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US4450236A
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Asporogenic mutant of Bacillus subtilis useful as a host in a host-vector system
Assignee: Unilever Bestfoods North America
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Filing: 1982-04-21
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Granted: 1984-05-22
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Expires: 2002-04-21
Abstract
Abstract A biologically pure strain of asporogenous B. subtilis DE101 (ATCC 39,095) is provided. This strain, which shows a low frequency of reversion to spore formers, is suitable for a host providing a high level of biological containment in a host-vector system for use in recombinant DNA methodology.
Inventors
- 👤 Donald H. Dean
- 👤 Daniel M. Ellis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 11:04
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/75; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
61 words
A biologically pure culture of asporogenous B. subtilis DE101 (ATCC 39,095) suitable for use as a host component in a host-vector system characterized in that it has a frequency of reversion to spore formers of less than about 10 -7 when grown under conditions of aeration and having the following genetic markers: thy A1, thy B1, pyr D1, and spo OAΔ677.
Claim 2
Dependent Claim
61 words
A biologically pure culture of asporogenous B. subtilis DE101 (ATCC 39,095) suitable for use as a host component in a host-vector system characterized in that it has a frequency of reversion to spore formers of less than about 10 -7 when grown under conditions of aeration and having the following genetic markers: thy A1, thy B1, pyr D1, and spo OAΔ677.
Claim 3
Dependent Claim
38 words
A biologically pure culture of asporogenous B. subtillis DE2 (ATCC 39,092) useful as an intermediate for preparation of asporogenous host components of host-vector systems characterized in that it contains the following genetic markers: pyr D1 and spo OAΔ677.
Claim 4
Dependent Claim
38 words
A biologically pure culture of asporogenous B. subtillis DE2 (ATCC 39,092) useful as an intermediate for preparation of asporogenous host components of host-vector systems characterized in that it contains the following genetic markers: pyr D1 and spo OAΔ677.
References Cited (Backward Citations)
US4237224A
Cited
US4302544A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-22 | 1987-11-21 | 1988-05-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-22 | 1991-11-21 | 1992-05-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-22 | 1995-11-21 | 1996-05-22 | $7700.00 | Window Closed / Lapsed |