US4508709A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for purifying factor VIII:C
Assignee: Armour Pharmaceutical Co
•
Filing: 1983-12-05
•
Granted: 1985-04-02
•
Expires: 2003-12-05
Abstract
Abstract Factor VIII:C is purified by chromatographic absorption on aminohexyl agarose at a pH above 5.5 up to about 8, and preferably 6.5 to 7.2, and a conductivity of about 25 to about 35 mS/cm.
Inventors
- 👤 Godfrey W. Amphlett
- 👤 Michael E. Hrinda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 04:25
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/14; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61P; A61P7/00; A61K; A61K38/00
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
82 words
A process for purifying Factor VIII:C from source material containing antihemophilic factor, comprising (a) providing an aqueous solution of said source material which has a pH of 6.0 to about 8.0, and a conductivity of about 25 mS/cm to 35 mS/cm, (b) equilibrating aminohexyl agarose to the pH of said solution, (c) adsorbing antihemophilic factor from said solution onto said equilibrated aminohexyl agarose, (d) eluting Factor VIII:R from the aminohexyl agarose, and then (e) eluting the Factor VIII:C from the aminohexyl agarose.
Claim 2
Dependent Claim
82 words
A process for purifying Factor VIII:C from source material containing antihemophilic factor, comprising (a) providing an aqueous solution of said source material which has a pH of 6.0 to about 8.0, and a conductivity of about 25 mS/cm to 35 mS/cm, (b) equilibrating aminohexyl agarose to the pH of said solution, (c) adsorbing antihemophilic factor from said solution onto said equilibrated aminohexyl agarose, (d) eluting Factor VIII:R from the aminohexyl agarose, and then (e) eluting the Factor VIII:C from the aminohexyl agarose.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein the pH of said source material is 6.0 to 7.5.
Claim 4
Dependent Claim
16 words
The process of claim 1 wherein the pH of said source material is 6.5 to 7.2.
Claim 5
Dependent Claim
20 words
The process of claim 1 wherein the aminohexyl agarose contains at least about 10 micromoles of aminohexyl chains per gram.
Claim 6
Dependent Claim
14 words
The process of claim 1 wherein the source material is plasma which contains AHF.
Claim 7
Dependent Claim
16 words
The process of claim 1 wherein the source material is a plasma concentrate which contains AHF.
Claim 8
Dependent Claim
15 words
The process of claim 1 wherein the source material is a cryoprecipitate which contains AHF.
Claim 9
Dependent Claim
21 words
The process of claim 1 wherein the source material also contains fibronectin, and the fibronectin is eluted with the Factor VIII:R.
Claim 10
Dependent Claim
21 words
The process of claim 1 wherein the source material also contains fibrinogen, and the fibrinogen is eluted with the Factor VIII:R.
Claim 11
Dependent Claim
19 words
The process of claim 1 wherein the Factor VIII:C is purified with a yield of at least about 30%.
Claim 12
Dependent Claim
46 words
The process of claim 1 wherein the Factor VIII:C is purified with a yield of at least about 50%, and wherein a proteolytic inhibitor is present in steps (a), (b), (c) and (d) in a small but effective amount to inhibit proteolysis of the Factor VIII:C.
Claim 13
Dependent Claim
26 words
The process of claim 1 wherein the Factor VIII:C is purified by a factor of at least about 10 times its purity in the source material.
Claim 14
Dependent Claim
26 words
The process of claim 1 wherein the Factor VIII:C is purified by a factor of at least about 20 times its purity in the source material.
Claim 15
Dependent Claim
20 words
The process of claim 1 wherein the Factor VIII:C is eluted with a buffered aqueous solution containing 1.0 M NaCl.
Claim 16
Dependent Claim
24 words
The process of claim 1 wherein the Factor VIII:C is eluted with an aqueous buffered solution containing 0.3 to 0.5 M CaCl 2 .
Claim 17
Dependent Claim
74 words
The process for purifying Factor VIII:C from source material containing antihemophilic factor, comprising (a) providing an aqueous solution of said source material which has a pH of 6.0 to about 8.0 and a conductivity of about 25 mS/cm to 35 mS/cm, (b) equilibrating aminohexyl agarose to the pH of said solution, (c) adsorbing antihemophilic factor from said solution onto said equilibrated aminohexyl agarose, and then (d) eluting the Factor VIII:C from the aminohexyl agarose.
Claim 18
Dependent Claim
74 words
The process for purifying Factor VIII:C from source material containing antihemophilic factor, comprising (a) providing an aqueous solution of said source material which has a pH of 6.0 to about 8.0 and a conductivity of about 25 mS/cm to 35 mS/cm, (b) equilibrating aminohexyl agarose to the pH of said solution, (c) adsorbing antihemophilic factor from said solution onto said equilibrated aminohexyl agarose, and then (d) eluting the Factor VIII:C from the aminohexyl agarose.
Claim 19
Dependent Claim
15 words
The process of claim 16 wherein the source material is plasma which contains antihemophilic factor.
Claim 20
Dependent Claim
17 words
The process of claim 16 wherein the source material is a plasma concentrate which contains antihemophilic factor.
Claim 21
Dependent Claim
16 words
The process of claim 16 wherein the source material is a cryoprecipitate which contains antihemophilic factor.
Claim 22
Dependent Claim
20 words
The process of claim 16 wherein the pH of the aqueous solution provided in step (a) is 6.0 to 7.5.
Claim 23
Dependent Claim
20 words
The process of claim 16 wherein the pH of the aqueous solution provided in step (a) is 6.5 to 7.2.
References Cited (Backward Citations)
US4361509A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-01 | 1988-10-01 | 1989-04-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-01 | 1992-10-01 | 1993-04-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-01 | 1996-10-01 | 1997-04-02 | $7700.00 | Window Closed / Lapsed |