Patent US4489160A successfully refreshed from Google Patents.
US4489160A
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Plasmid pCG2
Assignee: KH Neochem Co Ltd
•
Filing: 1982-08-24
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Granted: 1984-12-18
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Expires: 2002-08-24
Abstract
Abstract Disclosed is a novel vector, plasmid pCG2, and a process of producing plasmid pCG2 from Corynebacterium glutamicum.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:18
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
46 words
An isolated plasmid pCG2 characterized by a molecular weight of about 6.6 kilobases and the following cleavage sites for restriction endonucleases: ______________________________________
Restriction Number of
enzyme cleavage sites
______________________________________
HindIII 3
KpnI 2
BamHI 1
PstI 1
HpaI 1
EcoRI 0
SalI 0
BglII 0
______________________________________
Claim 2
Dependent Claim
46 words
An isolated plasmid pCG2 characterized by a molecular weight of about 6.6 kilobases and the following cleavage sites for restriction endonucleases: ______________________________________
Restriction Number of
enzyme cleavage sites
______________________________________
HindIII 3
KpnI 2
BamHI 1
PstI 1
HpaI 1
EcoRI 0
SalI 0
BglII 0
______________________________________
Claim 3
Dependent Claim
28 words
A process for producing plasmid pCG2 which comprisses culturing Corynebacterium glutamicum 225-218 in a nutrient medium, disrupting the cultured cells and recovering plasmid pCG2 from the disrupted cells.
Claim 4
Dependent Claim
28 words
A process for producing plasmid pCG2 which comprisses culturing Corynebacterium glutamicum 225-218 in a nutrient medium, disrupting the cultured cells and recovering plasmid pCG2 from the disrupted cells.
Claim 5
Dependent Claim
17 words
A biologically pure culture of a microorganism belonging to the species Corynebacterium glutamicum and carrying plasmid pCG2.
Claim 6
Dependent Claim
17 words
A biologically pure culture of a microorganism belonging to the species Corynebacterium glutamicum and carrying plasmid pCG2.
Claim 7
Dependent Claim
18 words
A biologically pure culture of a microorganism according to claim 3, which is Corynebacterium glutamicum 225-218, ATCC 31832.
Claim 8
Dependent Claim
20 words
A biologically pure culture of a microorganism belonging to Corynebacterium glutamicum which carries plasmid pCG2 isolated from Corynebacterium glutamicum 225-218.
Claim 9
Dependent Claim
20 words
A biologically pure culture of a microorganism belonging to Corynebacterium glutamicum which carries plasmid pCG2 isolated from Corynebacterium glutamicum 225-218.
Claim 10
Dependent Claim
38 words
A recombinant plasmid constructed from the plasmid pCG2 defined in claim 1 by deleting a DNA region from said plasmid that does not affect its function to replicate autonomously or adding a foreign DNA fragment to said plasmid.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-12-18 | 1988-06-18 | 1988-12-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-18 | 1992-06-18 | 1992-12-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-18 | 1996-06-18 | 1996-12-18 | $7700.00 | Window Closed / Lapsed |