Patent US4532205A successfully refreshed from Google Patents.
US4532205A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the injection of very small amounts of samples into cells
Assignee: European Molecular Biology Laboratory
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Filing: 1983-01-21
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Granted: 1985-07-30
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Expires: 2003-01-21
Abstract
Abstract A method and a device are proposed for the injection of very small amounts of samples into cells (74) with the aid of a microcapillary (10). During the injection, a constant injection pressure of about 0.12 bar, provided by a pressurized cylinder (46) is applied to the microcapillary (10), which is reduced after the completion of the injection to a holding pressure of 0.02 bar until the next cell (64) is to be injected. A high-pressure (p 1 ) supplied from another pressurized cylinder (44) serves for blowing out the microcapillary tip (12). A three-way valve (42) permits simple and rapid switching between the various pressures (p 1 , p 2 and p 3 ). Reproducible amounts of sample in the range from 10 -11 ml to 10 -13 ml can be injected reproducibly, simply and rapidly.
Inventors
- 👤 Wilhelm Ansorge
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 06:25
CPC Classifications
G; G01; G01N; G01N35/00; G01N35/10; G01N35/1095; G01N35/1097; Y; Y10; Y10T; Y10T137/00; Y10T137/8158; Y10T137/8326; Y10T137/8593; Y10T137/87249; Y10T137/87265
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
127 words
A method of injecting very small samples of fluid into cells using a microcapillary having an inside space, said microcapillary having a first end provided with a tip and a second end opposite said tip, said method comprising the steps of: (A) filling said inside space with said sample fluid; (B) applying an injection pressure to said inside space from said second capillary end; (C) injecting a desired amount of sample fluid through said tip into a cell; (D) reducing the pressure applied to said inside space after injection to a holding pressure that is lower than said injection pressure and greater than zero whereby substantially no sample fluid flows out from the tip; and (E) maintaining said holding pressure until the beginning of the next injection.
Claim 2
Independent Claim
127 words
A method of injecting very small samples of fluid into cells using a microcapillary having an inside space, said microcapillary having a first end provided with a tip and a second end opposite said tip, said method comprising the steps of: (A) filling said inside space with said sample fluid; (B) applying an injection pressure to said inside space from said second capillary end; (C) injecting a desired amount of sample fluid through said tip into a cell; (D) reducing the pressure applied to said inside space after injection to a holding pressure that is lower than said injection pressure and greater than zero whereby substantially no sample fluid flows out from the tip; and (E) maintaining said holding pressure until the beginning of the next injection.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein said holding pressure is 0.1 to 0.5 times said injection pressure.
Claim 4
Dependent Claim
36 words
The method of claim 1 wherein the pressure applied to said inside space is increased to a high pressure which is higher than said injection pressure after step (A) to prevent initial blocking of said tip.
Claim 5
Dependent Claim
39 words
The method of claim 1 wherein the pressure applied to said inside space is increased to a high pressure higher than said injection pressure after several injections to prevent blocking of or formation of deposits inside said capillary tip.
Claim 6
Dependent Claim
19 words
The method of claim 3 or 4 wherein said high pressure is 5 to 100 times said injection pressure.
Claim 7
Dependent Claim
16 words
The method of claim 5 wherein said high pressure is about 17 times said injection pressure
Claim 8
Dependent Claim
15 words
The method of claim 1 wherein said injection pressure is between 0.1 to 0.5 bar.
Claim 9
Dependent Claim
13 words
The method of claim 7 wherein said injection pressure is about 0.12 bar.
Claim 10
Dependent Claim
19 words
The method of claim 1 wherein said sample fluid is filled from the capillary end opposite said microcapillary tip.
Claim 11
Dependent Claim
61 words
The method of claim 9, wherein said fluid filling is accomplished with the aid of a filling capillary, the outside diameter of which is smaller than the inside diameter of said microcapillary, said filling capillary being inserted into said capillary end of said microcapillary opposite said tip and extended within the microcapillary to a point adjacent the tip of said microcapillary.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-07-29 | 1989-01-28 | 1989-07-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-07-29 | 1993-01-28 | 1993-07-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-07-29 | 1997-01-28 | 1997-07-30 | $7700.00 | Window Closed / Lapsed |