US4521519A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagent for the precipitation of apo-B-containing lipoproteins
Assignee: Roche Diagnostics GmbH
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Filing: 1982-04-19
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Granted: 1985-06-04
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Expires: 2002-04-19
Abstract
Abstract The present invention provides a reagent for the precipitation of apo-B-containing lipoproteins, wherein it comprises 0.2 to 3 grams per liter of phosphotungstic acid and more than 2 mmols per liter of magnesium ions in aqueous solution. The present invention also provides a process for the preparation of this reagent and a process for the precipitation of apo-B-containing lipoproteins using this reagent.
Inventors
- 👤 Brigitte Draeger
- 👤 Joachim Ziegenhorn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 18:54
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92; G01N2800/00; G01N2800/04; G01N2800/044; Y; Y10; Y10S; Y10S436/00; Y10S436/825; Y10T; Y10T436/00; Y10T436/10; Y10T436/107497; Y10T436/25
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
30 words
Reagent for the precipitation of apo-B-containing lipoproteins, comprising 0.2 to 3 grams of phosphotungstic acid per liter and at least 2 mmols of magnesium ions per liter in aqueous solution.
Claim 2
Dependent Claim
30 words
Reagent for the precipitation of apo-B-containing lipoproteins, comprising 0.2 to 3 grams of phosphotungstic acid per liter and at least 2 mmols of magnesium ions per liter in aqueous solution.
Claim 3
Dependent Claim
16 words
Reagent as claimed in claim 1, comprising 0.25 to 2 grams of phosphotungstic acid per liter.
Claim 4
Dependent Claim
16 words
Reagent as claimed in claim 1, comprising 4 to 25 mmols of magnesium ions per liter.
Claim 5
Dependent Claim
26 words
Reagent as claimed in claim 1, comprising 0.25 to 2 grams of phosphotungstic acid per liter and 4 to 25 mmols of magnesium ions per liter.
Claim 6
Dependent Claim
14 words
Reagent as claimed in claim 1, having a pH value from 2 to 8.
Claim 7
Dependent Claim
21 words
Reagent as claimed in claim 5, wherein the pH is adjusted to 2 to 8 by the addition of sodium hydroxide.
Claim 8
Dependent Claim
11 words
Reagent as claimed in claim 1, further comprising a non-ionic detergent.
Claim 9
Dependent Claim
11 words
Reagent as claimed in claim 1, further comprising an anionic detergent.
Claim 10
Dependent Claim
20 words
Reagent as claimed in claim 7, wherein the non-ionic detergent is a polyoxyethylenelauryl ether, polyoxyethylenealkylaryl ether, polyoxyethylated octylphenol or hydroxypolyethoxydodecane.
Claim 11
Dependent Claim
20 words
Reagent as claimed in claim 8, wherein the anionic detergent is tetrasodium N-(1,2-dicarboxyethyl)-N-octadecylsulphosuccinamate, disodium N-octadecylsulphosuccinamate, sodium dioctylsulphosuccinate or disodium dodecylsulphosuccinate.
Claim 12
Dependent Claim
19 words
Reagent as claimed in claim 7, wherein the detergent is present in a concentration of from 0.03 to 0.3%.
Claim 13
Dependent Claim
19 words
Reagent as claimed in claim 8, wherein the detergent is present in a concentration of from 0.03 to 0.3%.
Claim 14
Dependent Claim
35 words
Process for the preparation of the reagent claimed in claim 1 which process comprises dissolving phosphotungstic acid and a water soluble magnesium salt in water and adjusting to a pH value of 2 to 8.
Claim 15
Dependent Claim
25 words
Process as claimed in claim 13, wherein the pH value is adjusted to a value of 2 to 8 by the addition of a base.
Claim 16
Dependent Claim
26 words
Process as claimed in claim 13, wherein the phosphotungstic acid and the magnesium salt are separately dissolved in water and the two solutions subsequently mixed together.
Claim 17
Dependent Claim
28 words
Process as claimed in claim 13, wherein 0.25 to 2 grams per liter of phosphotungstic acid and 4 to 25 mmols per liter of magnesium ions are dissolved.
Claim 18
Dependent Claim
32 words
Process for the precipitation of apo-B-containing lipoproteins which process comprises adding to the sample to measured, the reagent of claim 1, mixing the sample and reagent and allowing the precipitate to form.
Claim 19
Dependent Claim
23 words
Process as claimed in claim 17, wherein a diluent is first added to the sample to be measured, followed by adding said reagent.
Claim 20
Dependent Claim
25 words
Process as claimed in claim 17, wherein 1 part by volume of sample is mixed with 2 to 20 parts by volume of said reagent.
Claim 21
Dependent Claim
42 words
Process as claimed in claim 17, wherein 1 part by volume of sample is mixed with 2 parts by volume of said reagent which contains 1.8 to 1.9 grams per liter of phosphotungstic acid and 25 mmols per liter of magnesium ions.
Claim 22
Dependent Claim
42 words
Process as claimed in claim 17, wherein 1 part by volume of sample is mixed with 2.5 parts by volume of said reagent which contains 1.4 to 1.5 grams per liter of phosphotungstic acid and 20 mmols per liter of magnesium ions.
Claim 23
Dependent Claim
34 words
Process as claimed in claim 17, wherein 1 part by volume of sample is mixed with 20 parts by volume of said reagent which contains 0.25 to 0.35 grams per liter of phosphotungstic acid.
Claim 24
Dependent Claim
17 words
Process as claimed in claim 18, wherein a non-ionic or anionic detergent is admixed with the diluent.
Claim 25
Dependent Claim
36 words
Process as claimed in claim 23, wherein the non-ionic detergent used in a polyoxyethylenelauryl ether, polyoxyethylenealkylaryl ether, polyoxyethylated octylphenol or hydroxypolyethoxydodecane and the anionic detergent used is tetrasodium N-(1,2-dicarboxyethyl)-N-octadecylsulphosuccinamate, disodium N-octadecylsulphosuccinamate, sodium dioctylsulphosuccinate or disodium dodecylsulphosuccinate.
Claim 26
Dependent Claim
18 words
Process as claimed in claim 23, wherein the detergent is present in a concentration of 0.1 to 1%.
Claim 27
Dependent Claim
51 words
Method for the determination of HDL cholesterol, which method comprises first precipitating out of the sample to be measured, the apo-B-containing lipoproteins, centrifuging off the resultant precipitate, determining the HDL cholesterol in the supernatant and wherein for the precipitation of the apo-B-containing lipoproteins there is added the reagent of claim 1.
Claim 28
Dependent Claim
16 words
Process as claimed in claim 17, wherein the reagent contains additionally a non-ionic or anionic detergent.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-06-03 | 1988-12-03 | 1989-06-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-06-03 | 1992-12-03 | 1993-06-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-06-03 | 1996-12-03 | 1997-06-04 | $7700.00 | Window Closed / Lapsed |