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US4490473A
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Labeled antibodies and methods
Assignee: PANAB A CA CORP
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Filing: 1983-03-28
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Granted: 1984-12-25
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Expires: 2003-03-28
Abstract
Abstract Sandwich immunoassays are provided wherein an anti-analyte antibody is coupled with a detector labeled antibody, the anti-analyte antibody being substituted with a N,N,N-trimethylammoniumphenyl group and the detector labeled antibody is an antibody specific to the N,N,N-trimethylammoniumphenyl group. The invention includes novel assays, antigens and antibodies.
Inventors
- 👤 Robert F. Brunhouse
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 07:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/822; Y10S530/00; Y10S530/806; Y10S530/808; Y10S530/809; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
105 words
A sandwich immunoassay method comprising coupling an N,N,N-trimethylammoniumphenyl substituted antibody of the formula: ##STR7## wherein A 1 is an antibody bonded through an amino linkage thereof; R is methyl or ethyl; X is nitro, iodo, bromo, methoxy, carboxy or acetyl; m is 0, 1 or 2; and n is from 1 to 350; with labeled antibody prepared in immunological response in a mammal to antigenic compound of the formula: ##STR8## wherein A 2 is an antigen; R 1 is methyl or ethyl; X 1 is nitro, iodo, bromo, methoxy, carboxy or acetyl; p is 0, 1 or 2; and q is from 1 to 350.
Claim 2
Dependent Claim
105 words
A sandwich immunoassay method comprising coupling an N,N,N-trimethylammoniumphenyl substituted antibody of the formula: ##STR7## wherein A 1 is an antibody bonded through an amino linkage thereof; R is methyl or ethyl; X is nitro, iodo, bromo, methoxy, carboxy or acetyl; m is 0, 1 or 2; and n is from 1 to 350; with labeled antibody prepared in immunological response in a mammal to antigenic compound of the formula: ##STR8## wherein A 2 is an antigen; R 1 is methyl or ethyl; X 1 is nitro, iodo, bromo, methoxy, carboxy or acetyl; p is 0, 1 or 2; and q is from 1 to 350.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein R,X, and m are the same as R 1 , X 1 and p, respectively.
Claim 4
Dependent Claim
17 words
The sandwich immunoassay method of claim 1 wherein the compound of Formula I is 2-nitro-4-(N,N,N-trimethylammonium)phenyl substituted antibody.
Claim 5
Dependent Claim
29 words
The sandwich immunoassay method of claim 3 wherein the antigenic compound of Formula II is 2-nitro-4-(N,N,N-trimethylammonium)phenyl substituted bovine serum albumin conjugated through amine groups of the bovine serum albumin.
Claim 6
Dependent Claim
47 words
A N,N,N-trimethylammoniumphenyl substituted antibody of the formula: ##STR9## wherein A 1 is an antibody bonded through an amino linkage thereof; R is methyl or ethyl; X is nitro, iodo, bromo, methoxy, carboxy or acetyl; m is 0, 1 or 2; and n is from 1 to 350.
Claim 7
Dependent Claim
47 words
A N,N,N-trimethylammoniumphenyl substituted antibody of the formula: ##STR9## wherein A 1 is an antibody bonded through an amino linkage thereof; R is methyl or ethyl; X is nitro, iodo, bromo, methoxy, carboxy or acetyl; m is 0, 1 or 2; and n is from 1 to 350.
Claim 8
Dependent Claim
10 words
As an antibody compound of claim 5, 2-nitro-4-(N,N,N-trimethylammonium)phenyl substituted antibody.
Claim 9
Dependent Claim
65 words
An antibody prepared in immunological response in a mammal to a compound of the formula: ##STR10## wherein A 2 is an antigen bonded through an amino linkage thereof; R 1 is methyl or ethyl; X 1 is nitro, iodo, bromo, methoxy, carboxy or acetyl; p is 0, 1 or 2; and q is from 1 to 350; which specifically binds with compounds of claim 5.
Claim 10
Dependent Claim
15 words
An antibody of claim 7 prepared in immunological response to 2-nitro-4-(N,N,N-trimethylammonium)phenyl substituted bovine serum albumin.
Claim 11
Dependent Claim
19 words
The antibody of claim 7 having a detector label selected from the group of radioactive, enzymatic and fluorescent labels.
Claim 12
Dependent Claim
35 words
A N,N,N-trimethylammoniumbenzene compound of the formula: ##STR11## wherein R 2 is methyl or ethyl; X 2 is nitro, iodo, bromo, methoxy, carboxy or acetyl; Y 1 is sulfonyl; and r is 0, 1 or 2.
Claim 13
Dependent Claim
35 words
A N,N,N-trimethylammoniumbenzene compound of the formula: ##STR11## wherein R 2 is methyl or ethyl; X 2 is nitro, iodo, bromo, methoxy, carboxy or acetyl; Y 1 is sulfonyl; and r is 0, 1 or 2.
Claim 14
Dependent Claim
62 words
An antigenic compound of the formula: ##STR12## wherein A 2 is an antigen bonded through an amino linkage thereof; R 1 is methyl or ethyl; X 1 is nitro, iodo, bromo, methoxy, carboxy or acetyl; p is 0, 1 or 2; and q is from 1 to 350; with the proviso that when X 1 is nitro, A 2 is not insulin.
Claim 15
Dependent Claim
62 words
An antigenic compound of the formula: ##STR12## wherein A 2 is an antigen bonded through an amino linkage thereof; R 1 is methyl or ethyl; X 1 is nitro, iodo, bromo, methoxy, carboxy or acetyl; p is 0, 1 or 2; and q is from 1 to 350; with the proviso that when X 1 is nitro, A 2 is not insulin.
References Cited (Backward Citations)
No citation records indexed.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1987-12-25 | 1988-06-25 | 1988-12-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-25 | 1992-06-25 | 1992-12-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-25 | 1996-06-25 | 1996-12-25 | $7700.00 | Window Closed / Lapsed |