US4169015A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Novel chromogenic thrombin substrates
Assignee: Chromogenix AB
•
Filing: 1977-08-22
•
Granted: 1979-09-25
•
Expires: 1997-08-22
Abstract
Abstract Process for determining thrombin and thrombin-like enzymes employing a substrate having the formula: <IMAGE> and salts thereof, wherein R1 is H or OH; R2 is a chromophoric group; the N-terminal amino acid is in the D configuration and n is 1, 2 or 3 provided along with the method of use.
Inventors
- 👤 Bo T. Af Ekenstam
- 👤 Leif E. Aurell
- 👤 Karl G. Claeson
- 👤 Birgitta G. Karlsson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 15:17
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C07; C07K; C07K1/00; C07K1/06; C07K1/061; C07K1/064; C12Q1/56; C12Q2337/00; C12Q2337/10; C12Q2337/12; C12Q2337/30; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/974
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
83 words
A method for the quantitative determination of thrombin and thrombin-like enzymes which comprises contacting the substance for which said determination is desired with a diagnostically active chromogenic substrate with a high specificity to thrombin and thrombin-like enzymes having the general formula: ##STR3## and salts thereof, wherein R 1 is hydrogen or hydroxy, R 2 is a chromophoric group, n is 1, 2 or 3, the N terminal amino acid being in the D configuration and then spectrophotometrically measuring the degree of enzymatic hydrolysis.
Claim 2
Independent Claim
83 words
A method for the quantitative determination of thrombin and thrombin-like enzymes which comprises contacting the substance for which said determination is desired with a diagnostically active chromogenic substrate with a high specificity to thrombin and thrombin-like enzymes having the general formula: ##STR3## and salts thereof, wherein R 1 is hydrogen or hydroxy, R 2 is a chromophoric group, n is 1, 2 or 3, the N terminal amino acid being in the D configuration and then spectrophotometrically measuring the degree of enzymatic hydrolysis.
Claim 3
Dependent Claim
10 words
The method of claim 1 wherein R 1 is hydrogen.
Claim 4
Dependent Claim
10 words
The method of claim 1 wherein R 1 is hydroxy.
Claim 5
Dependent Claim
9 words
The method of claim 1 wherein n is 1.
Claim 6
Dependent Claim
9 words
The method of claim 1 wherein n is 2.
Claim 7
Dependent Claim
9 words
The method of claim 1 wherein n is 3.
Claim 8
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H--D--Phe--Pro--Arg--p--NA or salt thereof.
Claim 9
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H--D--Phe--Pip--Arg--pNA or salt thereof.
Claim 10
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H--D--Phe--Aze--Arg--pNA or salt thereof.
Claim 11
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H--D--Tyr--Pip--Arg--pNA or salt thereof.
Claim 12
Dependent Claim
10 words
The method of claim 1 wherein R 2 is nitrophenyl.
Claim 13
Dependent Claim
21 words
The method of claim 1 wherein R 2 is selected from the group of nitrophenyl, naphthyl, nitronaphthyl, methoxynaphthyl, quinolyl, and nitroquinolyl.
Claim 14
Dependent Claim
14 words
The method of claim 1 wherein R 2 is nitrophenyl or naphthyl or methoxynaphthyl.
Claim 15
Dependent Claim
14 words
The method of claim 11 wherein said measuring is carried out at 405 nm.
References Cited (Backward Citations)
US3388113A
Cited
US3867364A
Cited
US3778426A
Cited
US3862011A
Cited
US3884896A
Cited
US3886136A
Cited
US4016042A
Cited
US4070245A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-09-24 | 1983-03-26 | 1983-09-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-09-24 | 1987-03-26 | 1987-09-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-09-24 | 1991-03-26 | 1991-09-25 | $7700.00 | Window Closed / Lapsed |