US4474887A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the determination of low density lipoprotein
Assignee: Roche Diagnostics GmbH
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Filing: 1982-08-18
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Granted: 1984-10-02
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Expires: 2002-08-18
Abstract
Abstract The present invention provides a process for the determination of the beta -lipoprotein fraction (LDL) in body fluids, especially in serum or plasma, wherein the LDL fraction is selectively precipitated from the sample by the addition of a polyvinyl sulphate with monovalent cations and then determined. The present invention also provides a reagent for the determination of the beta -lipoprotein fraction (LDL) in body fluids, especially in serum or plasma, wherein it contains polyvinyl sulphate.
Inventors
- 👤 Josef Maier
- 👤 Manfred Gloger
- 👤 Brigitte Drager
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 15:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92; G01N2800/00; G01N2800/04; G01N2800/044; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/104165
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
47 words
Method for the determination of the β-lipoprotein fraction (LDL) in body fluids which comprises selectively precipitating the LDL fraction from the body fluid sample by the addition of a polyvinyl sulfate with monovalent cations in the substantial absence of divalent cations, and then determining this LDL fraction.
Claim 2
Dependent Claim
47 words
Method for the determination of the β-lipoprotein fraction (LDL) in body fluids which comprises selectively precipitating the LDL fraction from the body fluid sample by the addition of a polyvinyl sulfate with monovalent cations in the substantial absence of divalent cations, and then determining this LDL fraction.
Claim 3
Dependent Claim
12 words
Method as claimed in claim 1, wherein the body fluid is serum.
Claim 4
Dependent Claim
12 words
Method as claimed in claim 1, wherein the body fluid is plasma.
Claim 5
Dependent Claim
17 words
Method as claimed in claim 1, wherein a polyglycol methyl ether is additionally added to the sample.
Claim 6
Dependent Claim
15 words
Method as claimed in claim 1, wherein a polyvinylpyrrolidone is additionally added to the sample.
Claim 7
Dependent Claim
29 words
Method as claimed in claim 1, wherein said polyvinyl sulfate is a water-soluble polyvinyl sulfate at least 70% of the vinyl alcohol units of which carry a sulfate group.
Claim 8
Dependent Claim
19 words
Method as claimed in claim 1, wherein 0.1 to 0.01% wt./vol. of polyvinyl sulfate is added to said sample.
Claim 9
Dependent Claim
25 words
Method as claimed in claim 4, wherein said polyglycol methyl ether is added in an amount of 5 to 10% by volume of the sample.
Claim 10
Dependent Claim
23 words
Method as claimed in claim 5, wherein said polyvinylpyrrolidone is added in an amount of 5 to 10% by volume of the sample.
Claim 11
Dependent Claim
29 words
Method as claimed in claim 1, wherein said body fluid sample contains polyvalent cations, further comprising adding a complex former for polyvalent cations to reduce the divalent cation content.
Claim 12
Dependent Claim
15 words
Method as claimed in claim 10, wherein a polyaminoacetate is used as the complex former.
Claim 13
Dependent Claim
28 words
Method as claimed in claim 1, wherein the amount of LDL is determined by measuring the cholesterol content in either the precipitate or in the serum and supernatant.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-02 | 1988-04-02 | 1988-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-02 | 1992-04-02 | 1992-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-02 | 1996-04-02 | 1996-10-02 | $7700.00 | Window Closed / Lapsed |