Abstract A concept and various means of immunological assay are disclosed wherein two different classes of particles which interact at short distances to produce characteristic detectable signals are employed in a modification of the usual latex fixation test. In one embodiment of the concept an aqueous suspension of appropriately coated tritiated latex particles (LH) and polystyrene scintillant particles (L*) is employed. The amount of (LH) (L*) dimer formation and higher order aggregation induced and therefore the concentration of antibody (or antigen) present which caused the aggregation can be determined by using standard liquid scintillation counting equipment. Also, an optical container for use in the assay which has one side provided with a section forming a quadrilateral prism with the prism providing for an angle of incidence greater than the critical angle for an electromagnetic beam of radiation.
A transparent optical container system for use in the non-destructive biological fluid assay of immunological attractive material by excitement of scintillating particulates and the measurement of said excitement, the improvement wherein: said transparent container having a side provided with a section forming a quadrilateral prism, said prism having an interface base provided with immunological linking material for attachment to said scintillating particulates, said prism inherently providing an angle of incidence greater than the critical angle for an electromagnetic beam of radiation, said prism inherently providing on said interface base a substantially complete reflection of said electromagnetic beam of radiation exciting said scintillating particulates attached to said immunological material, a measure of said excitement correlated to a measure of said attached immunological linking material.
Claim 2
Dependent Claim
123 words
A transparent optical container system for use in the non-destructive biological fluid assay of immunological attractive material by excitement of scintillating particulates and the measurement of said excitement, the improvement wherein: said transparent container having a side provided with a section forming a quadrilateral prism, said prism having an interface base provided with immunological linking material for attachment to said scintillating particulates, said prism inherently providing an angle of incidence greater than the critical angle for an electromagnetic beam of radiation, said prism inherently providing on said interface base a substantially complete reflection of said electromagnetic beam of radiation exciting said scintillating particulates attached to said immunological material, a measure of said excitement correlated to a measure of said attached immunological linking material.
Claim 3
Dependent Claim
11 words
The container of claim 1 wherein said immunological material is antigen.
Claim 4
Dependent Claim
11 words
The container of claim 1 wherein said immunological material is antibody.
Claim 5
Dependent Claim
20 words
The container of claim 1 wherein said interface base of said prism is the surface of a replaceable glass slide.
Claim 6
Dependent Claim
14 words
The container of claim 1 wherein said interface base is provided with a foil.
Claim 7
Dependent Claim
15 words
The container of claim 1 wherein said interface base is provided with an optical fiber.
Claim 8
Dependent Claim
14 words
The container of claim 1 wherein said interface base is surrounded by reflective surfaces.