Patent US4488991A successfully refreshed from Google Patents.
US4488991A
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Bacterial toxoids and gram-negative immune globulin therefrom
Assignee: Merck and Co Inc
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Filing: 1983-10-26
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Granted: 1984-12-18
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Expires: 2003-10-26
Abstract
Abstract ADP-Ribosylating toxins are rendered enzymatically inactive by reactions with photolabile affinity reagents. The toxoids retain the antigenic and immunogenic properties of the original toxins. These bacterial toxoids can be used as immunogens to protect against the specific disease that the precursor toxins cause or, in the case of P. aeruginosa, the toxoid can be used in combination with E. coli J-5 vaccine to protect against gram-negative bacteremia in general.
Inventors
- 👤 Richard L. Tolman
- 👤 Stephen Marburg
- 👤 Lynn T. Callahan, III
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 11:44
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/12; A; A61; A61K; A61K39/00; A61K39/02; A61K39/104; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/807; Y10S530/82; Y10S530/825
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
49 words
A process for preparing toxoids which comprises: (1) mixing and ADP-ribosylating toxin with a photolabile affinity reagent selected from the group consisting of 8-azidoadenosine and 8-azidoadenine, at about 0° C. under an inert atmosphere, and (2) exposing the mixture of step 1 to an effective amount of nondenaturing light.
Claim 2
Dependent Claim
49 words
A process for preparing toxoids which comprises: (1) mixing and ADP-ribosylating toxin with a photolabile affinity reagent selected from the group consisting of 8-azidoadenosine and 8-azidoadenine, at about 0° C. under an inert atmosphere, and (2) exposing the mixture of step 1 to an effective amount of nondenaturing light.
Claim 3
Dependent Claim
33 words
The process of claim 1 wherein the toxin is P. aeruginosa exotoxin-A, the photolabile affinity reagent is 8-azidoadenosine and the molar ratio of exotoxin to photolabile affinity reagent ranges from 1:100 to 1:10,000.
Claim 4
Dependent Claim
16 words
The process of claim 1, also comprising (3) desalting the the toxoid product of step 2.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-12-18 | 1988-06-18 | 1988-12-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-18 | 1992-06-18 | 1992-12-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-18 | 1996-06-18 | 1996-12-18 | $7700.00 | Window Closed / Lapsed |