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US4477437A
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Substances having carcinostatic and immunostimulating activity
Assignee: Toyama Chemical Co Ltd
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Filing: 1982-02-11
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Granted: 1984-10-16
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Expires: 2002-02-11
Abstract
Abstract Novel TF-2 substances having carcinostatic and immunostimulating activity, which are obtained from a culture or its supernatant fluid prepared by culturing bacteria belonging to Fusobacterium genus. Said substances are useful for the treatment of cancerous diseases of the mammals including human being. This disclosure concerns such substances and a process for preparing the same and a carcinostatic agent containing the same.
Inventors
- 👤 Kenzo Tamai
- 👤 Isamu Saikawa
- 👤 Takashi Yasuda
- 👤 Shohachi Murakami
- 👤 Toyoo Maeda
- 👤 Hisatsugu Tsuda
- 👤 Hiroshi Sakai
- 👤 Masatoshi Sugita
- 👤 Yoshiko Yamamoto
- 👤 Hisashi Minami
- 👤 Takako Hori
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 20:42
CPC Classifications
C; C12; C12P; C12P1/00; C12P1/04; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
221 words
A TF-210 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 160° to 235° C.; (e) its infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1510, 1440, 1380, 1240-1220 and 1120-1020 cm -1 ; (f) the ultraviolet absorption spectrum of an aqueous solution of its water-soluble fraction at a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 248-265 nm; (g) it is positive in Molisch reaction, phenolsulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction; (h) has elementary analysis values of C: 40-43% H: 5-7% N: 9-10%; and (i) the saccharide content of the water-soluble fraction at a pH of 7 as determined by a phenolsulfuric acid method is between about 5-25% by weight in terms of glucose, and the protein content determined by Lowry-Folin's method is between about 20-50% by weight in terms of bovine serum albumin.
Claim 2
Dependent Claim
221 words
A TF-210 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 160° to 235° C.; (e) its infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1510, 1440, 1380, 1240-1220 and 1120-1020 cm -1 ; (f) the ultraviolet absorption spectrum of an aqueous solution of its water-soluble fraction at a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 248-265 nm; (g) it is positive in Molisch reaction, phenolsulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction; (h) has elementary analysis values of C: 40-43% H: 5-7% N: 9-10%; and (i) the saccharide content of the water-soluble fraction at a pH of 7 as determined by a phenolsulfuric acid method is between about 5-25% by weight in terms of glucose, and the protein content determined by Lowry-Folin's method is between about 20-50% by weight in terms of bovine serum albumin.
Claim 3
Dependent Claim
223 words
A TF-220 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 160° to 240° C; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1510, 1440, 1380, 1240-1220 and 1120-1020 cm -1 ; (f) the ultraviolet absorption spectrum of an aqueous solution of its water-soluble fraction at a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 248-266 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction; (h) has elementary analysis values of C: 40-42% H: 5-7% N: 7-9%; and (i) the saccharide content of the water-soluble fraction at a pH of 7 as determined by a phenol-sulfuric acid method is between about 5-20% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is about 10% by weight or less in terms of bovine serum albumin.
Claim 4
Dependent Claim
223 words
A TF-220 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 160° to 240° C; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1510, 1440, 1380, 1240-1220 and 1120-1020 cm -1 ; (f) the ultraviolet absorption spectrum of an aqueous solution of its water-soluble fraction at a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 248-266 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction; (h) has elementary analysis values of C: 40-42% H: 5-7% N: 7-9%; and (i) the saccharide content of the water-soluble fraction at a pH of 7 as determined by a phenol-sulfuric acid method is between about 5-20% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is about 10% by weight or less in terms of bovine serum albumin.
Claim 5
Dependent Claim
218 words
A TF-230 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 185° to 225° C; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1510, 1440, 1380, 1240-1220 and 1120-1020 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 249-264 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction; (h) has elementary analysis values of C: 42-45% H: 5-7% N: 10-11%; and (i) the saccharide content of the water-soluble fraction at a pH of 7 as determined by a phenol-sulfuric acid method is between about 5-25% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is between about 30-60% by weight in terms of bovine serum albumin.
Claim 6
Dependent Claim
218 words
A TF-230 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 185° to 225° C; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1510, 1440, 1380, 1240-1220 and 1120-1020 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 249-264 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction; (h) has elementary analysis values of C: 42-45% H: 5-7% N: 10-11%; and (i) the saccharide content of the water-soluble fraction at a pH of 7 as determined by a phenol-sulfuric acid method is between about 5-25% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is between about 30-60% by weight in terms of bovine serum albumin.
Claim 7
Dependent Claim
210 words
A TF-240 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 200° to 215° C.; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1520, 1410-1360, 1280-1210, 1060, 960 and 820 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 250-265 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction; (h) has elementary analysis values of C: 35-38% H: 4-5% N: 12-14%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 15-35% by weight in terms of glucose; and the protein content as determined by Lowry-Folin's method is between about 20-30% by weight in terms of bovine serum albumin.
Claim 8
Dependent Claim
210 words
A TF-240 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 200° to 215° C.; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1520, 1410-1360, 1280-1210, 1060, 960 and 820 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 250-265 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction; (h) has elementary analysis values of C: 35-38% H: 4-5% N: 12-14%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 15-35% by weight in terms of glucose; and the protein content as determined by Lowry-Folin's method is between about 20-30% by weight in terms of bovine serum albumin.
Claim 9
Dependent Claim
160 words
A TF-250 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is soluble in water, but insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 165° to 210° C.; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1510, 1410-1380, 1240-1210, 1150-1120, 1080-1020, 980 and 810 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 248-269 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction;
Claim 10
Dependent Claim
160 words
A TF-250 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is soluble in water, but insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and decomposes at 165° to 210° C.; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3600-3200, 2950-2920, 1680-1620, 1550-1510, 1410-1380, 1240-1210, 1150-1120, 1080-1020, 980 and 810 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 248-269 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction;
Claim 11
Dependent Claim
55 words
(h) has elementary analysis values of C: 30-33% H: 3-5% N: 3-5%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 60-80% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is between about 5-20% by weight in terms of bovine serum albumin.
Claim 12
Dependent Claim
55 words
(h) has elementary analysis values of C: 30-33% H: 3-5% N: 3-5%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 60-80% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is between about 5-20% by weight in terms of bovine serum albumin.
Claim 13
Dependent Claim
236 words
A TF-300 (TF-310, TF-320 and TF-330) product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is soluble in water, but insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and begins to decompose at about 180° C. and remarkably decomposes at not less than 195° C.; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3500-3300, 2920, 2850, 1660-1620, 1580-1540, 1460-1400, 1380-1360, 1120, 1080-1020, 970 and 820-800 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 246-280 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction, but negative in ninhydrin reaction; (h) has elementary analysis values of C: 38-47% H: 5-7% N: 1-4%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 16-60% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is about 10% by weight or less in terms of bovine serum albumin.
Claim 14
Dependent Claim
236 words
A TF-300 (TF-310, TF-320 and TF-330) product having the following properties, or an alkali metal or an alkaline earth metal salt thereof: (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor, Ehrlich solid tumor, Sarcoma 180 and B-16 Melanoma of mouse, and has an immunostimulating activity; (c) it is soluble in water, but insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and begins to decompose at about 180° C. and remarkably decomposes at not less than 195° C.; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3500-3300, 2920, 2850, 1660-1620, 1580-1540, 1460-1400, 1380-1360, 1120, 1080-1020, 970 and 820-800 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 246-280 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction, but negative in ninhydrin reaction; (h) has elementary analysis values of C: 38-47% H: 5-7% N: 1-4%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 16-60% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is about 10% by weight or less in terms of bovine serum albumin.
Claim 15
Dependent Claim
42 words
A TF-300 (TF-310, TF-320, TF-330), product or an alkali metal or an alkaline earth metal salt thereof according to claim 6, wherein the saccharide content as determined by the phenol-sulfuric acid method is between about 16-30% by weight in terms of glucose.
Claim 16
Dependent Claim
42 words
A TF-300 (TF-310, TF-320, TF-330) product or an alkali metal or an alkaline earth metal salt thereof according to claim 6, wherein the saccharide content as determined by the phenol-sulfuric acid method is between about 20-60% by weight in terms of glucose.
Claim 17
Dependent Claim
223 words
A TF-340 product having the following properties, or an alkali metal or an alkali earth metal salt thereof; (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor of mouse, and has an immunostimulating activity; (c) it is soluble in water but insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and begins to decompose at about 140° C. and decomposes remarkably at 200° C. or more; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3500-3300, 2920, 2850, 1660-1640, 1580-1520, 1460-1440, 1410-1340, 1250-1220, 1120-1030, 970 and 835 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 250-265 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction, but negative in ninhydrin reaction; (h) has elementary analysis values of C: 32-34% H: 4-6% N: 3-5%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 20-50% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is about 10% by weight or less in terms of bovine serum albumin.
Claim 18
Dependent Claim
223 words
A TF-340 product having the following properties, or an alkali metal or an alkali earth metal salt thereof; (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor of mouse, and has an immunostimulating activity; (c) it is soluble in water but insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point and begins to decompose at about 140° C. and decomposes remarkably at 200° C. or more; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3500-3300, 2920, 2850, 1660-1640, 1580-1520, 1460-1440, 1410-1340, 1250-1220, 1120-1030, 970 and 835 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 250-265 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction, but negative in ninhydrin reaction; (h) has elementary analysis values of C: 32-34% H: 4-6% N: 3-5%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 20-50% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is about 10% by weight or less in terms of bovine serum albumin.
Claim 19
Dependent Claim
221 words
A TF-350 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof; (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor of mouse, and has an immunostimulating activity; (c) it is soluble in water, but insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point, and begins to decompose at about 110° C. and decomposes remarkably at 180° C. or more; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3500-3300, 2920-2900, 1660-1630, 1580-1520, 1460-1340, 1140-1100, 1080-1020, 970 and 820-800 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 245-264 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction but negative in ninhydrin reaction; (h) has elementary analysis values of C: 34-37% H: 5-6% N: 1-2%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 80-95% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is about 10% by weight or less in terms of bovine serum albumin.
Claim 20
Dependent Claim
221 words
A TF-350 product having the following properties, or an alkali metal or an alkaline earth metal salt thereof; (a) grayish white-light brown powder; (b) it prohibits the proliferation of Ehrlich ascites tumor of mouse, and has an immunostimulating activity; (c) it is soluble in water, but insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether; (d) it has no clear melting point, and begins to decompose at about 110° C. and decomposes remarkably at 180° C. or more; (e) the infrared absorption spectrum obtained by a KBr tablet method has absorption bands at approximately 3500-3300, 2920-2900, 1660-1630, 1580-1520, 1460-1340, 1140-1100, 1080-1020, 970 and 820-800 cm -1 ; (f) the ultraviolet absorption spectrum of its aqueous solution having a pH of 7.0 shows a strong absorption at the absorption edge, and shows an absorption peak at approximately 245-264 nm; (g) it is positive in Molisch reaction, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction and Lowry-Folin's reaction but negative in ninhydrin reaction; (h) has elementary analysis values of C: 34-37% H: 5-6% N: 1-2%; and (i) the saccharide content as determined by a phenol-sulfuric acid method is between about 80-95% by weight in terms of glucose, and the protein content as determined by Lowry-Folin's method is about 10% by weight or less in terms of bovine serum albumin.
Claim 21
Dependent Claim
44 words
A composition comprising a product selected from the group consisting of TF-2 (TF-210, TF-220, TF-230, TF-240, TF-250, TF-300 (TF-310, TF-320, TF-330), TF-340, or TF-350) and an alkali metal or an alkaline earth metal salt thereof according to any one of claims 1 to 10.
Claim 22
Dependent Claim
31 words
The composition according to claim 11 comprising a product selected from the group consisting of TF-300 (TF-310, TF-320, or TF-330) and an alkali metal or an alkaline earth metal salt thereof.
References Cited (Backward Citations)
US4237266A
Cited
US4357323A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-16 | 1988-04-16 | 1988-10-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-16 | 1992-04-16 | 1992-10-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-16 | 1996-04-16 | 1996-10-16 | $7700.00 | Window Closed / Lapsed |