Patent US3279030A successfully refreshed from Google Patents.
Patent US4504547A successfully refreshed from Google Patents.
US4504547A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing supports for cell cultures and supports so obtained
Assignee: Institut Pasteur
•
Filing: 1979-11-13
•
Granted: 1985-03-12
•
Expires: 1999-11-13
Abstract
Abstract The present invention relates to the use of thermosetting plastics for improving supports used for cell growth. The process according to the invention consists of applying to base supports, a solution of a thermosetting plastics material and of then subjecting the thus-coated supports to the effect of heat under conditions sufficient to permit the hardening of the thermosetting plastics material and sterilization of the thus-coated supports. The supports according to the invention are suitable for any conventional seeding and trypsination operations on cell cultures; they can also be used for the cultivation of human or animal diploid cells.
Inventors
- 👤 Florian Horodniceanu
- 👤 Raphael Le Fur
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 19:14
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N5/0075; C12N2533/00; C12N2533/30; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2998; Y10T428/31504; Y10T428/31551; Y10T428/31627; Y10T428/3163; Y10T428/31645; Y10T428/31971
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
62 words
Process for producing supports for cell cultures, comprising applying to glass or polysaccharide base supports a solution of a thermosetting plastic material comprising the reaction product of an aldehyde with a polyvinyl alcohol and then subjecting said support thus-coated to the effect of heat under sufficient conditions to permit hardening of said thermosetting plastic material and the sterilization of said thus-coated supports.
Claim 2
Dependent Claim
62 words
Process for producing supports for cell cultures, comprising applying to glass or polysaccharide base supports a solution of a thermosetting plastic material comprising the reaction product of an aldehyde with a polyvinyl alcohol and then subjecting said support thus-coated to the effect of heat under sufficient conditions to permit hardening of said thermosetting plastic material and the sterilization of said thus-coated supports.
Claim 3
Dependent Claim
25 words
Process according to claim 1, wherein the solution of thermosetting plastic material is applied to the surface or surfaces that will contact the cell cultures.
Claim 4
Dependent Claim
52 words
Process according to claim 1, wherein the thermosetting plastics material is a polyvinyl butyral polymer resulting from the reaction of butyraldehyde with a polyvinyl alcohol whose viscosity of 20° C. of a 5% solution in 95° ethyl alcohol is 10 millipoises and whose percentage by weight of polyvinyl alcohol groups is 20.
Claim 5
Dependent Claim
17 words
Process according to claim 1, wherein the base supports are Petri dishes, Roux dishes or "DEAE-Sephadex" beads.
Claim 6
Dependent Claim
30 words
Process according to claim 1, wherein said support coated with the thermosetting plastics solution is subjected to the effect of heat at a temperature comprised between 120° and 180° C.
Claim 7
Dependent Claim
30 words
Process according to claim 1, wherein, before the application of the thermosetting plastics solution, the supports are washed by means of an isotonic buffer and then passed into an autoclave.
Claim 8
Dependent Claim
44 words
Process according to claim 1, wherein the base supports are beads or microsupports and, after heat treatment in an autoclave, the beads or microsupports are rinsed with an isotonic buffer containing Ca ++ and Mg ++ ions and passed into the autoclave for sterilization.
Claim 9
Dependent Claim
12 words
Supports for cell cultures obtained by the process according to claim 1.
Claim 10
Dependent Claim
25 words
Supports for cell cultures according to claim 8, wherein the base support has a flat shape or a spherical shape and bears a thermoset coating.
Claim 11
Dependent Claim
19 words
Supports according to claim 8, wherein the thermoset coating is constituted by a polyvinyl butyral or polyvinyl formal polymer.
Claim 12
Dependent Claim
54 words
Supports according to claim 9, wherein the thermoset coating is constituted by a polymer of polyvinyl butyral resulting from the reaction of butyraldehyde with a polyvinyl alcohol whose viscosity at 20° C. of a 5% solution in 95° ethyl alcohol is 10 millipoises and whose percentage by weight of polyvinyl alcohol groups is 20.
References Cited (Backward Citations)
US3097070A
Cited
US3102082A
Cited
US3282722A
Cited
US3437553A
Cited
US3532605A
Cited
US3746196A
Cited
US3767790A
Cited
US3860490A
Cited
US3910819A
Cited
US4036693A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-03-11 | 1988-09-10 | 1989-03-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-03-11 | 1992-09-10 | 1993-03-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-03-11 | 1996-09-10 | 1997-03-12 | $7700.00 | Window Closed / Lapsed |