Patent US4487829A successfully refreshed from Google Patents.
US4487829A
Expired / Lapsed
US
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Production and use of monoclonal antibodies against adenoviruses
Assignee: Massachusetts Institute of Technology
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Filing: 1982-03-23
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Granted: 1984-12-11
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Expires: 2002-03-23
Abstract
Abstract Monoclonal antibodies, and a cell line characterized by its production of such monoclonal antibodies, demonstrating specific reactivity to an antigenic determinant possessed by a plurality of types of adenoviruses, a method of isolating such cell lines, and the use of such antibodies for diagnostic and therapeutic purposes as well as for identifying chemical compounds with similar properties, are disclosed.
Inventors
- 👤 Phillip A. Sharp
- 👤 Constance L. Cepko
- 👤 Paul Changelian
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 11:53
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/08; C07K16/081; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S530/00; Y10S530/808; Y10S530/809
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
50 words
A monoclonal antibody demonstrating specific reactivity to an antigenic determinant possessed by a plurality of types of adenoviruses, the antibody characterized further by its reactivity with hexon trimers derived from adenoviruses and its lack of reactivity in immunoprecipitation reactions with intact adenovirus virions, denatured hexon polypeptides and monomeric hexon polypeptides.
Claim 2
Dependent Claim
50 words
A monoclonal antibody demonstrating specific reactivity to an antigenic determinant possessed by a plurality of types of adenoviruses, the antibody characterized further by its reactivity with hexon trimers derived from adenoviruses and its lack of reactivity in immunoprecipitation reactions with intact adenovirus virions, denatured hexon polypeptides and monomeric hexon polypeptides.
Claim 3
Dependent Claim
27 words
The monoclonal antibody according to claim 1 and further characterized by its positive immunoprecipitation of hexon trimers derived from capsomers of human Ad2 and human Ad5 adenoviruses.
Claim 4
Dependent Claim
61 words
A monoclonal antibody demonstrating: a. positive reaction to hexon trimer; b. lack of reactivity with denatured or monomeric hexon; c. inability to immunoprecipitate intact adenovirus virions; d. lack of adenovirus neutralizing activity; e. nuclear immunofluorescence in cells where adenoviruses are present; f. reactivity with human subgroups A-E, simian, bovine, canine and swine adenoviruses; and g. lack of reactivity with avian adenovirus.
Claim 5
Dependent Claim
61 words
A monoclonal antibody demonstrating: a. positive reaction to hexon trimer; b. lack of reactivity with denatured or monomeric hexon; c. inability to immunoprecipitate intact adenovirus virions; d. lack of adenovirus neutralizing activity; e. nuclear immunofluorescence in cells where adenoviruses are present; f. reactivity with human subgroups A-E, simian, bovine, canine and swine adenoviruses; and g. lack of reactivity with avian adenovirus.
Claim 6
Dependent Claim
73 words
A monoclonal antibody having the following characteristics: a. being of IgG class of immunoglobulin molecules; b. demonstrating negative neutralization for human adenovirus type 2; c. demonstrating negative precipitation by the Ouchterlony technique for extracts from cells infected with human adenovirus type 2; d. staining nuclei of adenovirus type 2 infected cells positive by indirect immunofluorescence techniques; and e. demonstrating specific reactivity to an antigenic determinant possessed by a plurality of types of adenoviruses.
Claim 7
Dependent Claim
73 words
A monoclonal antibody having the following characteristics: a. being of IgG class of immunoglobulin molecules; b. demonstrating negative neutralization for human adenovirus type 2; c. demonstrating negative precipitation by the Ouchterlony technique for extracts from cells infected with human adenovirus type 2; d. staining nuclei of adenovirus type 2 infected cells positive by indirect immunofluorescence techniques; and e. demonstrating specific reactivity to an antigenic determinant possessed by a plurality of types of adenoviruses.
Claim 8
Dependent Claim
68 words
In process for detecting the presence of adenoviruses comprising contacting sample with antibody and measuring the reaction, the improvement comprises using monoclonal antibodies demonstrating reactivity to an antigenic determinant possessed by a plurality of types of adenoviruses and characterized further by its reactivity with hexon trimers derived from adenoviruses and its lack of reactivity in immunoprecipitation reactions with intact adenovirus virions, denatured hexon polypeptides and monomeric hexon polypeptides.
Claim 9
Dependent Claim
68 words
In process for detecting the presence of adenoviruses comprising contacting sample with antibody and measuring the reaction, the improvement comprises using monoclonal antibodies demonstrating reactivity to an antigenic determinant possessed by a plurality of types of adenoviruses and characterized further by its reactivity with hexon trimers derived from adenoviruses and its lack of reactivity in immunoprecipitation reactions with intact adenovirus virions, denatured hexon polypeptides and monomeric hexon polypeptides.
Claim 10
Dependent Claim
18 words
The process of claim 5 wherein the step of testing the sample further comprises testing by complement fixation.
Claim 11
Dependent Claim
17 words
The process of claim 5 wherein the step of testing the sample further comprises testing by immunofluorescence.
Claim 12
Dependent Claim
17 words
The process of claim 5 wherein the step of testing the sample further comprises testing by radioimmunoassay.
Claim 13
Dependent Claim
18 words
The process of claim 5 wherein the step of testing the sample further comprises testing by Elisa assay.
Claim 14
Dependent Claim
17 words
The process of claim 5 wherein the step of testing the sample further comprises testing by immunoprecipitation.
Claim 15
Dependent Claim
18 words
The process of claim 5 wherein the step of testing the sample further comprises testing by immunoelectron microscopy.
Claim 16
Dependent Claim
55 words
A hybridoma cell line characterized by its production of monoclonal antibodies demonstrating specific reactivity to an antigenic determinant possessed by a plurality of types of adenoviruses, the cell line further characterized by its production of monoclonal antibodies demonstrating a lack of reactivity in immunoprecipitation reactions with intact adenoviruses, denatured hexon polypeptides, and monomeric hexon polypeptides.
Claim 17
Dependent Claim
55 words
A hybridoma cell line characterized by its production of monoclonal antibodies demonstrating specific reactivity to an antigenic determinant possessed by a plurality of types of adenoviruses, the cell line further characterized by its production of monoclonal antibodies demonstrating a lack of reactivity in immunoprecipitation reactions with intact adenoviruses, denatured hexon polypeptides, and monomeric hexon polypeptides.
Claim 18
Dependent Claim
11 words
An antibody-producing cell line having the identifying characteristics of ATCC HB8117.
Claim 19
Dependent Claim
11 words
An antibody-producing cell line having the identifying characteristics of ATCC HB8117.
Claim 20
Independent Claim
80 words
A method of isolation of hybricomas secreting monoclonal antibodies to adenovirus group specific antigenic determinants comprising screening a group of hybridomas for antigen-antibody reactions to hexon trimers, testing the hybridomas, which demonstrate positive reactions with the hexon trimers derived from adenoviruses, for cross reactivity with various adenovirus types lack reactivity in immunoprecipitation reactions with intact adenovirus virions, denatured hexon polypeptides and monomeric hexon polypeptides and separating the hybridomas that demonstrate cross-reactivity in addition to positive reactions with the hexon trimers.
Claim 21
Independent Claim
80 words
A method of isolation of hybricomas secreting monoclonal antibodies to adenovirus group specific antigenic determinants comprising screening a group of hybridomas for antigen-antibody reactions to hexon trimers, testing the hybridomas, which demonstrate positive reactions with the hexon trimers derived from adenoviruses, for cross reactivity with various adenovirus types lack reactivity in immunoprecipitation reactions with intact adenovirus virions, denatured hexon polypeptides and monomeric hexon polypeptides and separating the hybridomas that demonstrate cross-reactivity in addition to positive reactions with the hexon trimers.
Claim 22
Independent Claim
50 words
A method of analyzing chemical compounds for affinity to a group specific determinant of adenoviruses by measuring their competition with monoclonal antibodies which demonstrate specific reactivity with hexon trimers derived from adenoviruses and a lack of reactivity in immunoprecipitation reactions with intact adenoviruses, denatured hexon polypeptides, and monomeric hexon polypeptides.
Claim 23
Independent Claim
50 words
A method of analyzing chemical compounds for affinity to a group specific determinant of adenoviruses by measuring their competition with monoclonal antibodies which demonstrate specific reactivity with hexon trimers derived from adenoviruses and a lack of reactivity in immunoprecipitation reactions with intact adenoviruses, denatured hexon polypeptides, and monomeric hexon polypeptides.
References Cited (Backward Citations)
US4053284A
Cited
US4196265A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-12-11 | 1988-06-11 | 1988-12-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-11 | 1992-06-11 | 1992-12-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-11 | 1996-06-11 | 1996-12-11 | $7700.00 | Window Closed / Lapsed |