Patent US4470967A successfully refreshed from Google Patents.
US4470967A
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Lectin-containing anti-viral vaccines for domestic animals and method of preparation
Assignee: Iowa State University Research Foundation Inc
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Filing: 1982-10-05
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Granted: 1984-09-11
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Expires: 2002-10-05
Abstract
Abstract Anti-viral vaccines for domestic animals are prepared from glycoprotein envelope whole virus or antigenic glycoprotein obtained therefrom by complexing the glycoprotein antigenic agent with a lectin, which is preferably a mitogenic lectin. The recovery of the immunizing agent from an aqueous solution or suspension is facilitated since the lectin-glycoprotein complex is readily recoverable. The mitogenic lectin administered in the vaccine with the glycoprotein antigen can enhance the anamnestic response, in effect, acting as an adjuvant.
Inventors
- 👤 Patricia M. Gough
- 👤 Kenneth B. Platt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 13:32
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/39; A61K39/12; A61K39/215; A61K39/225; A61K39/245; A61K2039/51; A61K2039/525; A61K2039/545; A61K2039/55; A61K2039/552; A61K2039/555; A61K2039/55511; A61K2039/55516; C; C12; C12N; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16711; C12N2710/16734; C12N2770/00; C12N2770/00011; C12N2770/20011; C12N2770/20034
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
88 words
A veterinary vaccine in parenterally injectable dose form, comprising an effective immunizing dose amount of viral antigen immunizing agent selected from the class consisting of (i) whole viruses having envelopes containing at least one glycoprotein or (ii) the separated viral envelope glycoprotein obtained from such viruses, and a mitogenic lectin complexed with the glycoprotein of said immunizing agent, said complexed lectin providing binding sites available for lymphocytes, and said vaccine containing at least from 0.01 to 0.1 milligrams (mg) of said mitogenic lectin per milligram (mg) of glycoprotein.
Claim 2
Dependent Claim
88 words
A veterinary vaccine in parenterally injectable dose form, comprising an effective immunizing dose amount of viral antigen immunizing agent selected from the class consisting of (i) whole viruses having envelopes containing at least one glycoprotein or (ii) the separated viral envelope glycoprotein obtained from such viruses, and a mitogenic lectin complexed with the glycoprotein of said immunizing agent, said complexed lectin providing binding sites available for lymphocytes, and said vaccine containing at least from 0.01 to 0.1 milligrams (mg) of said mitogenic lectin per milligram (mg) of glycoprotein.
Claim 3
Dependent Claim
20 words
The vaccine of claim 1 in which said immunizing agent comprises transmissible gastroenteritis (TGE) virus or separated glycoprotein obtained therefrom.
Claim 4
Dependent Claim
15 words
The vaccine of claim 2 in which said immunizing agent comprises separated TGE envelope glycoprotein.
Claim 5
Dependent Claim
18 words
The vaccine of claim 1 in which said immunizing agent comprises pseudorabies (PR) virus or glycoprotein obtained therefrom.
Claim 6
Dependent Claim
16 words
The vaccine of claim 4 in which said immunizing agent comprises separated pseudorabies °PR1 envelope glycoprotein.
Claim 7
Dependent Claim
15 words
The vaccines of claims 1 to 5 in which said lectin is lentil bean lectin.
Claim 8
Independent Claim
163 words
The method of preparing and administering a veterinary vaccine, comprising: (a) preparing an aqueous solution or suspension of a viral antigen immunizing agent selected from the class consisting of (i) whole viruses having envelopes containing at least one glycoprotein or (ii) the separated viral envelope glycoprotein obtained from such viruses; (b) contacting said immunizing agent in said aqueous solution or suspension with a lectin reagent capable of complexing with the glycoprotein of said immunizing agent, said lectin reagent being a mitogenic lectin and a sufficient amount of said lectin reagent being employed to form a readily separable complex from said immunizing agent; (c) recovering the thus-formed lectin-glycoprotein complex of the immunizing agent; (d) preparing a parenterally injectable vaccine from said immunizing agent without separating the lectin therefrom, the lectin in said complex having binding sites available for lymphocytes; and (e) administering an effective immunizing dose amount of said vaccine by parenteral injection to an animal capable of being immunized by said immunizing agent.
Claim 9
Independent Claim
163 words
The method of preparing and administering a veterinary vaccine, comprising: (a) preparing an aqueous solution or suspension of a viral antigen immunizing agent selected from the class consisting of (i) whole viruses having envelopes containing at least one glycoprotein or (ii) the separated viral envelope glycoprotein obtained from such viruses; (b) contacting said immunizing agent in said aqueous solution or suspension with a lectin reagent capable of complexing with the glycoprotein of said immunizing agent, said lectin reagent being a mitogenic lectin and a sufficient amount of said lectin reagent being employed to form a readily separable complex from said immunizing agent; (c) recovering the thus-formed lectin-glycoprotein complex of the immunizing agent; (d) preparing a parenterally injectable vaccine from said immunizing agent without separating the lectin therefrom, the lectin in said complex having binding sites available for lymphocytes; and (e) administering an effective immunizing dose amount of said vaccine by parenteral injection to an animal capable of being immunized by said immunizing agent.
Claim 10
Dependent Claim
19 words
The method of claim 7 in which said lectin is in water-soluble form when complexed with said immunizing agent.
Claim 11
Dependent Claim
22 words
The method of claim 7 in which said lectin when complexed with said immunizing agent is immobilized on a particulate support material.
Claim 12
Independent Claim
16 words
The method of claims 7, 8, or 9 in which said lectin is lentil bean lectin.
Claim 13
Independent Claim
22 words
The method of claims 7, 8, or 9 in which said immunizing agent comprises transmissible gastroenteritis virus or separated glycoprotein obtained therefrom.
Claim 14
Independent Claim
22 words
The method of claims 7, 8, or 9 in which said immunizing agent comprises pseudorabies (PR) virus or separated glycoprotein obtained therefrom.
Claim 15
Dependent Claim
32 words
The method of claim 7 in which said immunizing agent is separated viral envelope glycoprotein, and both said glycoprotein and said lectin are dissolved in said aqueous solution for said complexing reaction.
Claim 16
Dependent Claim
23 words
The method of claim 13 in which said glycoprotein is obtained from transmissible gastroenteritis (TGE) virus, and said lectin is lentil bean lectin.
Claim 17
Dependent Claim
22 words
The method of claim 13 in which said glycoprotein is obtained from pseudorabies (PR) virus, and said lectin is lentil bean lectin.
Claim 18
Dependent Claim
75 words
A veterinary vaccine in parenterally injectable dose form, comprising an effective immunizing dose amount of viral antigen immunizing agent consisting of viral envelope glycoprotein separated from a virus having an envelope containing at least one glycoprotein, and a mitogenic lectin complexed with said immunizing agent, said complexed lectin providing binding sites available for lymphocytes, and said vaccine containing at least from 0.01 to 0.1 milligrams (mg) of said mitogenic lectin per milligram (mg) of glycoprotein.
Claim 19
Dependent Claim
75 words
A veterinary vaccine in parenterally injectable dose form, comprising an effective immunizing dose amount of viral antigen immunizing agent consisting of viral envelope glycoprotein separated from a virus having an envelope containing at least one glycoprotein, and a mitogenic lectin complexed with said immunizing agent, said complexed lectin providing binding sites available for lymphocytes, and said vaccine containing at least from 0.01 to 0.1 milligrams (mg) of said mitogenic lectin per milligram (mg) of glycoprotein.
Claim 20
Independent Claim
146 words
The method of preparing and administering a veterinary vaccine comprising: (a) preparing an aqueous solution of a viral antigen immunizing agent consisting of separated viral envelope glycoprotein obtained from a virus having an envelope containing at least one glycoprotein; (b) contacting said immunizing agent in said aqueous solution with a lectin reagent capable of complexing with the glycoprotein of said immunizing agent, said lectin reagent being a mitogenic lectin and a sufficient amount of said lectin reagent being employed to form a readily separable complex from said immunizing agent; (c) recovering the thus-formed lectin-glycoprotein complex; (d) preparing a parenterally injectable vaccine from said immunizing agent without separating the lectin therefrom the lectin in said complex having binding sites available for lymphocytes; and (e) administering an effective immunizing dose amount of said vaccine by parenteral injecting to an animal capable of being immunized by said immunizing agent.
Claim 21
Independent Claim
146 words
The method of preparing and administering a veterinary vaccine comprising: (a) preparing an aqueous solution of a viral antigen immunizing agent consisting of separated viral envelope glycoprotein obtained from a virus having an envelope containing at least one glycoprotein; (b) contacting said immunizing agent in said aqueous solution with a lectin reagent capable of complexing with the glycoprotein of said immunizing agent, said lectin reagent being a mitogenic lectin and a sufficient amount of said lectin reagent being employed to form a readily separable complex from said immunizing agent; (c) recovering the thus-formed lectin-glycoprotein complex; (d) preparing a parenterally injectable vaccine from said immunizing agent without separating the lectin therefrom the lectin in said complex having binding sites available for lymphocytes; and (e) administering an effective immunizing dose amount of said vaccine by parenteral injecting to an animal capable of being immunized by said immunizing agent.
References Cited (Backward Citations)
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| 3.5 Years (Year 4) | 1987-09-11 | 1988-03-12 | 1988-09-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-11 | 1992-03-12 | 1992-09-11 | $3760.00 | Window Closed / Lapsed |
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