Patent US4460525A successfully refreshed from Google Patents.
US4460525A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for preparing thin layers
Assignee: European Molecular Biology Laboratory
•
Filing: 1981-06-22
•
Granted: 1984-07-17
•
Expires: 2001-06-22
Abstract
Abstract A method and apparatus for preparing thin layers, such as thin gel layers useful in electrophoresis, wherein a liquid layer material is introduced to the space defined between two parallel mold surfaces on first and second mold elements. Initially, the mold surfaces overlap each other to a small extent, and liquid is introduced to the space between the surfaces adjacent the leading edge of the top mold element. The mold elements are then shifted with respect to each other while maintaining their parallel relationship, and additional liquid layer-forming material is introduced to the space during the shift. In a preferred embodiment, a third mold element is situated adjacent to and spaced a small distance from the bottom mold element, and liquid material is introduced to the space between the bottom mold elements during shifting of the top mold.
Inventors
- 👤 Wilhelm Ansorge
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 12:03
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
181 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (c) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (d) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (e) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneously with the shifting of said elements.
Claim 2
Independent Claim
181 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (c) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (d) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (e) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneously with the shifting of said elements.
Claim 3
Dependent Claim
35 words
The method of claim 1 wherein said first element is disposed above said second element with said first surface facing downward, and said liquid material is applied to said surface forwardly of said leading edge.
Claim 4
Dependent Claim
13 words
The method of claim 2 wherein said liquid is applied by dropwise addition.
Claim 5
Dependent Claim
36 words
The method of claim 1 wherein in the event of formation of a bubble between said surfaces, the direction of shift of said elements is reversed until said bubble is exposed forwardly of said leading edge.
Claim 6
Dependent Claim
24 words
The method of claim 1 wherein at least two transversely spaced seals extend between said surfaces in the direction of shift of said elements.
Claim 7
Dependent Claim
45 words
The method of claim 5 wherein each said element includes two opposed spaced sides, and each said seal comprises a strip of low friction material having a thickness substantially equal to the spacing between said surfaces and is disposed adjacent opposed sides of said elements.
Claim 8
Dependent Claim
12 words
The method of claim 6 wherein said low friction material comprises polytetrafluoroethylene.
Claim 9
Dependent Claim
57 words
The method of claim 1 wherein said second mold element includes a trailing edge parallel to the leading edge of said first element, and which underlies said first element when said elements are in the first position, and the space between said surfaces is sealed along said second element trailing edge during the shifting of said elements.
Claim 10
Dependent Claim
25 words
The method of claim 8 wherein said seal along said trailing edge comprises a sealing strip applied to said second element along said trailing edge.
Claim 11
Dependent Claim
51 words
The method of claim 1 wherein said second element includes a trailing edge underlying said first element when the elements are in their first position and additional liquid material is introduced to the space defined between said surfaces at a point adjacent said trailing edge during the shifting of said elements.
Claim 12
Dependent Claim
35 words
The method of claim 1 wherein a third mold element is disposed rearwardly of and adjacent to said second element, said third element having a surface which lies in the plane of said second surface.
Claim 13
Dependent Claim
19 words
The method of claim 11 wherein said second and third elements are spaced a short distance from each other.
Claim 14
Independent Claim
196 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) pretreating one of said surfaces to render said surface repellant to said liquid material; (c) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (d) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (e) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (f) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneously with the shifting of said elements.
Claim 15
Independent Claim
196 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) pretreating one of said surfaces to render said surface repellant to said liquid material; (c) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (d) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (e) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (f) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneously with the shifting of said elements.
Claim 16
Dependent Claim
14 words
The method of claim 13 wherein said surface is rendered hydrophobic by said pretreatment.
Claim 17
Dependent Claim
18 words
The method of claim 13 wherein said pretreatment step comprises providing said mold surface with a gel-repellent coating.
Claim 18
Independent Claim
202 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) pretreating at least one of said surfaces such that the adhesion of said surface to said liquid material is improved; (c) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (d) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (e) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (f) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneously with the shifting of said elements.
Claim 19
Independent Claim
202 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) pretreating at least one of said surfaces such that the adhesion of said surface to said liquid material is improved; (c) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (d) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (e) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (f) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneously with the shifting of said elements.
Claim 20
Dependent Claim
13 words
The method of claim 16 wherein said surface is treated with a silane.
Claim 21
Dependent Claim
11 words
The method of claim 17 wherein said silane comprises γ-methacryloxy-propyltrimethoxy silane.
Claim 22
Independent Claim
234 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (c) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (d) introducing a multiple sample introduction device to the space between said surfaces adjacent said first element leading edge, said device comprising a comb-shaped structure having a backbone with a plurality of teeth extending therefrom with spaces between said teeth, said teeth being captured between said elements adjacent said first element leading edge; (e) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (f) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneously with the shifting of said elements.
Claim 23
Independent Claim
234 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (c) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (d) introducing a multiple sample introduction device to the space between said surfaces adjacent said first element leading edge, said device comprising a comb-shaped structure having a backbone with a plurality of teeth extending therefrom with spaces between said teeth, said teeth being captured between said elements adjacent said first element leading edge; (e) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (f) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneously with the shifting of said elements.
Claim 24
Dependent Claim
19 words
The method of claim 19 wherein said multiple sample introduction device is introduced before the shifting of said elements.
Claim 25
Dependent Claim
19 words
The method of claim 19 wherein said multiple sample introduction device is introduced after the shifting of said elements.
Claim 26
Dependent Claim
19 words
The method of claim 1 wherein said mold elements are pressed together after shifting thereof with a clamping device.
Claim 27
Dependent Claim
16 words
The method of claim 1 wherein said liquid is polymerized after shifting of said mold elements.
Claim 28
Dependent Claim
19 words
The method of claim 23 wherein said polymerization is carried out with said mold elements disposed vertically or horizontally.
Claim 29
Independent Claim
199 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements formed of a material selected from the group consisting of plastic fabrics, cellophane, polystyrene, polyester and glass and having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (c) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (d) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (e) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneouly with the shifting of said elements.
Claim 30
Independent Claim
199 words
A method of preparing a thin layer, said method comprising the steps of: (a) providing first and second mold elements formed of a material selected from the group consisting of plastic fabrics, cellophane, polystyrene, polyester and glass and having first and second mold surfaces, respectively, said first mold element having a straight leading edge; (b) disposing said mold elements in a first position wherein said first and second surfaces are parallel and face each other, and are spaced a selected small distance from each other, with said surfaces overlapping each other with said first element's leading edge overlying said second mold surface; (c) introducing a liquid layer-forming material to the space defined between said first and second mold surfaces adjacent said leading edge; (d) shifting said mold elements relative to each other in a forward direction which is generally perpendicular to the direction of said leading edge from said first position to a second position wherein said mold surfaces are opposed, maintaining said surfaces in parallel relation during the shifting of said element; and (e) introducing said liquid to the space defined between said surfaces at a point adjacent said leading edge simultaneouly with the shifting of said elements.
Claim 31
Dependent Claim
14 words
The method of claim 25 wherein said plastic fabric comprises a polyamide fiber fabric.
Claim 32
Dependent Claim
10 words
The method of claim 26 wherein said fabric comprises nylon.
Claim 33
Dependent Claim
12 words
The method of claim 25 wherein said material comprises polyester or glass.
Claim 34
Dependent Claim
18 words
The method of claim 1 wherein at least one of said mold elements comprises a thermostatically heatable plate.
Claim 35
Dependent Claim
17 words
The method of claim 1 wherein at least one of said molded elements comprises a glass plate.
Claim 36
Dependent Claim
22 words
The method of claim 29 or 30 wherein the mold surface of said heatable plate or glass plate comprises a polyester film.
References Cited (Backward Citations)
US4246222A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-17 | 1988-01-16 | 1988-07-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-17 | 1992-01-16 | 1992-07-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-17 | 1996-01-16 | 1996-07-17 | $7700.00 | Window Closed / Lapsed |