Patent US4533307A successfully refreshed from Google Patents.
US4533307A
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Apparatus for preparing thin gel slabs for electrophoresis
Assignee: European Molecular Biology Laboratory
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Filing: 1984-01-27
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Granted: 1985-08-06
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Expires: 2004-01-27
Abstract
Abstract An apparatus for preparing thin layers, such as thin gel layers useful in electrophoresis, wherein a liquid layer material is introduced to the space defined between two parallel mold surfaces on first and second mold elements. Initially, the mold surfaces overlap each other to a small extent, and liquid is introduced to the space between the surfaces adjacent the leading edge of the top mold element. The mold elements are then shifted with respect to each other while maintaining their parallel relationship, and additional liquid layer-forming material is introduced to the space during the shift. In a preferred embodiment, a third mold element is situated adjacent to and spaced a small distance from the bottom mold elements, and liquid material is introduced to the space between the bottom mold elements during shifting of the top mold.
Inventors
- 👤 Wilheln Ansorge
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 05:50
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
114 words
An apparatus for preparing a thin layer of a thickness and a material useful in electrophoresis, comprising: (a) an elongate horizontal support having opposed sides; (b) a bottom mold element supported on said support; (c) opposed guide means on opposite sides of said support; (d) a top mold element disposed between said guide means parallel to and spaced a distance equal to the thickness to a layer useful in electrophoresis from said bottom mold element for longitudinal movement over said bottom mold element; and (e) a drive operatively attached to said top mold element for moving said top mold element relative to said bottom mold element in a forward direction parallel to said support.
Claim 2
Independent Claim
114 words
An apparatus for preparing a thin layer of a thickness and a material useful in electrophoresis, comprising: (a) an elongate horizontal support having opposed sides; (b) a bottom mold element supported on said support; (c) opposed guide means on opposite sides of said support; (d) a top mold element disposed between said guide means parallel to and spaced a distance equal to the thickness to a layer useful in electrophoresis from said bottom mold element for longitudinal movement over said bottom mold element; and (e) a drive operatively attached to said top mold element for moving said top mold element relative to said bottom mold element in a forward direction parallel to said support.
Claim 3
Dependent Claim
37 words
The apparatus of claim 1 wherein an auxiliary mold element is disposed in the plane of said bottom mold element and spaced longitudinally therefrom, and said bottom and auxiliary mold elements are disposed between said guide means.
Claim 4
Dependent Claim
42 words
The apparatus of claim 2 wherein said support comprises a track defining a horizontal upper surface and said guide means comprise L-shaped elements extending from the sides of said track, and said bottom and auxiliary mold elements lie on said horizontal surface.
Claim 5
Dependent Claim
12 words
The apparatus of claim 1 wherein said drive comprises a spindle drive.
Claim 6
Dependent Claim
20 words
The apparatus of claim 4 wherein said drive includes a spindle connected to said top mold element by a stirrup.
Claim 7
Dependent Claim
22 words
The apparatus of claim 5 wherein a first end of said stirrup is connected to a bridge on said top mold element.
Claim 8
Dependent Claim
21 words
The apparatus of claim 6 wherein a second end of said stirrup is secured to a spindle nut on the spindle.
Claim 9
Dependent Claim
29 words
The apparatus of claim 3 wherein said track comprises a downwardly open first U-shaped track, each leg of said U-track being received by a respective upwardly open U-shaped element.
Claim 10
Dependent Claim
34 words
The apparatus of claim 2 wherein a liquid supply tube is disposed in the space between said bottom and auxiliary mold elements, said tube having outlet perforations for introducing liquid material to said space.
Claim 11
Independent Claim
110 words
An apparatus for preparing a thin layer of a thickness and a material useful in electrophoresis, comprising: (a) an elongated horizontal support having opposed sides; (b) a bottom mold element supported on said support; (c) opposed guide means on opposite sides of said support; (d) a top mold element disposed between said guide means parallel to and spaced a distance of about 1.5 mm or less from said bottom mold element for longitudinal movement over said bottom mold element; and (e) a drive operatively attached to said top mold element for moving said top mold element relative to said bottom mold element in a forward direction parallel to said support.
Claim 12
Independent Claim
110 words
An apparatus for preparing a thin layer of a thickness and a material useful in electrophoresis, comprising: (a) an elongated horizontal support having opposed sides; (b) a bottom mold element supported on said support; (c) opposed guide means on opposite sides of said support; (d) a top mold element disposed between said guide means parallel to and spaced a distance of about 1.5 mm or less from said bottom mold element for longitudinal movement over said bottom mold element; and (e) a drive operatively attached to said top mold element for moving said top mold element relative to said bottom mold element in a forward direction parallel to said support.
Claim 13
Dependent Claim
12 words
The apparatus of claim 10 wherein said drive comprises a spindle drive.
Claim 14
Dependent Claim
20 words
The apparatus of claim 11 wherein said drive includes a spindle connected to said top mold element by a stirrup.
Claim 15
Dependent Claim
22 words
The apparatus of claim 12 wherein a first end of said stirrup is connected to a bridge on said top mold element.
Claim 16
Dependent Claim
21 words
The apparatus of claim 13 wherein a second end of said stirrup is secured to a spindle nut on the spindle.
Claim 17
Dependent Claim
22 words
The apparatus of claim 10 wherein said spacing between said top and bottom mold elements is between about 0.02 and 1.5 mm.
Claim 18
Dependent Claim
37 words
The apparatus of claim 10 wherein an auxiliary mold element is disposed in the plane of said bottom mold element and spaced longitudinally therefrom, and said bottom and auxiliary mold elements are disposed between said guide means.
Claim 19
Dependent Claim
34 words
The apparatus of claim 16 wherein a liquid supply tube is disposed in the space between said bottom and auxiliary mold elements, said tube having outlet perforations for introducing liquid material to said space.
Claim 20
Dependent Claim
42 words
The apparatus of claim 16 wherein said support comprises a track defining a horizontal upper surface and said guide means comprise L-shaped elements extending from the sides of said track, and said bottom and auxiliary mold elements lie on said horizontal surface.
Claim 21
Dependent Claim
29 words
The apparatus of claim 18 wherein said track comprises a downwardly open first U-shaped track, each leg of said U-track being received by a respective upwardly open U-shaped element.
Claim 22
Dependent Claim
22 words
The apparatus of claim 10 wherein said spacing between said top and bottom mold elements is between about 0.02 and 1.5 mm.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-08-05 | 1989-02-04 | 1989-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-08-05 | 1993-02-04 | 1993-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-08-05 | 1997-02-04 | 1997-08-06 | $7700.00 | Window Closed / Lapsed |