different isoelectric points, into one or more concentrated streams composed of proteins of defined isoelectric points, which apparatus comprises: (a) first and second gas-permeable membranes, the membranes each having an inner surface facing said feed stream and an outer surface facing a gas, the first and second membranes spaced apart to define a flow path for the feed stream between the inner surface of the first and second membranes; (b) an inlet for the introduction of a feed stream into the flow path; (c) a first gas channel disposed adjacent the outer surface of the first membrane; (d) a second gas channel disposed adjacent the outer surface of the second membrane; (e) an inlet for the introduction of a gas stream to the second gas channel; (f) an inlet for the introduction of an inert sweep gas to the first gas channel; (g) an outlet for the removal of the gas stream from the second gas channel; (h) an outlet of the removal of a mixture of the inert sweep gas and gas stream from the first gas channel; (i) an outlet in the flow path for the withdrawal of the concentrated streams composed of proteins of defined isoelectric points; and (j) means to physically divide the feeds stream at the outlet in the flow path into concentrated streams composed of proteins of defined isoelectric points.
Claim 25
Dependent Claim
26 words
The apparatus of the claim 21, wherein the means to physically divide the feed stream comprises a physical projection adjacent the outlet in the flfow path.
Claim 26
Dependent Claim
31 words
The apparatus of claim 21, wherein the first and second gas-permeable membranes are spaced apart, to define a narrow channel flow path having a distance of about 5 millimeters or less.
Claim 27
Dependent Claim
30 words
The apparatus of claim 21, which further comprises first and second electrodes spaced apart across the flow path, and means to establish an electrical potential gradient across the flow path.
Claim 28
Dependent Claim
14 words
The apparatus of claim 21, wherein the gas-permeable membrane comprises a carbon-dioxide, gas-permeable membrane.
Claim 29
Dependent Claim
24 words
The apparatus of claim 21, wherein said membranes comprise materials selected from the group consisting of cellulose acetate, polyolefin-type resin, silicone and cellulose triacetate.
Claim 30
Dependent Claim
30 words
The apparatus of claim 21, wherein the ratio of the distance between the membranes to the width of the flow path to the length of the flow path is 2:50:500.
Claim 31
Dependent Claim
24 words
The apparatus of claim 21, which includes a plurality of paths of flow defined by a plurality of sets of first and second membranes.
Claim 32
Dependent Claim
13 words
The apparatus of claim 21, wherein said membranes are stretched over porous supports.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-08-05 | 1989-02-04 | 1989-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-08-05 | 1993-02-04 | 1993-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-08-05 | 1997-02-04 | 1997-08-06 | $7700.00 | Window Closed / Lapsed |