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US4474882A
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Microbiological process for the preparation of unsaturated dicarboxylic acids
Assignee: Daicel Corp
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Filing: 1981-09-21
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Granted: 1984-10-02
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Expires: 2001-09-21
Abstract
Abstract A process for the preparation of an unsaturated dicarboxylic acid which comprises cultivating under aerobic conditions a yeast belonging to Candida tropicalis which is capable of producing an unsaturated dicarboxylic acid from an unsaturated fatty acid or its ester, such as Candida tropicalis 104-04 strain, in a medium containing an unsaturated fatty acid having 14 to 22 carbon atoms or its ester, to produce an unsaturated dicarboxylic acid having 14 to 22 carbon atoms; or effecting oxidation of said fatty acid or its ester in the presence of microorganisms of said yeast produced in advance by cultivation in an assimilable carbon source to produce an unsaturated dicarboxylic acid having 14 to 22 carbon atoms; and then recovering the thus-produced unsaturated dicarboxylic acid.
Inventors
- 👤 Etsumi Kunishige
- 👤 Tsuyoshi Morinaga
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:34
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/44; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/921; Y10S435/924
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
126 words
A process for producing a long-chain, unsaturated, dicarboxylic acid, which comprises: culturing, under aerobic conditions, the strain Candida tropicalis FERM BP-49 capable of converting an unsaturated monocarboxylic acid having (1) 14 to 22 carbon atoms, (2) a carboxyl group at one terminal thereof and (3) a methyl group at the other terminal thereof, or ester thereof, to the corresponding unsaturated dicarboxylic acid having (1) carboxyl groups at both terminals thereof, (2) the same number of total carbon atoms, (3) the same number and position of unsaturated carbon atoms, and (4) the same stereostructure as said unsaturated monocarboxylic acid, in a nutrient medium containing said unsaturated monocarboxylic acid or ester thereof, until said corresponding unsaturated dicarboxylic acid is produced; and then recovering said corresponding unsaturated dicarboxylic acid.
Claim 2
Dependent Claim
126 words
A process for producing a long-chain, unsaturated, dicarboxylic acid, which comprises: culturing, under aerobic conditions, the strain Candida tropicalis FERM BP-49 capable of converting an unsaturated monocarboxylic acid having (1) 14 to 22 carbon atoms, (2) a carboxyl group at one terminal thereof and (3) a methyl group at the other terminal thereof, or ester thereof, to the corresponding unsaturated dicarboxylic acid having (1) carboxyl groups at both terminals thereof, (2) the same number of total carbon atoms, (3) the same number and position of unsaturated carbon atoms, and (4) the same stereostructure as said unsaturated monocarboxylic acid, in a nutrient medium containing said unsaturated monocarboxylic acid or ester thereof, until said corresponding unsaturated dicarboxylic acid is produced; and then recovering said corresponding unsaturated dicarboxylic acid.
Claim 3
Dependent Claim
57 words
A process as claimed in claim 1, in which said unsaturated monocarboxylic acid is myristoleic acid, 2-hexadecenoic acid, palmitoleic acid, oleic acid, elaidic acid, linoleic acid, linolenic acid or erucic acid, and the corresponding unsaturated dicarboxylic acid produced is 5-tetradecenedioic acid, 2-hexadecenedioic acid, 7-hexadecenedioic acid, 9-cis-octadecenedioic acid, 9-trans-octadecenedioic acid, 6,9-octadecadienedioic acid, 3,6,9-octadecatrienedioic acid or 5-docosenedioic acid, respectively.
Claim 4
Dependent Claim
151 words
A process for producing a long-chain, unsaturated, dicarboxylic acid, which comprises: growing, in a nutrient medium, the strain Candida tropicalis FERM BP-49 capable of converting an unsaturated monocarboxylic acid having (1) 14 to 22 carbon atoms, (2) a carboxyl group at one terminal thereof, and (3) a methyl group at the other terminal thereof, or ester thereof, to the corresponding unsaturated dicarboxylic acid having (1) carboxyl groups at both terminals thereof, (2) the same number of total carbon atoms, (3) the same number and position of unsaturated carbon atoms, and (4) the same stereostructure as said unsaturated monocarboxylic acid; separating the cell mass comprising said strain from the nutrient medium; and contacting said cell mass, under aerobic conditions, with said unsaturated monocarboxylic acid, or ester thereof, to effect enzymatic oxidation of said unsaturated monocarboxylic acid until said corresponding unsaturated dicarboxylic acid is produced; and then recovering said corresponding unsaturated dicarboxylic acid.
Claim 5
Dependent Claim
151 words
A process for producing a long-chain, unsaturated, dicarboxylic acid, which comprises: growing, in a nutrient medium, the strain Candida tropicalis FERM BP-49 capable of converting an unsaturated monocarboxylic acid having (1) 14 to 22 carbon atoms, (2) a carboxyl group at one terminal thereof, and (3) a methyl group at the other terminal thereof, or ester thereof, to the corresponding unsaturated dicarboxylic acid having (1) carboxyl groups at both terminals thereof, (2) the same number of total carbon atoms, (3) the same number and position of unsaturated carbon atoms, and (4) the same stereostructure as said unsaturated monocarboxylic acid; separating the cell mass comprising said strain from the nutrient medium; and contacting said cell mass, under aerobic conditions, with said unsaturated monocarboxylic acid, or ester thereof, to effect enzymatic oxidation of said unsaturated monocarboxylic acid until said corresponding unsaturated dicarboxylic acid is produced; and then recovering said corresponding unsaturated dicarboxylic acid.
Claim 6
Dependent Claim
57 words
A process as claimed in claim 3, in which said unsaturated monocarboxylic acid is myristoleic acid, 2-hexadecenoic acid, palmitoleic acid, oleic acid, elaidic acid, linoleic acid, linolenic acid or erucic acid, and the corresponding unsaturated dicarboxylic acid produced is 5-tetradecenedioic acid, 2-hexadecenedioic acid, 7-hexadecenedioic acid, 9-cis-octadecenedioic acid, 9-trans-octadecenedioic acid, 6,9-octadecadienedioic acid, 3,6,9-octadecatrienedioic acid or 5-docosenedioic acid, respectively.
Claim 7
Dependent Claim
53 words
A process as claimed in claim 1 or claim 3 in which said unsaturated monocarboxylic acid has the formula CH.sub.3 --R--COOH wherein R is a divalent aliphatic hydrocarbon group containing from 12 to 20 carbon atoms and having from 1 to 4 double bonds, and said unsaturated dicarboxylic acid has the formula HOOC--R--COOH.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-02 | 1988-04-02 | 1988-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-02 | 1992-04-02 | 1992-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-02 | 1996-04-02 | 1996-10-02 | $7700.00 | Window Closed / Lapsed |