US4518698A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Plasmid and production thereof
Assignee: Takeda Pharmaceutical Co Ltd
•
Filing: 1982-06-04
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Granted: 1985-05-21
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Expires: 2002-06-04
Abstract
Abstract A novel plasmid pATM3 is obtainable from a microorganism belonging to the genus Streptomyces. The plasmid pATM3 is useful as a cloning vector in recombinant DNA work.
Inventors
- 👤 Masakazu Kikuchi
- 👤 Takaki Hayakawa
- 👤 Makoto Kida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 22:30
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/76
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
72 words
An isolated plasmid pATM3, which is characterized by the following properties: (1) Molecular weight: approximately 4.37±0.05 megadaltons, (2) Shape: circular, (3) Restriction endonuclease cleavage sites: Bam HI (0/4.4), Sal I (0.8, 0.99 or 1.00, and 1.19), Bgl II (2.9) and Xho I (4.2), (4) Sensitivity to restriction endonucleases: ______________________________________
Restriction endonuclease Number of cleavage sites
______________________________________
Bam HI 1
Bgl II 1
Sal I 3
Sma I >4
Xho I 1
______________________________________
Claim 2
Dependent Claim
72 words
An isolated plasmid pATM3, which is characterized by the following properties: (1) Molecular weight: approximately 4.37±0.05 megadaltons, (2) Shape: circular, (3) Restriction endonuclease cleavage sites: Bam HI (0/4.4), Sal I (0.8, 0.99 or 1.00, and 1.19), Bgl II (2.9) and Xho I (4.2), (4) Sensitivity to restriction endonucleases: ______________________________________
Restriction endonuclease Number of cleavage sites
______________________________________
Bam HI 1
Bgl II 1
Sal I 3
Sma I >4
Xho I 1
______________________________________
Claim 3
Independent Claim
33 words
A method for producing plasmid pATM3 which comprises cultivating Streptomyces castaneoglobisporus IFO 13669 in the culture medium, harvesting the cells therefrom,, subjecting said cells to lysis, and isolating plasmid pATM3 from the lysate.
Claim 4
Independent Claim
33 words
A method for producing plasmid pATM3 which comprises cultivating Streptomyces castaneoglobisporus IFO 13669 in the culture medium, harvesting the cells therefrom,, subjecting said cells to lysis, and isolating plasmid pATM3 from the lysate.
Claim 5
Dependent Claim
8 words
A cloning vector comprising an isolated plasmid pATM3.
Claim 6
Dependent Claim
8 words
A cloning vector comprising an isolated plasmid pATM3.
References Cited (Backward Citations)
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JPS55120600A
Cited
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Cited
JPS55133396A
Cited
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Cited
US4343906A
Cited
US4332900A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-05-20 | 1988-11-19 | 1989-05-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-05-20 | 1992-11-19 | 1993-05-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-05-20 | 1996-11-19 | 1997-05-21 | $7700.00 | Window Closed / Lapsed |