US4525457A
Expired / Lapsed
US
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Method of immobilizing enzymatically active materials
Assignee: Eneos Corp
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Filing: 1983-04-22
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Granted: 1985-06-25
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Expires: 2003-04-22
Abstract
Abstract This invention discloses a method of immobilizing an enzymatically active material to a water-insoluble polyanion which comprises bringing a polycation-containing aqueous medium and the enzymatically active substance into contact with the water-insoluble polyanion having any desired form. This method is characterized in that the enzymatically active substance is adsorbed on the surfaces of the water-insoluble polyanion and, at the same time, a strong complex is formed by the interaction of the polycation and the polyanion to produce a water-insoluble film. As a result, the enzymatically active substance so adsorbed is securely fixed to the surfaces of the water-insoluble polyanion and scarcely released therefrom.
Inventors
- 👤 Ko Sakata
- 👤 Hirosuke Imai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 23:24
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/08; C12N11/082; C12N11/087
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
44 words
A method of immobilizing enzymatically active materials which comprises bringing an aqueous medium comprising a polycation and an enzymatically active material into contact with a water-insoluble polyanion, whereby the enzymatically active material is fixed as a film on the surface of the water-insoluble polyanion.
Claim 2
Independent Claim
44 words
A method of immobilizing enzymatically active materials which comprises bringing an aqueous medium comprising a polycation and an enzymatically active material into contact with a water-insoluble polyanion, whereby the enzymatically active material is fixed as a film on the surface of the water-insoluble polyanion.
Claim 3
Dependent Claim
62 words
The method of claim 1 wherein the polycation comprises at least one polymer selected from polymers containing a quaternary ammonium ion group in the backbone, polymers containing a quaternary ammonium salt group in the side chains, polymers containing an amine group or a salt thereof in the backbone, or polymers containing an amine group or a salt thereof in the side chains.
Claim 4
Dependent Claim
24 words
The method of claim 1 wherein the enzymatically active material comprises a member selected from the group consisting of enzymes, microorganisms and cell fractions.
Claim 5
Dependent Claim
57 words
The method of claim 1 wherein the water-insoluble polyanion comprises at least one polymer selected from polymers containing a carboxylic acid group or a salt thereof, polymers containing a sulfonic acid group or a salt thereof, polymers containing a sulfuric ester group or a salt thereof, or polymers containing a phosphoric ester group or a salt thereof.
Claim 6
Dependent Claim
45 words
The method of claim 1 wherein the water-insoluble polyanion and the polycation-containing aqueous medium are used in such a proportion that the ratio of the equivalents of the cationic group to the equivalents of the anionic group is in the range of 0.01 to 100.
Claim 7
Dependent Claim
36 words
The method of claim 1 wherein the temperature at which the polycation-containing aqueous medium and the enzymatically active material are brought into contact with the water-insoluble polyanion is in the range of -20° to 100° C.
References Cited (Backward Citations)
US4194066A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-06-24 | 1988-12-24 | 1989-06-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-06-24 | 1992-12-24 | 1993-06-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-06-24 | 1996-12-24 | 1997-06-25 | $7700.00 | Window Closed / Lapsed |