Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1

Claim 2

A murine-derived hybridoma cell line capable of producing in the medium of its growth a monoclonal antibody capable of specifically binding with Colony Stimulating Factor Subclass Number 1 in an antigen/antibody complex.

Claim 3

A hybridoma cell line according to claim 1 which is ATCC No. HB8207.

Claim 4

A hybridoma cell line according to claim 1 which is ATCC No. HB8208.

Claim 5

A monoclonal antibody produced by a hybridoma cell line according to claim 1.

Claim 6

A monoclonal antibody produced by hybridoma cell line ATCC No. HB8207 according to claim 2.

Claim 7

A monoclonal antibody produced by hybridoma cell line ATCC No. HB8208 according to claim 3.

Claim 8

In an immunological procedure for isolation of biologically active Colony Stimulating Factor Subclass Number 1 from a biological fluid on the basis of a selective immunological reaction with an antibody specific for Colony Stimulating Factor Subclass Number 1, the improvement comprising: employing the monoclonal antibody of claim 4 or 5 or 6 as said specific antibody.

Claim 9

The improvement of claim 7 wherein said fluid is a human fluid.

Claim 10

The improvement of claim 7 wherein said fluid is a member of the group comprising whole blood, serum and urine.

Claim 11

In an immunological procedure for the quantitative detection of Colony Stimulating Factor Subclass Number 1 in a biological fluid on the basis of a selective immunological reaction with an antibody specific for Colony Stimulating Factor Subclass Number 1, the improvement comprising: employing the monoclonal antibody of claim 4 or 5 or 6 as said specific antibody.

Claim 12

The improvement of claim 10 wherein said fluid is a human fluid.

Claim 13

The improvement of claim 10 wherein said fluid is a member of the group comprising whole blood, serum and urine.

Claim 14

The improvement of claim 10 wherein said immunological procedure is a radioimmunobinding assay.

Claim 15

A monoclonal antibody produced by a murine-derived hybridoma cell line according to claim 1, said antibody capable of specifically binding in an antigen-antibody complex with that portion of a polypeptide which consists essentially of an amino acid sequence comprising: NHR-Asp-Val-Glu-Phe-Asp-COR', wherein R and R' are the same or different amino acid residues or R is hydrogen or R' is a hydroxyl group.

Claim 16

The product according to claim 14 wherein the amino acid sequence of the polypeptide comprises: RNH-Leu-Pro-Pro-Asp-Val-Glu-Phe-Asp-Arg-Pro-Phe-Leu-Arg-COR', wherein R and R' are the same or different amino acid residues or R is hydrogen or R' is a hydroxyl group.

Claim 17

The product of claim 15 wherein R is selected from the group consisting of serine, histidine, and glutamic acid and R' is cystein.

Claim 18

An immunological assay for quantitative detection of Colony Stimulating Factor Subclass Number 1 in a biological fluid sample comprising the steps of: (1) contacting said fluid with a first, immobilized, antibody which reacts with a first antigenic determinant of Colony Stimulating Factor Subclass Number 1 in said fluid to form an immunological complex of Colony Stimulating Factor Subclass Number 1 and said first antibody; (2) contacting the complex formed in step (1) with a second antibody which reacts with an antigenic determinant of Colony Stimulating Factor Subclass Number 1 other than said first antigenic determinant, to form an immunological complex of Colony Stimulating Factor Subclass Number 1 and said second antibody; and (3) quantifying the amount of said second antibody bound to said immunological complex formed in step (2).

Claim 19

An immunological assay for quantitative detection of Colony Stimulating Factor Subclass Number 1 in a biological fluid sample comprising the steps of: (1) contacting said fluid with a first, immobilized, antibody which reacts with a first antigenic determinant of Colony Stimulating Factor Subclass Number 1 in said fluid to form an immunological complex of Colony Stimulating Factor Subclass Number 1 and said first antibody; (2) contacting the complex formed in step (1) with a second antibody which reacts with an antigenic determinant of Colony Stimulating Factor Subclass Number 1 other than said first antigenic determinant, to form an immunological complex of Colony Stimulating Factor Subclass Number 1 and said second antibody; and (3) quantifying the amount of said second antibody bound to said immunological complex formed in step (2).

Claim 20

The assay according to claim 17 wherein said first antibody is a monoclonal antibody produced by a murine-derived hybridoma cell line capable of producing in the medium of its growth a monoclonal antibody capable of specifically binding with Colony Stimulating Factor Subclass Number 1 in an antigen/antibody complex and said second antibody is selected from the group consisting of a serum-derived polyvalent antibody to Colony Stimulating Factor Subclass Number 1, or a monoclonal antibody produced by hybridoma cell line ATCC No. HB8207, or a monoclonal antibody produced by hybridoma cell line ATCC No. HB8208 or a monoclonal antibody produced by a murine-derived hybridoma cell line capable of producing in the medium of its growth a monoclonal antibody capable of specifically binding with Colony Stimulating Factor Subclass Number 1 in an antigen/antibody complex, said antibody capable of specifically binding in an antigen/antibody complex with that portion of a polypeptide which consists essentially of an amino acid sequence comprising: NHR-Asp-Val-Glu-Phe-Asp-COR', wherein R and R' are the same or different amino acid residues or R is hydrogen or R' is a hydroxyl group.

Claim 21

The assay according to claim 17 wherein said first antibody is a monoclonal antibody produced by hybridoma cell line ATCC No. HB8207, and said second antibody is selected from the group consisting of a serum-derived polyvalent antibody to Colony Stimulating Factor Subclass Number 1, or a monoclonal antibody produced by a murine-derived hybridoma cell line capable of producing in the medium of its growth a monoclonal antibody capable of specifically binding with Colony Stimulating Factor Subclass Number 1 in an antigen/antibody complex, or a monoclonal antibody produced by hybridoma cell line ATCC No. HB8208, or a monoclonal antibody produced by a murine-derived hybridoma cell line capable of producing in the medium of its growth a monoclonal antibody capable of specifically binding with Colony Stimulating Factor Subclass Number 1 in an antigen/antibody complex, said antibody capable of specifically binding in an antigen/antibody complex with that portion of a polypeptide which consists essentially of an amino acid sequence comprising: NHR-Asp-Val-Glu-Phe-Asp-COR', wherein R and R' are the same or different amino acid residues or R is hydrogen or R' is a hydroxyl group.

Claim 22

The assay according to claim 17 wherein said first antibody is a monoclonal antibody produced by hybridoma cell line ATCC No. HB8208, and said second antibody is selected from the group consisting of a serum-derived polyvalent antibody to Colony Stimulating Factor Subclass Number 1, or a monoclonal antibody produced by a murine-derived hybridoma cell line capable of producing in the medium of its growth a monoclonal antibody capable of specifically binding with Colony Stimulating Factor Subclass Number 1 in an antigen/antibody complex, or a monoclonal antibody produced by hybridoma cell line ATCC No. HB8207 or a monoclonal antibody produced by a murine-derived hybridoma cell line capable of producing in the medium of its growth a monoclonal antibody capable of specifically binding with Colony Stimulating Factor Subclass Number 1 in an antigen/antibody complex, said antibody capable of specifically binding in an antigen/antibody complex with that portion of a polypeptide which consists essentially of an amino acid sequence comprising: NHR-Asp-Val-Glu-Phe-Asp-COR', wherein R and R' are the same or different amino acid residues or R is hydrogen or R' is a hydroxyl group.

Claim 23

The assay according to claim 17 wherein said first antibody is a monoclonal antibody produced by a murine-derived hybridoma cell line capable of producing in the medium of its growth a monoclonal antibody capable of specifically binding with Colony Stimulating Factor Subclass Number 1 in an antigen/antibody complex, said antibody capable of specifically binding in an antigen/antibody complex with that portion of a polypeptide which consists essentially of an amino acid sequence comprising: NHR-Asp-Val-Glu-Phe-Asp-COR', wherein R and R' are the same or different amino acid residues or R is hydrogen or R' is a hydroxyl group, and said second antibody is selected from the group consisting of a serum-derived polyvalent antibody to Colony Stimulating Factor Subclass Number 1, or a monoclonal antibody produced by a murine-derived hybridoma cell line capable of producing in the medium of its growth a monoclonal antibody capable of specifically binding with Colony Stimulating Factor Subclass Number 1 in an antigen/antibody complex, or a monoclonal antibody produced by hybridoma cell line ATCC No. HB8207 or a monoclonal antibody produced by hybridoma cell line ATCC No. HB8208.