US4458015A
Expired / Lapsed
US
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Reagent for the optical determination of the blood coagulation
Assignee: Roche Diagnostics GmbH
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Filing: 1982-03-19
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Granted: 1984-07-03
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Expires: 2002-03-19
Abstract
Abstract The present invention provides a reagent for the optical determination of blood coagulation behavior (Quick test), comprising thromboplastin, a synthetic thrombin substrate and a buffer, characterized by a content of non-proteolytically active thromboplastin.
Inventors
- 👤 Helmut Jering
- 👤 Udo Becker
- 👤 Peter Roeschlau
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 13:56
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/56; C12Q2337/00; C12Q2337/10; C12Q2337/12; G; G01; G01N; G01N2333/00; G01N2333/435; G01N2333/745; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/974; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
80 words
Reagent for the optical determination of blood coagulation behavior (Quick test), comprising thromboplastin, a synthetic thrombin substrate and a buffer in the range of pH 7.2 to 8.5, said thromboplastin being non-proteolytically-active so as to develop only about 22.7 to 35.7% of the extinction at 405 nm as compared with normal thromboplastin when each is incubated for a period of one to seven days at about 20° C. with tris-HCl buffer of pH 8.1 in the presence of chromogeneous substrate.
Claim 2
Dependent Claim
80 words
Reagent for the optical determination of blood coagulation behavior (Quick test), comprising thromboplastin, a synthetic thrombin substrate and a buffer in the range of pH 7.2 to 8.5, said thromboplastin being non-proteolytically-active so as to develop only about 22.7 to 35.7% of the extinction at 405 nm as compared with normal thromboplastin when each is incubated for a period of one to seven days at about 20° C. with tris-HCl buffer of pH 8.1 in the presence of chromogeneous substrate.
Claim 3
Dependent Claim
67 words
Reagent as claimed in claim 1, wherein the non-proteolytically active thromboplastin is obtained by preparing an acetone dry powder from brain tissue, incubating a neutral aqueous solution of the acetone dry powder at 25° to 40° C., obtaining the insoluble fraction, treating a suspension of this fraction in a dilute salt solution with a surface-active agent in the presence of formate and drying the separate soluble fraction.
Claim 4
Dependent Claim
48 words
Reagent as claimed in claim 1, comprising the non-proteolytic thromboplastin in a concentration of 3 to 20 vol. %, 2×10 -5 to 20×10 -5 mol/liter of synthetic thrombin substrate, 0.05 to 0.25 mol/liter of buffer (pH 7.2 to 8.5) and 2 to 10 mmol/liter of soluble calcium salt.
Claim 5
Dependent Claim
12 words
Reagent as claimed in claim 3, additionally containing 0.5 to 3% urea.
Claim 6
Dependent Claim
13 words
Reagent as claimed in claim 3, wherein the buffer is a tris buffer.
Claim 7
Dependent Claim
46 words
Method for the optical determination of blood coagulation behavior (Quick test) which method comprises contacting a reagent as claimed in claim 1 with a blood sample and measuring the optical characteristics of the resulting mixture as a measure of the blood coagulation behavior of said sample.
Claim 8
Dependent Claim
23 words
Method as claimed in claim 6, wherein the time up to reaching a particular absorption value at a given wave length is measured.
Claim 9
Dependent Claim
19 words
Method as claimed in claim 6, wherein a kinetic evaluation is carried out by means of a calibration curve.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-03 | 1988-01-02 | 1988-07-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-03 | 1992-01-02 | 1992-07-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-03 | 1996-01-02 | 1996-07-03 | $7700.00 | Window Closed / Lapsed |