US4510245A
Expired / Lapsed
US
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Adenovirus promoter system
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1982-11-18
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Granted: 1985-04-09
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Expires: 2002-11-18
Abstract
Abstract The adenovirus major late promoter is employed as a promoter for expression in a yeast host. Constructions are provided for expression in yeast with the adenovirus major late promoter and a coding segment.
Inventors
- 👤 Lawrence S. Cousens
- 👤 Graeme I. Bell
- 👤 Pablo D. T. Valenzuela
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 21:39
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12N15/85; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
46 words
A yeast expression vector comprising a replication origin functional in yeast and a DNA coding segment and an adenovirus major late promoter segment adjacent to the 5' end of the coding segment, the promoter segment being oriented so as to initiate transcription of the coding segment.
Claim 2
Dependent Claim
46 words
A yeast expression vector comprising a replication origin functional in yeast and a DNA coding segment and an adenovirus major late promoter segment adjacent to the 5' end of the coding segment, the promoter segment being oriented so as to initiate transcription of the coding segment.
Claim 3
Dependent Claim
20 words
The expression vector of claim 1, comprising additionally a bacterial replication origin and being capable of being replicated in bacteria.
Claim 4
Dependent Claim
23 words
The vector of claim 1 or 2, comprising additionally a mammalian cell replication origin, and capable of being replicated in a mammalian cell.
Claim 5
Dependent Claim
19 words
The vector of claim 1, comprising additionally a transcription terminator segment adjacent the 3' end of the coding segment.
Claim 6
Independent Claim
114 words
A method of expressing a DNA coding segment in yeast, comprising the steps of (a) inserting the coding segment in a yeast expression vector, the vector comprising a replication origin functional in yeast and a DNA segment derived from an adenovirus major late promoter, the promoter being adjacent the 5' end of the inserted DNA coding segment and so oriented that transcription initiated within the adenovirus promoter segment includes the coding segment, thereby providing a coding segment expression vector, and (b) transforming a yeast cell with the coding segment expression vector, whereby synthesis of protein coded by the coding segment occurs during growth and replication of the transformed yeast cell under normal culture conditions.
Claim 7
Independent Claim
114 words
A method of expressing a DNA coding segment in yeast, comprising the steps of (a) inserting the coding segment in a yeast expression vector, the vector comprising a replication origin functional in yeast and a DNA segment derived from an adenovirus major late promoter, the promoter being adjacent the 5' end of the inserted DNA coding segment and so oriented that transcription initiated within the adenovirus promoter segment includes the coding segment, thereby providing a coding segment expression vector, and (b) transforming a yeast cell with the coding segment expression vector, whereby synthesis of protein coded by the coding segment occurs during growth and replication of the transformed yeast cell under normal culture conditions.
Claim 8
Dependent Claim
15 words
The method of claim 5, wherein the coding segment codes for hepatitis B surface antigen.
References Cited (Backward Citations)
US4387162A
Cited
US4430428A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-08 | 1988-10-08 | 1989-04-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-08 | 1992-10-08 | 1993-04-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-08 | 1996-10-08 | 1997-04-09 | $7700.00 | Window Closed / Lapsed |