US4514508A
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Assaying for a multiplicity of antigens or antibodies with a detection compound
Assignee: Biond Inc
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Filing: 1982-07-06
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Granted: 1985-04-30
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Expires: 2002-07-06
Abstract
Abstract Immunologic assay for biological and pharmaceutical substances sets forth a universal method for the qualitative and quantitative determination of biologic and pharmaceutical substances, thus eliminating the need for multiple samples at multiple laboratories and minimizing the time factor required for such determinations. The assay is based on the recognition and binding of an antibody and an antigen or hapten to form a complex, and the changes which occur in the antibody conformation and chemical properties when such a complex is formed. A solid phase matrix, such as but not limited to a microtiter plate, is prepared having qualitative and/or quantitative spectrum of antibodies, or spectrum of antigens or haptens, bound in each position, which in the case of microtiter plates would be a sample well. The sample is then distributed to each of the positions or wells accordingly. If the sample contains an antigen or hapten that corresponds to the antibody in the well, or, if the sample contains an antibody that will bind the antigen or hapten in the well, an antigen-antibody complex will be formed. A detection compound is introduced and will recognize the formed complex and bind to the Fc portion of the antibody molecule. The detection compound is either labeled prior to the time of application or else is subsequently detected by radiolebeling, enzymatic or fluorescent reaction. Following serial washings, routine detection methods are employed.
Inventors
- 👤 Steven Hirschfeld
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 07:23
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S435/973; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/809; Y10S436/821
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
128 words
A method for assaying the presence or level of a multiplicity of antigens or haptens in a liquid complex mixture comprising the steps of: (a) adding the test sample containing the antigens or haptens to a solid phase matrix to form antigen-antibody complexes, said matrix having attached to it in identifiable positions antibodies or portions thereof, said antibodies or portions thereof directed against the antigens or haptens; (b) adding to the mixture formed in step (a) a known amount of labeled Detection Compound for antigen-antibody complexes; and (c) measuring the amount of labeled Detection Compound which remains bound to one or more of the identifiable positions of the solid phase matrix, and correlating that measurement with the presence or level of antigen or hapten in the identified positions.
Claim 2
Independent Claim
128 words
A method for assaying the presence or level of a multiplicity of antigens or haptens in a liquid complex mixture comprising the steps of: (a) adding the test sample containing the antigens or haptens to a solid phase matrix to form antigen-antibody complexes, said matrix having attached to it in identifiable positions antibodies or portions thereof, said antibodies or portions thereof directed against the antigens or haptens; (b) adding to the mixture formed in step (a) a known amount of labeled Detection Compound for antigen-antibody complexes; and (c) measuring the amount of labeled Detection Compound which remains bound to one or more of the identifiable positions of the solid phase matrix, and correlating that measurement with the presence or level of antigen or hapten in the identified positions.
Claim 3
Dependent Claim
23 words
The method of claim 1 wherein the labeling agent of the Detection Compound is radioactive element, fluorescent dye, an enzyme, or opaque particles.
Claim 4
Dependent Claim
17 words
The method of claim 1 wherein the Detection Compound is complement or the Clq component of complement.
Claim 5
Independent Claim
125 words
A method for assaying the presence or level of a multiplicity of antibodies in a liquid complex mixture comprising the steps of: (a) adding the test sample containing the antibodies or portions thereof to a solid phase matrix to form antigen-antibody complexes, said matrix having attached to it in identifiable positions antigens or haptens to which said antibodies, or portions thereof, can bind; (b) adding to the mixture formed in step (a) a known amount of labeled Detection Compound for antigen-antibody complexes; and (c) measuring the amount of labeled Detection Compound which remains bound to one or more of the identifiable positions of the solid phase matrix, and correlating that measurement with the presence or level of antibody or portion thereof in the identified positions.
Claim 6
Independent Claim
125 words
A method for assaying the presence or level of a multiplicity of antibodies in a liquid complex mixture comprising the steps of: (a) adding the test sample containing the antibodies or portions thereof to a solid phase matrix to form antigen-antibody complexes, said matrix having attached to it in identifiable positions antigens or haptens to which said antibodies, or portions thereof, can bind; (b) adding to the mixture formed in step (a) a known amount of labeled Detection Compound for antigen-antibody complexes; and (c) measuring the amount of labeled Detection Compound which remains bound to one or more of the identifiable positions of the solid phase matrix, and correlating that measurement with the presence or level of antibody or portion thereof in the identified positions.
Claim 7
Dependent Claim
23 words
The method of claim 4 wherein the labeling agent of the Detection Compound is radioactive element, fluorescent dye, an enzyme, or opaque particles.
Claim 8
Dependent Claim
17 words
The method of claim 4 wherein the Detection Compound is complement or the Clq component of complement.
Claim 9
Independent Claim
168 words
A method for assaying the presence or level of a multiplicity of antigens or haptens in a liquid complex mixture comprising the steps of: (a) adding the test sample containing the antigens or haptens to a solid phase matrix to form antigen-antibody complexes, said matrix having attached to it in identifiable positions antibodies or portions thereof, said antibodies or portions thereof directed against the antigens or haptens; (b) adding to the mixture formed in step (a) a known amount of Detection Compound for antigen-antibody complexes; (c) adding to the mixture formed in step (b) a labeled agent selected from the group consisting of a labeled antibody or portions thereof directed against the Detection Compound and a labeled component of complement which recognizes the Clq component of complement; and (d) measuring the amount of labeled agent which remains bound to one or more of the identifiable positions of the solid phase matrix, and correlating that measurement with the presence or level of antigen or hapten in the identified positions.
Claim 10
Independent Claim
168 words
A method for assaying the presence or level of a multiplicity of antigens or haptens in a liquid complex mixture comprising the steps of: (a) adding the test sample containing the antigens or haptens to a solid phase matrix to form antigen-antibody complexes, said matrix having attached to it in identifiable positions antibodies or portions thereof, said antibodies or portions thereof directed against the antigens or haptens; (b) adding to the mixture formed in step (a) a known amount of Detection Compound for antigen-antibody complexes; (c) adding to the mixture formed in step (b) a labeled agent selected from the group consisting of a labeled antibody or portions thereof directed against the Detection Compound and a labeled component of complement which recognizes the Clq component of complement; and (d) measuring the amount of labeled agent which remains bound to one or more of the identifiable positions of the solid phase matrix, and correlating that measurement with the presence or level of antigen or hapten in the identified positions.
Claim 11
Dependent Claim
36 words
The method of claim 7 wherein the Detection Compound added in step (b) is the Clq component of complement and adding in step (c) a labeled component of complement which recognizes the Clq component of complement.
Claim 12
Independent Claim
27 words
The method of claims 7 or 8 wherein the label of the labeled agent in step (c) is radioactive element, fluorescent dye, an enzyme, or opaque particles.
Claim 13
Independent Claim
19 words
The method of claims 1 or 7 wherein the test sample is a fluid or suspension of human origin.
Claim 14
Dependent Claim
34 words
The method of claim 10 wherein the fluid or suspension of human origin is serum, whole blood, ascitic fluid, urine, semen, milk, glandular secretions, bone marrow aspirate, or suspensions of whole or broken cells.
Claim 15
Independent Claim
165 words
A method for assaying the presence or level of a multiplicity of antibodies in a liquid complex mixture comprising the steps of: (a) adding the test sample containing the antibodies or portions thereof to a solid phase matrix to form antigen-antibody complexes, said matrix having attached to it in identifiable positions antigens or haptens to which said antibodies, or portions thereof, can bind; (b) adding to the mixture formed in step (a) a known amount of Detection Compound for antigen-antibody complexes; (c) adding to the mixture formed in step (b) a labeled agent selected from the group consisting of a labeled antibody or portions thereof directed against the Detection Compound and a labeled component of complement which recognizes the Clq component of complement; and (d) measuring the amount of labeled agent which remains bound to one or more of the identifiable positions of the solid phase matrix, and correlating that measurement with the presence or level of antibody or portion thereof in the identified positions.
Claim 16
Independent Claim
165 words
A method for assaying the presence or level of a multiplicity of antibodies in a liquid complex mixture comprising the steps of: (a) adding the test sample containing the antibodies or portions thereof to a solid phase matrix to form antigen-antibody complexes, said matrix having attached to it in identifiable positions antigens or haptens to which said antibodies, or portions thereof, can bind; (b) adding to the mixture formed in step (a) a known amount of Detection Compound for antigen-antibody complexes; (c) adding to the mixture formed in step (b) a labeled agent selected from the group consisting of a labeled antibody or portions thereof directed against the Detection Compound and a labeled component of complement which recognizes the Clq component of complement; and (d) measuring the amount of labeled agent which remains bound to one or more of the identifiable positions of the solid phase matrix, and correlating that measurement with the presence or level of antibody or portion thereof in the identified positions.
Claim 17
Dependent Claim
36 words
The method of claim 12 wherein the Detection Compound added in step (b) is the Clq component of complement and adding in step (c) a labeled component of complement which recognizes the Clq component of complement.
Claim 18
Independent Claim
27 words
The method of claims 12 or 13 wherein the label of the labeled agent in step (c) is radioactive element, fluorescent dye, an enzyme, or opaque particles.
Claim 19
Dependent Claim
19 words
The method of claim 4 or 12 wherein the test sample is a fluid or suspension of human origin.
Claim 20
Dependent Claim
34 words
The method of claim 15 wherein the fluid or suspension of human origin is serum, whole blood, ascitic fluid, urine, semen, milk, glandular secretions, bone marrow aspirate, or suspensions of whole or broken cells.
Claim 21
Dependent Claim
72 words
A diagnostic pack for the simultaneous detection and quantification of a multiplicity of antigens or haptens, antibodies or portions thereof, or a combination of antigens, haptens, antibodies, and portions of antibodies, comprising of: (a) a solid phase matix having either antigens or haptens, antibodies or portions thereof, or a combination of antigens, haptens, antibodies, and portions of antibodies attached in identifiable positions of the solid phase matrix; and (b) labeled Detection Compound.
Claim 22
Dependent Claim
72 words
A diagnostic pack for the simultaneous detection and quantification of a multiplicity of antigens or haptens, antibodies or portions thereof, or a combination of antigens, haptens, antibodies, and portions of antibodies, comprising of: (a) a solid phase matix having either antigens or haptens, antibodies or portions thereof, or a combination of antigens, haptens, antibodies, and portions of antibodies attached in identifiable positions of the solid phase matrix; and (b) labeled Detection Compound.
Claim 23
Dependent Claim
105 words
A diagnostic pack for the simultaneous detection and quantification of a multiplicity of antigens or haptens, antibodies or portions thereof, or a combination of antigens, haptens, antibodies, and portions of antibodies, comprising of: (a) a solid phase matrix having either antigens or haptens, antibodies or portions thereof, or a combination of antigens, haptens, antibodies, and portions of antibodies attached in identifiable positions of the solid phase matrix; (b) Detection Compound; and (c) a labeled agent selected from the group consisting of a labeled antibody or portions thereof directed against the Detection Compound and a labeled component of complement which recognizes the Clq component of complement.
Claim 24
Dependent Claim
105 words
A diagnostic pack for the simultaneous detection and quantification of a multiplicity of antigens or haptens, antibodies or portions thereof, or a combination of antigens, haptens, antibodies, and portions of antibodies, comprising of: (a) a solid phase matrix having either antigens or haptens, antibodies or portions thereof, or a combination of antigens, haptens, antibodies, and portions of antibodies attached in identifiable positions of the solid phase matrix; (b) Detection Compound; and (c) a labeled agent selected from the group consisting of a labeled antibody or portions thereof directed against the Detection Compound and a labeled component of complement which recognizes the Clq component of complement.
Claim 25
Dependent Claim
37 words
The diagnostic pack described in claim 18 wherein the Detection Compound in (b) is the Clq component of complement and the labeled agent in (c) is a component of complement which recognizes the Clq component of complement.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-29 | 1988-10-29 | 1989-04-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-29 | 1992-10-29 | 1993-04-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-29 | 1996-10-29 | 1997-04-30 | $7700.00 | Window Closed / Lapsed |