Patent US4513088A successfully refreshed from Google Patents.
US4513088A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Assay for monoclonal antibody against surface Ig of a human B cell tumor
Assignee: Leland Stanford Junior University
•
Filing: 1983-03-30
•
Granted: 1985-04-23
•
Expires: 2003-03-30
Abstract
Abstract An assay that facilitates screening of hybridoma culture media for monoclonal anti-idiotype antibodies, particularly murine monoclonal antibodies that are useful for treating human B cell tumors is disclosed. The assay is a solid phase type assay and involves: incubating a lysate of the patien REFERENCE TO GOVERNMENT GRANT OR CONTRACT The invention described herein was made in the course of work under a grant or contract from the National Institutes of Health.
Inventors
- 👤 Ronald Levy
- 👤 David G. Maloney
- 👤 Kristiaan Thielemans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 18:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6854; G01N33/686; G01N33/53; G01N33/575; G01N33/57557; G01N2333/00; G01N2333/435; G01N2333/705; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S435/965; Y10S435/969; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/813; Y10S436/823
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
173 words
An immunoassay for detecting a monoclonal antibody that binds specifically to the surface IG of a human B cell tumor in a hybridoma culture medium suspected of containing the monoclonal antibody comprising: (a) incubating a lysate of cells of the tumor with an antibody immobilized on a solid phase that binds to the surface Ig but not to the monoclonal antibody under conditions that promote binding of the surface Ig to the immobilized antibody; (b) removing unbound lysate material from the solid phase; (c) incubating the solid phase with the hybridoma culture medium under conditions that promote binding of any monoclonal antibody in the culture medium to immobilized antibody-surface Ig complex; (d) removing unbound culture medium material from the solid phase; (e) incubating the solid phase with a labeled antibody that binds to the monoclonal antibody under conditions that promote binding of the labeled antibody to immoblized antibody-surface Ig-monoclonal antibody complex; (f) removing unbound labeled antibody from the solid phase; (g) detecting the presence of labeled complex in the product of step (f).
Claim 2
Dependent Claim
173 words
An immunoassay for detecting a monoclonal antibody that binds specifically to the surface IG of a human B cell tumor in a hybridoma culture medium suspected of containing the monoclonal antibody comprising: (a) incubating a lysate of cells of the tumor with an antibody immobilized on a solid phase that binds to the surface Ig but not to the monoclonal antibody under conditions that promote binding of the surface Ig to the immobilized antibody; (b) removing unbound lysate material from the solid phase; (c) incubating the solid phase with the hybridoma culture medium under conditions that promote binding of any monoclonal antibody in the culture medium to immobilized antibody-surface Ig complex; (d) removing unbound culture medium material from the solid phase; (e) incubating the solid phase with a labeled antibody that binds to the monoclonal antibody under conditions that promote binding of the labeled antibody to immoblized antibody-surface Ig-monoclonal antibody complex; (f) removing unbound labeled antibody from the solid phase; (g) detecting the presence of labeled complex in the product of step (f).
Claim 3
Dependent Claim
23 words
The immunoassay of claim 1 wherein the monoclonal antibody is a murine monoclonal IgG antibody and the labeled antibody is an anti-mouse antibody.
Claim 4
Dependent Claim
18 words
The immunoassay of claim 2 wherein the immobilized antibody is one that does not bind to murine Ig.
Claim 5
Dependent Claim
12 words
The immunoassay of claim 3 wherein the immobilized antibody is goat antibody.
Claim 6
Dependent Claim
43 words
The immunoassay of claim 1 wherein the surface Ig is an IgM, the monoclonal antibody is a human IgG, the immobilized antibody is an anti-human IgM antibody and the labeled antibody is an anti-human IgG antibody that does not bind to human IgM.
Claim 7
Dependent Claim
27 words
The immunoassay of claim 1 wherein the material on which the immobilized antibody is immobilized is post-coated with a protein that does not bind to mammalian Ig.
Claim 8
Dependent Claim
19 words
The immunoassay of claim 1 wherein the labeled antibody is one that does not bind to the immobilized antibody.
Claim 9
Dependent Claim
14 words
The immunoassay of claim 1 wherein the labeled antibody is an enzyme labeled antibody.
Claim 10
Dependent Claim
59 words
The immunoassay of claim 2 wherein the immobilized antibody is one that does not bind to murine Ig, the material on which the antibody against human Ig is immobilized is post-coated with a protein that does bind to mammalian Ig, and the labeled antibody against mouse Ig is an enzyme labeled antibody that does not bind to human Ig.
Claim 11
Dependent Claim
17 words
The immunoassay of claim 9 wherein the immobilized antibody and the enzyme labeled antibody are goat antibodies.
References Cited (Backward Citations)
US4267270A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-22 | 1988-10-22 | 1989-04-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-22 | 1992-10-22 | 1993-04-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-22 | 1996-10-22 | 1997-04-23 | $7700.00 | Window Closed / Lapsed |