Patent US4482636A successfully refreshed from Google Patents.
US4482636A
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Method for reacting solid and liquid phases
Assignee: Mochida Pharmaceutical Co Ltd
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Filing: 1982-08-13
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Granted: 1984-11-13
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Expires: 2002-08-13
Abstract
Abstract A method of reacting a first reactive substance bonded to the inner wall surface of a reaction vessel and a second reactive substance in a liquid phase in the vessel by rotating the reaction vessel about its axis at a speed of 10 to 100 rpm, while said reaction vessel is kept inclined at an angle between 5° and 45° to the horizon with its mouth being raised; and an apparatus usable for practicing this method.
Inventors
- 👤 Ei Mochida
- 👤 Takashi Kudo
- 👤 Toshiyuki Sugawara
- 👤 Minoru Tsumura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 05:09
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; B; B01; B01J; B01J19/00; B01J19/28; B01F; B01F29/00; B01F29/30; B01F29/31; B01J16/00; B01J2219/00; B01J2219/00049; B01J2219/00189; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/811; Y10S436/813
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
75 words
A method of reacting a first reactive substance bound to the inner wall surface of a reaction vessel and a second reactive substance in a liquid phase, said method comprising placing a liquid containing said second reactive substance in said reaction vessel and rotating said reaction vessel about its axis at a speed of 10 to 100 rpm, while said reaction vessel is kept inclined at an angle between 5° and 45° above the horizontal.
Claim 2
Independent Claim
75 words
A method of reacting a first reactive substance bound to the inner wall surface of a reaction vessel and a second reactive substance in a liquid phase, said method comprising placing a liquid containing said second reactive substance in said reaction vessel and rotating said reaction vessel about its axis at a speed of 10 to 100 rpm, while said reaction vessel is kept inclined at an angle between 5° and 45° above the horizontal.
Claim 3
Dependent Claim
25 words
A method as set forth in claim 1, wherein the reaction vessel is kept inclined at an angle between 10° and 20° above the horizontal.
Claim 4
Dependent Claim
21 words
A method as set forth in claim 1 or 2, wherein the speed of rotation is from 25 to 55 rpm.
Claim 5
Dependent Claim
31 words
A method as set forth in claim 1 or 2, wherein said first reactive substance is an antigen or antibody, while said second reactive substance is a corresponding antibody or antigen.
Claim 6
Dependent Claim
55 words
A method as set forth in claim 1 or 2, wherein said first reactive substance is an antibody, while said second reactive substance is a mixture of a corresponding antigen and a labeled antigen obtained by labeling said antigen with a labeling agent selected from the group consisting of radioactive isotopes, enzymes and fluorescent substances.
Claim 7
Dependent Claim
59 words
A method as set forth in claim 1 or 2, wherein said first reactive substance is a complex of an insolubilized antibody and a corresponding antigen, while said second reactive substance is a labeled antibody corresponding to said insolubilized reactive substance and labeled with a labeling agent selected from the group consisting of radioactive isotopes, enzymes and fluorescent substances.
Claim 8
Dependent Claim
36 words
A method as set forth in claim 1 or 2, wherein said first reactive substance is a complex of an insolubilized antibody and a corresponding labeled antigen, while said second reactive substance is an enzyme substrate.
Claim 9
Dependent Claim
41 words
A method as set forth in claim 1 or 2, wherein said first reactive substance is a complex of an insolubilized antibody, a corresponding antigen and an antibody labeled with an enzyme, while said second reactive substance is an enzyme substrate.
Claim 10
Dependent Claim
42 words
A method as set forth in claim 1 or 2, wherein said reaction vessel is a circular bottomed cylinder made of a material selected from the group consisting of glass and plastic, and has an inside diameter of 5 to 20 mm.
Claim 11
Dependent Claim
43 words
A method as set forth in claim 1 or 2, wherein said first reaction vessel is a polygonal bottomed cylinder made of a material selected from the group consisting of glass and plastic, and has an inside diameter of 5 to 20 mm.
Claim 12
Dependent Claim
29 words
A method as set forth in claim 3, wherein said first reactive substance is an antigen or antibody, while said second reactive substance is a corresponding antibody or antigen.
Claim 13
Dependent Claim
53 words
A method as set forth in claim 3, wherein said first reactive substance is an antibody, while said second reactive substance is a mixture of a corresponding antigen and a labeled antigen obtained by labeling said antigen with a labeling agent selected from the group consisting of radioactive isotopes, enzymes and fluorescent substances.
Claim 14
Dependent Claim
57 words
A method as set forth in claim 3, wherein said first reactive substance is a complex of an insolubilized antibody and a corresponding antigen, while said second reactive substance is a labeled antibody corresponding to said insolubilized reactive substance and labeled with a labeling agent selected from the group consisting of radioactive isotopes, enzymes and fluorescent substances.
Claim 15
Dependent Claim
34 words
A method as set forth in claim 3, wherein said first reactive substance is a complex of an insolubilized antibody and a corresponding labeled antigen, while said second reactive substance is an enzyme substrate.
Claim 16
Dependent Claim
39 words
A method as set forth in claim 3, wherein said first reactive substance is a complex of an insolubilized antibody, a corresponding antigen and an antibody labeled with an enzyme, while said second reactive substance is an enzyme substrate.
Claim 17
Dependent Claim
40 words
A method as set forth in claim 3, wherein said reaction vessel is a circular bottomed cylinder made of a material selected from the group consisting of glass and plastic, and has an inside diameter of 5 to 20 mm.
Claim 18
Dependent Claim
40 words
A method as set forth in claim 3, wherein said reaction vessel is a polygonal bottomed cylinder made of a material selected from the group consisting of glass and plastic, and has an inside diameter of 5 to 20 mm.
Claim 19
Independent Claim
68 words
An apparatus for rotating one or more reaction vessels in an inclined position, comprising one or more lateral rows of equally spaced holders projecting from an outer surface of a frame, means for rotating said holders in the same direction at a predetermined speed, and means for positioning said frame in such a manner that said holders may be inclined upwardly at a predetermined angle to the horizontal.
Claim 20
Independent Claim
68 words
An apparatus for rotating one or more reaction vessels in an inclined position, comprising one or more lateral rows of equally spaced holders projecting from an outer surface of a frame, means for rotating said holders in the same direction at a predetermined speed, and means for positioning said frame in such a manner that said holders may be inclined upwardly at a predetermined angle to the horizontal.
Claim 21
Dependent Claim
30 words
An apparatus as set forth in claim 18, wherein said predetermined speed is within a range of 10 to 100 rpm and said predetermined angle is from 5° to 45°.
Claim 22
Dependent Claim
18 words
An apparatus as set forth in claim 19, wherein the predetermined speed is from 25 to 55 rpm.
Claim 23
Dependent Claim
19 words
An apparatus as set forth in claim 19 or 20, wherein the predetermined angle is from 10° to 20°.
Claim 24
Independent Claim
88 words
An apparatus for rotating at least one reaction vessel in an inclined position, comprising a conveyor means having a conveying surface on which freely rotatable rollers are mounted, means for driving said conveyor means in one direction at a speed having a prescribed range, a belt contacting said reaction vessel mounted on said rollers and moving to rotate said reaction vessel in a predetermined direction at a predetermined speed, and means for holding said reaction vessel in an upwardly inclined position at a predetermined angle to the horizontal.
Claim 25
Independent Claim
88 words
An apparatus for rotating at least one reaction vessel in an inclined position, comprising a conveyor means having a conveying surface on which freely rotatable rollers are mounted, means for driving said conveyor means in one direction at a speed having a prescribed range, a belt contacting said reaction vessel mounted on said rollers and moving to rotate said reaction vessel in a predetermined direction at a predetermined speed, and means for holding said reaction vessel in an upwardly inclined position at a predetermined angle to the horizontal.
Claim 26
Dependent Claim
27 words
An apparatus as set forth in claim 22, wherein said predetermined speed is from 10 to 100 rpm and said predetermined angle is from 5° to 45°.
Claim 27
Dependent Claim
18 words
An apparatus as set forth in claim 23, wherein the predetermined speed is from 25 to 55 rpm.
Claim 28
Dependent Claim
19 words
An apparatus as set forth in claim 23 or 24, wherein the predetermined angle is from 10° to 20°.
Claim 29
Dependent Claim
16 words
An apparatus as set forth in claim 22, wherein said conveyor means is a conveyor chain.
Claim 30
Dependent Claim
16 words
An apparatus as set forth in claim 23, wherein said conveyor means is a conveyor chain.
Claim 31
Dependent Claim
16 words
An apparatus as set forth in claim 24, wherein said conveyor means is a conveyor chain.
Claim 32
Dependent Claim
16 words
An apparatus as set forth in claim 25, wherein said conveyor means is a conveyor chain.
References Cited (Backward Citations)
FR814955A
Cited
US2395593A
Cited
US3790663A
Cited
GB1420663A
Cited
US4090848A
Cited
US4297104A
Cited
US4244694A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-11-13 | 1988-05-14 | 1988-11-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-11-13 | 1992-05-14 | 1992-11-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-11-13 | 1996-05-14 | 1996-11-13 | $7700.00 | Window Closed / Lapsed |