Patent US4478939A successfully refreshed from Google Patents.
US4478939A
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SPS, SPS-ase and method for producing SPS-ase
Assignee: Novozymes AS
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Filing: 1981-12-24
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Granted: 1984-10-23
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Expires: 2001-12-24
Abstract
Abstract SPS-A novel pectic-like polysaccharide derived from soy plant cell walls characterized by capability to bind to proteins. SPS-ase-The carbohydrase complex capable of decomposing SPS into decomposition products incapable of attaching to protein, and method for producing SPS-ase by cultivation of an SPS-ase producing microorganism for which preferred microorganism strains are Aspergillus aculeatus CBS 101.43 and Aspergillus japonicus IFO 4408.
Inventors
- 👤 Jens L. Adler-Nissen
- 👤 Henrik Gurtler
- 👤 Georg W. Jensen
- 👤 Hans A. S. Olsen
- 👤 Steen Riisgaard
- 👤 Martin Schulein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 08:17
CPC Classifications
C; C12; C12Y; C12Y302/00; C12Y302/01; C12Y302/01015; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; A; A23; A23J; A23J1/00; A23J1/14; A23J1/148
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
27 words
SPS-ase, a carbohydrase complex capable of decomposing the water soluble polysaccharide component of soy remanence that binds to proteins into decomposition products incapable of attaching to protein.
Claim 2
Dependent Claim
27 words
SPS-ase, a carbohydrase complex capable of decomposing the water soluble polysaccharide component of soy remanence that binds to proteins into decomposition products incapable of attaching to protein.
Claim 3
Dependent Claim
10 words
The SPS-ase of claim 1 produced by Aspergillus aculeatus CBS-101.43.
Claim 4
Dependent Claim
59 words
Method for production of SPS-ase, a carbohydrase complex, capable of decomposing the water soluble polysaccharide component of soy remanence that binds to proteins which comprises cultivating a SPS-ase producing microorganism selected from the group consisting of Aspergillus aculeatus CPS-101.43, Aspergillus japonicus IFO 4408 and mutants thereof in a nutrient medium, followed by recovering the SPS-ase from the cultivation broth.
Claim 5
Dependent Claim
59 words
Method for production of SPS-ase, a carbohydrase complex, capable of decomposing the water soluble polysaccharide component of soy remanence that binds to proteins which comprises cultivating a SPS-ase producing microorganism selected from the group consisting of Aspergillus aculeatus CPS-101.43, Aspergillus japonicus IFO 4408 and mutants thereof in a nutrient medium, followed by recovering the SPS-ase from the cultivation broth.
Claim 6
Dependent Claim
38 words
A method according to claim 3, wherein cultivation is carried out by submerged cultivation at a pH in the range of from 3 to 7, at a temperature in the range of from 20° C. to 40° C.
Claim 7
Dependent Claim
14 words
A method according to claim 3, wherein the nutrient medium contains toasted soy meal.
Claim 8
Dependent Claim
15 words
A method according to claim 5, wherein the soymeal is pre-treated with a proteolytic enzyme.
Claim 9
Dependent Claim
17 words
A method according to claim 3, wherein pectin is added to the nutrient medium during the cultivation.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-23 | 1988-04-23 | 1988-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-23 | 1992-04-23 | 1992-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-23 | 1996-04-23 | 1996-10-23 | $7700.00 | Window Closed / Lapsed |