Patent US4497710A successfully refreshed from Google Patents.
US4497710A
Expired / Lapsed
US
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Substrate for liquid chromatography and process for isolating and purifying fat-soluble substance by the liquid chromatography on the substrate
Assignee: Kureha Corp
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Filing: 1982-09-30
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Granted: 1985-02-05
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Expires: 2002-09-30
Abstract
Abstract A substrate for liquid chromatography comprising a spherical particle of highly cross-linked polymer which is produced from (a) 100 parts by weight of a monomer of vinyl ester, acrylate or methacrylate, these esters having a long alkyl chain, if desired, together with a comonomer copolymerizable with the monomer and (b) 10 to 80 parts by weight of a cross-linking agent copolymerizable with the monomer and having at least two functional groups, and a process for isolating and purifying a fat-soluble substance, for instance, a lower alkyl ester of eicosapentaenoic acid or docosahexaenoic acid by means of the liquid chromatography on the substrate.
Inventors
- 👤 Masakatsu Wagu
- 👤 Shoichiro Hayashi
- 👤 Naohiro Murayama
- 👤 Teruo Sakagami
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:35
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/22; B01J20/26; B01D; B01D15/00; B01D15/08
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
95 words
A process for isolating and purifying a fat-soluble substance comprising subjecting the fat-soluble substance to liquid chromatography by using as a substrate spherical particles of a highly cross-linked polymer which comprises: (a) 100 parts by weight of at least one monomer unit selected from the group consisting of vinyl octanoate, vinyl laurate, vinyl stearate, 2-ethylhexyl acrylate, 2-ethylhexyl methacrylate, dodecyl acrylate, dodecyl methacrylate, stearyl acrylate and stearyl methacrylate, and (b) 10 to 100 parts by weight of a cross-linking agent unit which is copolymerizable with any of said monomer and has at least two functional groups.
Claim 2
Dependent Claim
95 words
A process for isolating and purifying a fat-soluble substance comprising subjecting the fat-soluble substance to liquid chromatography by using as a substrate spherical particles of a highly cross-linked polymer which comprises: (a) 100 parts by weight of at least one monomer unit selected from the group consisting of vinyl octanoate, vinyl laurate, vinyl stearate, 2-ethylhexyl acrylate, 2-ethylhexyl methacrylate, dodecyl acrylate, dodecyl methacrylate, stearyl acrylate and stearyl methacrylate, and (b) 10 to 100 parts by weight of a cross-linking agent unit which is copolymerizable with any of said monomer and has at least two functional groups.
Claim 3
Dependent Claim
22 words
The process according to claim 1, wherein the diameter of said spherical particles is in the range of 3 to 500 microns.
Claim 4
Dependent Claim
15 words
The process according to claim 1, wherein said monomer is vinyl stearate or stearyl methacrylate.
Claim 5
Dependent Claim
50 words
The process according to claim 1, wherein said cross-linking agent is trifunctional and has the formula (I) or (II): ##STR3## wherein R 1 , R 2 and R 3 are the same or different from each other and are selected from the group consisting of allyl, 2-propynyl and 2-methyl-2-propenyl groups.
Claim 6
Dependent Claim
16 words
The process according to claim 1, wherein said fat-soluble substance is a lower-alkyl eicosapentaenoate or dodecosahexaenoate.
Claim 7
Dependent Claim
60 words
The process according to claim 1, wherein said fat-soluble substance is selected from the group consisting of a glyceride having a saturated or unsaturated alkyl group or groups; a fat-soluble vitamin selected from the group of vitamins A, D and E; a steroid and a derivative thereof; and an aromatic-, polycyclic aromatic- or heterocyclic aromatic compound and a derivative thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-02-05 | 1988-08-06 | 1989-02-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-02-05 | 1992-08-06 | 1993-02-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-02-05 | 1996-08-06 | 1997-02-05 | $7700.00 | Window Closed / Lapsed |